Isolation of putative defense-related genes from Arabidopsis thaliana and expression in fungal elicitor-treated cells

Abstract

Numerous Arabidopsis genes have been cloned that correspond to putative pathogen defense-related genes identified in parsley (Petroselinum crispum). Treatment of Arabidopsis cells with fungal elicitor leads to rapid accumulation of the respective mRNAs with time courses comparable to those observed for their counterparts in parsley. Evolutionary sequence conservation of many of these genes in several plant species suggests they code for important plant functions.

The Arabidopsis Transcription Factor MYB12 Is a Flavonol-Specific Regulator of Phenylpropanoid Biosynthesis

“…The Arabidopsis (Arabidopsis thaliana) cell line At7 was described previously (Trezzini et al, 1993;Hartmann et al, 1998). Seedlings for quantitative real time RT-PCR, HPLC analysis, and photometric anthocyanin analysis were grown in petri dishes on Whatman-paper soaked with Murashige and Skoog medium lacking Suc under sterile conditions.…”

Differential combinatorial interactions of cis-acting elements recognized by R2R3-MYB, BZIP, and BHLH factors control light-responsive and tissue-specific activation of phenylpropanoid biosynthesis genes

“…Plant material was harvested, frozen in liquid nitrogen and stored at )80°C until use. The A. thaliana cell line At7 which grows in the dark has been described previously (Trezzini et al, 1993;Hartmann et al, 1998). To block de novo protein synthesis, protoplasts derived from At7 cells were treated with 10 lM cycloheximide; the drug was added to the culture 30 min before the onset of light irradiation.…”

Arabidopsis thaliana defense-related protein ELI3 is an aromatic alcohol:NADP + oxidoreductase

“…The presence of this gene in other plant species, such as potato (Solanum tuberosum), alfalfa (Medicago sativa), and Arabidopsis thaliana (At) was also demonstrated (6). Subsequently, two sequence-related counterparts of eli3 in parsley were isolated from A. thaliana (Ateli3-1 and Ateli3-2), and their expression was shown to be induced by fungal elicitor in cultured A. thaliana cells (6). Additional evidence for an important role of the eli3 gene product in plant disease resistance came from genetic studies demonstrating that eli3 expression was dependent on the presence of the RPMI resistance gene in A. thaliana (7).…”

“…Expression of this gene was shown to be rapidly and transiently stimulated in cultured parsley cells upon treatment with a cell wall preparation (elicitor) from the phytopathogenic fungus Phytophthora sojae, and histochemical studies revealed local and rapid accumulation of ELI3 mRNA around fungal infection sites in parsley leaves (5). The presence of this gene in other plant species, such as potato (Solanum tuberosum), alfalfa (Medicago sativa), and Arabidopsis thaliana (At) was also demonstrated (6). Subsequently, two sequence-related counterparts of eli3 in parsley were isolated from A. thaliana (Ateli3-1 and Ateli3-2), and their expression was shown to be induced by fungal elicitor in cultured A. thaliana cells (6).…”