Isolation of Pseudomonas oleovorans Carrying Multidrug Resistance Proteins MdtA and MdtB from Wastewater

Abstract

The pollution of industrial wastewater has become a global issue in terms of economic development and ecological protection. Pseudomonas oleovorans has been studied as a bacterium involved in the treatment of petroleum pollutants. Our study aimed to investigate the physicochemical properties and drug resistance of Pseudomonas oleovorans isolated from industrial wastewater with a high concentration of sulfate compounds. Firstly, Pseudomonas oleovorans was isolated and then identified using matrix-assisted flight mass spectrometry and 16S rDNA sequencing. Then, biochemical and antibiotic resistance analyses were performed on the Pseudomonas oleovorans, and a microbial high-throughput growth detector was used to assess the growth of the strain. Finally, PCR and proteomics analyses were conducted to determine drug-resistance-related genes/proteins. Based on the results of the spectrum diagram and sequencing, the isolated bacteria were identified as Pseudomonas oleovorans and were positive to reactions of ADH, MTE, CIT, MLT, ONPG, and ACE. Pseudomonas oleovorans was sensitive to most of the tested antibiotics, and its resistance to SXT and CHL and MIN and TIM was intermediate. The growth experiment showed that Pseudomonas oleovorans had a good growth rate in nutrient broth. Additionally, gyrB was the resistance gene, and mdtA2, mdtA3, mdtB2, mdaB, and emrK1 were the proteins that were closely associated with the drug resistance of Pseudomonas oleovorans. Our results show the biochemical properties of Pseudomonas oleovorans from industrial wastewater with a high concentration of sulfate compounds and provide a new perspective for Pseudomonas oleovorans to participate in biological removal of chemical pollutants in industrial wastewater.

The characteristics of the novel bacterial strain Pseudomonas mendocina isolated from freshwater aquaculture farm

“…Proteomics found that P. oleovorans has 1781 proteins and P. mendocina has 1745 proteins, but the two are different in mass spectrometry, 16S genes, and several biochemical drug resistance indicators. Considering the close genetic relationship between these two bacteria and the limited selection of genes, it is understandable that there are some similarities, but the two bacteria bring us a very good topic to further study the evolution and function of Pseudomonas oleovorans and Pseudomonas mendocina [37].…”

Genomic and Proteomic Analysis of Pseudomonas aeruginosa Isolated from Industrial Wastewater to Assess Its Resistance to Antibiotics

“…Increasing urbanization and industrialization have brought domestic sewage and industrial wastewater discharge into local water sources and groundwater. Water pollution not only aggravates the deterioration of the ecological environment but also has a certain impact on human health [1]. The increase in industrial wastewater containing organic pollutants further increases toxicity of ecosystems.…”