Isolation of rare cells from cell mixtures by dielectrophoresis

Gascoyne, Peter R. C.; Noshari, Jamileh; Anderson, Thomas; Becker, Frederick F.

doi:10.1002/elps.200800373

Cited by 410 publications

(372 citation statements)

References 56 publications

(73 reference statements)

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“…[2][3][4] For most biological cells consisting of cytoplasm and plasma membrane, spherical single-shell model has been widely used by researchers to represent blood cells 5,6 and cancer cells. [7][8][9][10][11] In some cases, such as plants and most micro-organisms where the cell is surrounded by a cell wall, two-shell dielectric models were used. 3 Some bacteria, like Escherichia coli, are rod-shape; hence, prolate spheroidal core-shell models 12,13 were developed and applied.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic capture voltage spectrum for measurement of dielectric properties and separation of cancer cells

Wu¹,

Yung²,

Lim³

2012

Biomicrofluidics

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In this paper, a new dielectrophoresis (DEP) method based on capture voltage spectrum is proposed for measuring dielectric properties of biological cells. The capture voltage spectrum can be obtained from the balance of dielectrophoretic force and Stokes drag force acting on the cell in a microfluidic device with fluid flow and strip electrodes. The method was demonstrated with the measurement of dielectric properties of human colon cancer cells . From the capture voltage spectrum, the real part of Clausius-Mossotti factor of HT-29 cells for different frequencies of applied electric field was obtained. The dielectric properties of cell interior and plasma membrane were then estimated by using single-shell dielectric model. The cell interior permittivity and conductivity were found to be insensitive to changes in the conductivity of the medium in which the cells are suspended, but the measured permittivity and conductivity of cell membrane were found to increase with the increase of medium conductivity. In addition, the measurement of capture voltage spectrum was found to be useful in providing the optimum operating conditions for separating HT-29 cells from other cells (such as red blood cells) using dielectrophoresis. V C 2012 American Institute of Physics. [http://dx

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Section: Introductionmentioning

confidence: 99%

Dielectrophoretic capture voltage spectrum for measurement of dielectric properties and separation of cancer cells

Wu¹,

Yung²,

Lim³

2012

Biomicrofluidics

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“…We used MDA-MB-231 cell parameters as estimated in literature 21,34,35 and a medium conductivity of 10 mS/m corresponding to the DEP Buffer generally used in literature. 5,7,[24][25][26] Indeed, it is well known that low conductivity medium increases the efficiency of electroporation 36 and DEP reduces electrothermal effects 37,38 and water electrolysis. The magnitude of the dielectrophoretic force depends on the value of the real part of the CM factor (Eq.…”

Section: A Cell Motion Modelmentioning

confidence: 99%

Dielectrophoretic capture of low abundance cell population using thick electrodes

et al. 2015

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Enrichment of rare cell populations such as Circulating Tumor Cells (CTCs) is a critical step before performing analysis. This paper presents a polymeric microfluidic device with integrated thick Carbon-PolyDimethylSiloxane composite (C-PDMS) electrodes designed to carry out dielectrophoretic (DEP) trapping of low abundance biological cells. Such conductive composite material presents advantages over metallic structures. Indeed, as it combines properties of both the matrix and doping particles, C-PDMS allows the easy and fast integration of conductive microstructures using a soft-lithography approach while preserving O 2 plasma bonding properties of PDMS substrate and avoiding a cumbersome alignment procedure. Here, we first performed numerical simulations to demonstrate the advantage of such thick C-PDMS electrodes over a coplanar electrode configuration. It is well established that dielectrophoretic force (F DEP ) decreases quickly as the distance from the electrode surface increases resulting in coplanar configuration to a low trapping efficiency at high flow rate. Here, we showed quantitatively that by using electrodes as thick as a microchannel height, it is possible to extend the DEP force influence in the whole volume of the channel compared to coplanar electrode configuration and maintaining high trapping efficiency while increasing the throughput. This model was then used to numerically optimize a thick C-PDMS electrode configuration in terms of trapping efficiency. Then, optimized microfluidic configurations were fabricated and tested at various flow rates for the trapping of MDA-MB-231 breast cancer cell line. We reached trapping efficiencies of 97% at 20 ll/h and 78.7% at 80 ll/h, for 100 lm thick electrodes. Finally, we applied our device to the separation and localized trapping of CTCs (MDA-MB-231) from a red blood cells sample (concentration ratio of 1:10). V C 2015 AIP Publishing LLC.

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“…21 Dielectrophoresis (DEP) is a powerful technique that has been used for the separation and concentration of different bioparticles including bacteria, DNA, and infected cells from blood. 9,[22][23][24][25][26][27][28] A significant challenge when using DEP for particle trapping is the need for low electrically conductive media, since most, if not all, of the relevant biological samples such as blood and urine feature high electrical conductivity, re-suspension of the sample in optimized DEP buffers is necessary. Another challenge in DEP has been improving throughput.…”

Section: Introductionmentioning

confidence: 99%

Enrichment of diluted cell populations from large sample volumes using 3D carbon-electrode dielectrophoresis

et al. 2016

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Here, we report on an enrichment protocol using carbon electrode dielectrophoresis to isolate and purify a targeted cell population from sample volumes up to 4 ml. We aim at trapping, washing, and recovering an enriched cell fraction that will facilitate downstream analysis. We used an increasingly diluted sample of yeast, 10 6 -10 2 cells/ml, to demonstrate the isolation and enrichment of few cells at increasing flow rates. A maximum average enrichment of 154.2 6 23.7 times was achieved when the sample flow rate was 10 ll/min and yeast cells were suspended in low electrically conductive media that maximizes dielectrophoresis trapping. A COMSOL Multiphysics model allowed for the comparison between experimental and simulation results. Discussion is conducted on the discrepancies between such results and how the model can be further improved. Published by AIP Publishing.

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Isolation of rare cells from cell mixtures by dielectrophoresis

Cited by 410 publications

References 56 publications

Dielectrophoretic capture voltage spectrum for measurement of dielectric properties and separation of cancer cells

Dielectrophoretic capture voltage spectrum for measurement of dielectric properties and separation of cancer cells

Dielectrophoretic capture of low abundance cell population using thick electrodes

Enrichment of diluted cell populations from large sample volumes using 3D carbon-electrode dielectrophoresis

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