Isolation of pyrene degrading Achromobacter xylooxidans and characterization of metabolic product

Tiwari, Jitendra Nath; Reddy, Mudiam Mohana Krishna; Patel, Devendra Kumar; Jain, Shubha; Murthy, Ramesh; Manickam, Natesan

doi:10.1007/s11274-010-0350-6

Cited by 28 publications

(6 citation statements)

References 29 publications

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“…Burkholderiales’ ability to degrade aromatic compounds has been previously studied [ 4 , 41 – 44 ]. Its nutritional versatility and ability to use a wide range of organic compounds as carbon sources has led to the use of Burkholderia strains for biodegradation of environmental pollutants [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Assigning ecological roles to the populations belonging to a phenanthrene-degrading bacterial consortium using omic approaches

et al. 2017

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The present study describes the behavior of a natural phenanthrene-degrading consortium (CON), a synthetic consortium (constructed with isolated strains from CON) and an isolated strain form CON (Sphingobium sp. AM) in phenanthrene cultures to understand the interactions among the microorganisms present in the natural consortium during phenanthrene degradation as a sole carbon and energy source in liquid cultures. In the contaminant degradation assay, the defined consortium not only achieved a major phenanthrene degradation percentage (> 95%) but also showed a more efficient elimination of the intermediate metabolite. The opposite behavior occurred in the CON culture where the lowest phenanthrene degradation and the highest HNA accumulation were observed, which suggests the presence of positive and also negative interaction in CON. To consider the uncultured bacteria present in CON, a metagenomic library was constructed with total CON DNA. One of the resulting scaffolds (S1P3) was affiliated with the Betaproteobacteria class and resulted in a significant similarity with a genome fragment from Burkholderia sp. HB1 chromosome 1. A complete gene cluster, which is related to one of the lower pathways (meta-cleavage of catechol) involved in PAH degradation (ORF 31–43), mobile genetic elements and associated proteins, was found. These results suggest the presence of at least one other microorganism in CON besides Sphingobium sp. AM, which is capable of degrading PAH through the meta-cleavage pathway. Burkholderiales order was further found, along with Sphingomonadales order, by a metaproteomic approach, which indicated that both orders were metabolically active in CON. Our results show the presence of negative interactions between bacterial populations found in a natural consortium selected by enrichment techniques; moreover, the synthetic syntrophic processing chain with only one microorganism with the capability of degrading phenanthrene was more efficient in contaminant and intermediate metabolite degradation than a generalist strain (Sphingobium sp. AM).

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Section: Discussionmentioning

confidence: 99%

Assigning ecological roles to the populations belonging to a phenanthrene-degrading bacterial consortium using omic approaches

et al. 2017

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“…Most of the isolates belonged to the genus Pseudomonas , although Comamonas , Sphingomonas , Stenotrophomonas and Delftia were also found. Tiwari et al (2010) studied a bacterium characterized as A. xylosoxidans , isolated from an oil refinery effluent sludge and capable of aerobic degradation of PYR. Ghevariya et al (2011) isolated a multiple PAH degrading halotolerant A. xylosoxidans , which was isolated from crude oil polluted saline site and exhibited 86% chrysene degradation.…”

Section: Resultsmentioning

confidence: 99%

Bioaugmentation of PAH-Contaminated Soils With Novel Specific Degrader Strains Isolated From a Contaminated Industrial Site. Effect of Hydroxypropyl-β-Cyclodextrin as PAH Bioavailability Enhancer

et al. 2019

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A PAHs-contaminated industrial soil was analyzed using PCR amplification of the gene 16S ribosomal RNA for the detection and identification of different isolated bacterial strains potentially capable of degrading PAHs. Novel degrader strains were isolated and identified as Achromobacter xylosoxidans 2BC8 and Stenotrophomonas maltophilia JR62, which were able to degrade PYR in solution, achieving a mineralization rate of about 1% day–1. A. xylosoxidans was also able to mineralize PYR in slurry systems using three selected soils, and the total extent of mineralization (once a plateau was reached) increased 4.5, 21, and 57.5% for soils LT, TM and CR, respectively, regarding the mineralization observed in the absence of the bacterial degrader. Soil TM contaminated with PYR was aged for 80 days and total extent of mineralization was reduced (from 46 to 35% after 180 days), and the acclimation period increased (from 49 to 79 days). Hydroxypropyl-ß-cyclodextrin (HPBCD) was used as a bioavailability enhancer of PYR in this aged soil, provoking a significant decrease in the acclimation period (from 79 to 54 days) due to an increase in PYR bioavailable fraction just from the beginning of the assay. However, a similar global extension of mineralization was obtained. A. xylosoxidans was then added together with HPBCD to this aged TM soil contaminated with PYR, and the total extent of mineralization decreased to 25% after 180 days, possibly due to the competitive effect of endogenous microbiota and the higher concentration of PYR in the soil solution provoked by the addition of HPBCD, which could have a toxic effect on the A. xylosoxidans strain.

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“…, and Achromobacter sp. . Few species of Bacillus have also been isolated from PAHs contaminated soil samples .…”

Section: Discussionmentioning

confidence: 99%

Isolation and identification of Bacillus megaterium YB3 from an effluent contaminated site efficiently degrades pyrene

Meena

Sharma

Gupta³

et al. 2016

J. Basic Microbiol.

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Industrial effluents contaminated sites may serve as repositories of ecologically adapted efficient pyrene degrading bacteria. In the present study, six bacterial isolates from industrial effluents were purified using serial enrichment technique and their pyrene degrading potential on pyrene supplemented mineral salt medium was assessed. 16S rRNA sequence analysis showed that they belong to four bacterial genera, namely Acinetobacter, Bacillus, Microbacterium, and Ochrobactrum. Among these isolates, Bacillus megaterium YB3 showed considerably good growth and was further evaluated for its pyrene-degrading efficiency. B. megaterium YB3 could degrade 72.44% of 500 mg L(-1) pyrene within 7 days. GC-MS analysis of ethyl acetate extracted fractions detected two relatively less toxic metabolic intermediates of the pyrene degradation pathway. B. megaterium YB3 also tested positive for catechol 1, 2-dioxygenase and aromatic-ring-hydroxylating dioxygenase indole-indigo conversion assays. Considering the ability and efficiency of B. megaterium YB3 to degrade high pyrene content, the strain can be used as a tool to develop bioremediation technologies for the effective biodegradation of pyrene and possibly other PAHs in the environment.

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Isolation of pyrene degrading Achromobacter xylooxidans and characterization of metabolic product

Cited by 28 publications

References 29 publications

Assigning ecological roles to the populations belonging to a phenanthrene-degrading bacterial consortium using omic approaches

Assigning ecological roles to the populations belonging to a phenanthrene-degrading bacterial consortium using omic approaches

Bioaugmentation of PAH-Contaminated Soils With Novel Specific Degrader Strains Isolated From a Contaminated Industrial Site. Effect of Hydroxypropyl-β-Cyclodextrin as PAH Bioavailability Enhancer

Isolation and identification of Bacillus megaterium YB3 from an effluent contaminated site efficiently degrades pyrene

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