Isolation of plasmids in Legionella pneumophila and Legionella-like organisms

Mikesell, Perry; Ezzell, John W.; Knudson, Gregory B.

doi:10.1128/iai.31.3.1270-1272.1981

Cited by 18 publications

(2 citation statements)

References 18 publications

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“…Several groups have observed plasmids in clinical and environmental Legionella pneumophila isolates (1,3,5,7,8). Plasmid analysis of L. pneumophila serogroup 1 (LP-1) isolates from diverse geographic loci has revealed 85-, 84-, 80-, 60-, and 43-megadalton (Mdal) plasmids (3,5,8). Studies conducted by one group (3) have correlated plasmid content with differential virulence and surface antigen composition, but overall, specific gene products or functions have yet to be assigned to any of these plasmids.…”

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confidence: 99%

Plasmid profiles of clinical and environmental isolates of Legionella pneumophila serogroup 1

Maher¹,

Plouffe²,

Para³

1983

J Clin Microbiol

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Clinical and environmental Legionella pneumophila serogroup 1 isolates from a single water source in Columbus, Ohio, exhibited five different plasmid profiles. The multiplicity of plasmid profiles observed within a single geographic area and a skewed distribution of isolates bearing these plasmid profiles within this area suggest that plasmid analyses will be useful in the study of the epidemiology of Legionnaires disease and the environmental distribution of legionellae.

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mentioning

confidence: 99%

Plasmid profiles of clinical and environmental isolates of Legionella pneumophila serogroup 1

Maher¹,

Plouffe²,

Para³

1983

J Clin Microbiol

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“…The majority (61%) of these environmental isolates contained a single 80-Mdal plasmid. The rapid, simple alkaline sodium dodecyl sulfate extraction procedure of Kado and Liu (7) allowed a more efficient recovery of plasmid DNA than did other extraction procedures previously used for the study of plasmids in Legionellaceae (1,8,10). Oddly, in none of the isolates obtained from 23 patients with Legionnaires disease could a plasmid be detected.…”

mentioning

confidence: 99%

Plasmid and surface antigen markers of endemic and epidemic Legionella pneumophila strains

Brown¹,

Vickers²,

Elder³

et al. 1982

J Clin Microbiol

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Environmental and clinical isolates of Legionella pneumophila obtained from the Pittsburgh Veterans Administration Medical Center were studied for the presence of plasmids and for unique surface antigens. The majority of environmental isolates contained a single 80-megadalton plasmid. After an epidemic of nosocomial Legionnaires disease subsided in the Spring of 1981, plasmid-bearing environmental isolates persisted in the environment. Whereas L. pneumophila could not be reisolated from most sites with plasmidless isolates. During this epidemic the attack rate was highest on wards with plasmidless isolates. All clinical isolates were plasmidless. Strains were serotyped by the indirect immunofluorescence method with serum from a single immunized rat which was used both without absorption and after absorption with various plasmid-bearing and plasmidless isolates. These studies suggested that a plasmid-associated surface antigen was present and that the most common plasmidless environmental serotype was similar to the epidemic clinical serotype.

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Legionella

Winn¹

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Le.gi.on. el' la . M.L. n. legio legion or army; M.L. dim. ending ‐ ella ; M.L. fem. n. Legionella small legion or army. Proteobacteria / Gammaproteobacteria / Legionellales / Legionellaceae / Legionella As the only genus in the family, the definition of Legionella is identical to that of Legionellaceae . Aerobic. l ‐cysteine‐HCl and iron salts are required for growth . Oxidase negative or weakly positive. Nitrates are not reduced. Urease negative. Gelatin is liquefied by most species. Chemoorganotrophic, using amino acids as carbon and energy sources. Carbohydrates are neither fermented nor oxidized (Tables BXII.γ.56 and BXII.γ.57). Branched chain fatty acids predominate in whole cell hydrolysate (Table BXII.γ.58). All species contain ubiquinones with 9–14 isoprene units in the side chains (Table BXII.γ.59). The mol % G + C of the DNA is : 38–52. Type species : Legionella pneumophila Brenner, Steigerwalt and McDade 1979, 658.

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Isolation of plasmids in Legionella pneumophila and Legionella-like organisms

Cited by 18 publications

References 18 publications

Plasmid profiles of clinical and environmental isolates of Legionella pneumophila serogroup 1

Plasmid profiles of clinical and environmental isolates of Legionella pneumophila serogroup 1

Plasmid and surface antigen markers of endemic and epidemic Legionella pneumophila strains

Legionella

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