Isolation of Pure DNA for Metagenomic Study from Industrial Polluted Sites: A New Approach for Monitoring the Microbial Community and Pollutants

Chandra, Ram; Yadav, Sheelu

doi:10.1201/b19243-19

Cited by 6 publications

(3 citation statements)

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“…Thus, these proteins may inhibit fungal growth through metal ion sequestration. These proteins can also protect against oxidative damage and other abiotic stresses which result in generation of ROS (Tripathi et al, 2015 ). Other genes located on LG3 that exhibited significant up–regulation in MS after pathogen inoculation are MDP0000133552, coding for a late embryogenesis abundant (LEA) protein containing a harpin–inducible domain, and MDP0000324718, which codes for an ethylene–responsive transcription factor.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Response of Resistant (PI613981–Malus sieversii) and Susceptible (“Royal Gala”) Genotypes of Apple to Blue Mold (Penicillium expansum) Infection

et al. 2017

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Malus sieversii from Central Asia is a progenitor of the modern domesticated apple (Malus × domestica). Several accessions of M. sieversii are highly resistant to the postharvest pathogen Penicillium expansum. A previous study identified the qM–Pe3.1 QTL on LG3 for resistance to P. expansum in the mapping population GMAL4593, developed using the resistant accession, M. sieversii –PI613981, and the susceptible cultivar “Royal Gala” (RG) (M. domestica), as parents. The goal of the present study was to characterize the transcriptomic response of susceptible RG and resistant PI613981 apple fruit to wounding and inoculation with P. expansum using RNA–Seq. Transcriptomic analyses 0–48 h post inoculation suggest a higher basal level of resistance and a more rapid and intense defense response to wounding and wounding plus inoculation with P. expansum in M. sieversii –PI613981 than in RG. Functional analysis showed that ethylene–related genes and genes involved in “jasmonate” and “MYB–domain transcription factor family” were over–represented in the resistant genotype. It is suggested that the more rapid response in the resistant genotype (Malus sieversii–PI613981) plays a major role in the resistance response. At least twenty DEGs were mapped to the qM–Pe3.1 QTL (M × d v.1: 26,848,396–28,424,055) on LG3, and represent potential candidate genes responsible for the observed resistance QTL in M. sieversii–PI613981. RT–qPCR of several of these genes was used to validate the RNA–Seq data and to confirm their higher expression in MS0.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic Response of Resistant (PI613981–Malus sieversii) and Susceptible (“Royal Gala”) Genotypes of Apple to Blue Mold (Penicillium expansum) Infection

et al. 2017

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“…Plant metallothioneins are currently categorized into four types that slightly differ in a structure of cysteine-rich domains (Leszczyszyn et al 2013 ). The role of plant MTs is generally attributed to the homeostasis of essential metals (Se, Zn, Ni, and Cu), and detoxification of xenobiotic metals (Cd, Hg, and Ag) (Tripathi et al 2015 ). In recent years, great number of MT and MT-like gene sequences became available in databases and numerous studies reported on improved heavy metal tolerance in plants overexpressing MTs transgenes (Leszczyszyn et al 2013 ).…”

Section: Strategies For Improved Efficiency Of Phytoremediationmentioning

confidence: 99%

Recent strategies of increasing metal tolerance and phytoremediation potential using genetic transformation of plants

Koźmińska

Wiszniewska

Hanus-Fajerska

et al. 2018

Plant Biotechnol Rep

150

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Avoidance and reduction of soil contamination with heavy metals is one of the most serious global challenges. Nowadays, science offers us new opportunities of utilizing plants to extract toxic elements from the soil by means of phytoremediation. Plant abilities to uptake, translocate, and transform heavy metals, as well as to limit their toxicity, may be significantly enhanced via genetic engineering. This paper provides a comprehensive review of recent strategies aimed at the improvement of plant phytoremediation potential using plant transformation and employing current achievements in nuclear and cytoplasmic genome transformation. Strategies for obtaining plants suitable for effective soil clean-up and tolerant to excessive concentrations of heavy metals are critically assessed. Promising directions in genetic manipulations, such as gene silencing and cis- and intragenesis, are also discussed. Moreover, the ways of overcoming disadvantages of phytoremediation using genetic transformation approachare proposed. The knowledge gathered here could be useful for designing new research aimed at biotechnological improvement of phytoremediation efficiency.

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“…PCs merupakan peptida yang disintesis secara enzimatik dari glutathione, sedangkan metallothionein merupakan produk yang dihasilkan langsung dari ekspresi gen (Koźmińska et al, 2018). Metallothionein pada tumbuhan, secara umum, berperan dalam homeostasis logam-logam esensial (Se, Zn, Ni, dan Cu) dan dalam detoksifikasi logam xenobiotic, seperti Cd, Hg, dan Ag (Tripathi et al, 2015 Hasil analisis profil genetik menggunakan primer spesifik untuk gen metallothionein (mt-gene) menunjukkan bahwa tidak adanya pita DNA spesifik pada semua sampel baik dari lokasi bekas tambang emas maupun lokasi non-tambang. Hal ini diduga karena tidak adanya sekuens gen yang homolog pada sampel uji dengan sekuens gen target yang digunakan.…”

Section: Pendahuluanunclassified

Analysis of Genetic Profiles of Heavy Metal Phytememediator Plants From Gold Mining Areas

Winarti

Neneng

Gunawan

et al. 2020

BSc

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The resistance and survival ability of phytoremediator plants in a polluted environment is thought to be a form of genetic adaptation. This research aimed: (1) to identify the dominant plant species in ex-gold mining area; (2) to analyze the genetic profile of phytoremediator plants from ex-gold mining area; and (3) to compare the genetic profile of heavy metal phytoremediator plants from ex-gold mining area with the same plant species from the non-mining area. The samples of this study were Cyperus sp., Lycopodium sp., and Melastoma sp. The research procedures carried out include sample collection, DNA isolation, DNA amplification with PCR, and DNA visualization with electrophoresis. The results show that the dominant plant species of ex-gold mining area are Cyperus sp., Lycopodium sp., and Melastoma sp. The genetic profile analysis of dominant plant species of ex-gold mining area shows that no DNA bands appeared from the target gene as the result of amplification using specific primers of Metallothionein gene. The result of RAPD analysis using OPA-04 universal primers show that at 500-750 bp there are differences in DNA bands that appeared between the samples. DNA bands that appeared in the genetic profile of phytoremediator plants are thought to be the representation of the gene that responsible for heavy metals tolerance.

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Isolation of Pure DNA for Metagenomic Study from Industrial Polluted Sites: A New Approach for Monitoring the Microbial Community and Pollutants

Cited by 6 publications

References 1 publication

Transcriptomic Response of Resistant (PI613981–Malus sieversii) and Susceptible (“Royal Gala”) Genotypes of Apple to Blue Mold (Penicillium expansum) Infection

Transcriptomic Response of Resistant (PI613981–Malus sieversii) and Susceptible (“Royal Gala”) Genotypes of Apple to Blue Mold (Penicillium expansum) Infection

Recent strategies of increasing metal tolerance and phytoremediation potential using genetic transformation of plants

Analysis of Genetic Profiles of Heavy Metal Phytememediator Plants From Gold Mining Areas

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