Isolation of Procine Urinary Kallikrein

Tschesche, Harald; Mair, G.; Förg-Brey, B.; Fritz, Hans

doi:10.1007/978-1-4684-3267-1_16

Cited by 4 publications

(2 citation statements)

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“…8350; cristalline bovine trypsin, Novo Industri; guinea pig coagulation gland kallikrein and guinea pig submandibular kallikrein were gifts from Dr. C. Moriwaki, Tokyo and Dr. M. Lemon, Bristol. Pig urinary kallikrein and pig submandibular kallikrein preparations were isolated as described (4,5 …”

Section: Methodsmentioning

confidence: 99%

Development of a Radioimmunoassay for Pig Pancreatic Kallikrein

Fink¹,

Güttel²

1978

Clinical Chemistry and Laboratory Medicine

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Summary: A radioimmunoassay for the determination of pig pancreatic kallikrein was developed. The chloramine-T method was employed for the labelling of the antigen with 12S I. The assay allows the determination of kallikrein in concentrations as low as 0.4 /zg/1. Pig urinary and pig submandibular kallikreins are indistinguishable from pig pancreatic kallikrein by the assay. No cross reactivity was observed for bovine trypsin and chymotrypsin, porcine trypsin and kallikreins of guinea pig submandibular glands and guinea pig coagulation glands. Because of the high specificity of the assay, which is not attainable with conventional assays based on the enzymatic activity, the radioimmunoassay is highly suited for investigations into the physiological role and the pharmacological mechanism of action of pig glandular kallikreins. Entwicklung eines Radioimmunoassay s für Kallikrein aus SchweinepankreasZusammenfassung: Für die Bestimmung von Schweinepankreaskallikrein wurde ein Radioimmunoassay entwickelt. Die Markierung des Antigens mit 12S

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Section: Methodsmentioning

confidence: 99%

Development of a Radioimmunoassay for Pig Pancreatic Kallikrein

Fink¹,

Güttel²

1978

Clinical Chemistry and Laboratory Medicine

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“…Hog urinary kallikrein had been isolated by conventional methods following its esterolytic activity on the synthetic substrate BzArgOEt' 14 1 and an antibody could be raised in rabbits' 15 !. The physicochemical characterization, amino acid composition and enzyme kinetic data for BzArgOEt and other synthetic substrates as well as for enzyme inhibition by natural and synthetic inhibitors have been reported' 15 !.…”

Section: Verwendung Von Hydrophober Chromatographie Zur Reinigung Vonmentioning

confidence: 99%