Isolation of Ready‐to‐Use Adipose‐Derived Stem Cell (ASC) Pellet for Clinical Applications and a Comparative Overview of Alternate Methods for ASC Isolation

Raposio, Edoardo; Bertozzi, Nicolò

doi:10.1002/cpsc.29

Cited by 44 publications

(31 citation statements)

References 19 publications

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“…The regenerative characteristics of adipose stem cells, the ease and the high availability of adipose tissue and the great diffusion of fat graft in the various medical disciplines have, in recent decades, been a major interest in new and more secure isolation techniques of stem cells [136] , [137] , [138] , [139] . The extraction and implantation of stem cells has reversed, and is revealing, very high regenerative capacity for a large number of different organs and tissues, opening up new perspectives for regenerative medicine.…”

Section: Discussionmentioning

confidence: 99%

The science behind autologous fat grafting

Bellini

Grieco

Raposio

2017

Annals of Medicine &Amp; Surgery

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171

163

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IntroductionAdipose grafting has undergone significant changes over time. Many different techniques have been followed by trying to improve the quality of the lipoaspirate and the survival of the fat graft after implantation.Material and methodsThe purpose of this review is to analyse the historical evolution of the surgical harvesting and implant technique, describing the changes that have brought significant improvements, revolutionizing the aesthetic and functional results obtainable.ResultsA standard fat grafting technique is commonly performed in three stages: harvesting of adipose tissue from a suitable donor site; processing of the lipoaspirate to eliminate cellular debris, acellular oil and excess of infiltrated solution, reinjection of the purified adipose tissue. The most widely used surgical technique was described by Coleman. He modified and corrected the methods and results of his predecessors and proposed an atraumatic protocol for the treatment of adipose tissue.He reported that the key to successful fat grafting lies in the technique. In addition, he noticed that adipose tissue was not only a good filler, but improved the quality of the skin. In fact, fat grafts demonstrated to have not only dermal filler properties but also regenerative potential owing to the presence of stem cells in fat tissue.ConclusionAdipose tissue, actually, is the closest to the ideal filler because it is readily available; easily obtainable, with low donor-site morbidity; repeatable; inexpensive; versatile; and biocompatible. There is an abundance of literature supporting the efficacy of fat grafting in both aesthetic and reconstructive cases. Recent studies have shown the utility of adipose-derived stem cells in the improvement of wound healing, describing their ability to regenerate soft tissues and their remodelling capacity provided by their unique cytokine and growth factor profiles.Despite ongoing concerns about survival and longevity of fat grafts after implantation and unpredictability of long-term outcome, fat has been successfully used as a filler in many differ clinic situation.

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Section: Discussionmentioning

confidence: 99%

The science behind autologous fat grafting

Bellini

Grieco

Raposio

2017

Annals of Medicine &Amp; Surgery

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171

163

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“…Nucleated cells in fat graft samples were isolated as previously described, 19 – 21 and resulting stromal vascular fraction cells were enumerated and used for the following assays.…”

Section: Methodsmentioning

confidence: 99%

Physical, Biochemical, and Biologic Properties of Fat Graft Processed via Different Methods

Fang

Patel

et al. 2020

Plastic and Reconstructive Surgery - Global Open

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Background: Clinical use of autologous fat for correction of soft-tissue defects in cosmetic and reconstructive procedures has grown in popularity. Graft processing is implicated as one of the variable factors affecting quality, viability, and subsequent graft survival. This study analyzed the in vitro physical and biologic characteristics of lipoaspirate processed using different techniques. Methods: Fresh lipoaspirates from patients with informed consent were processed by 4 methods: decantation, centrifugation, the REVOLVE System, and PureGraft. Processed fat grafts were analyzed for yield, composition, tissue particle size and morphology, and viability and function of adipocytes and stem cells. Fat tissue harvested from waste containers of REVOLVE and PureGraft and trapped on REVOLVE paddles was also evaluated. Results: Grafts produced by the filtration systems contained the highest percentage of fat tissue, whereas those from decantation contained the lowest percentage, although they have the highest volume yield. In addition, grafts from REVOLVE and PureGraft showed more large-sized particles (>1000 μm) than those from decantation or centrifugation. REVOLVE also preserved significantly higher populations of viable and functional adipocytes and stromal vascular fraction cells when compared with other processing methods. Tissue particles in waste containers of REVOLVE and PureGraft were mostly (>85%) <300 μm and demonstrated a minimal number of viable adipocytes and stem cells. Fat tissues trapped on REVOLVE paddles contained a higher percentage of noninjectable and fibrous collagen bundles. Conclusion: Different processing methods result in fat grafts with varying physical and biologic properties, which may contribute to fat graft viability and retention in vivo.

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“…The harvesting technique is currently the traditional liposuction, carried out in small quantities and with the syringes method to limit the traumas on adipocytes. Adipose-derived stem cells have potentially very high clinical applications in various medical and surgical specialties, justifying the present and future significant efforts on new techniques for isolating, collecting and maximizing these stem cells [119] , [120] , [121] , [122] .\\\\…”

Section: Discussionmentioning

confidence: 99%

A journey through liposuction and liposculture: Review

Bellini

Grieco

Raposio

2017

Annals of Medicine &Amp; Surgery

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105

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IntroductionNowadays, liposuction is the most frequently performed aesthetic surgery procedure in Western Countries. This technique has had rapid development since the 1970s, when it was experimented for the first time by A. and G. Fischer. It is currently widely used in clinical practice for many different situations in aesthetic, reconstructive and functional fields.Materials and methodsThis review aims to describe the historical evolution of liposuction by analyzing the transformation of the method in function of the introduction of innovative ideas or instruments. We have also focused on reporting the major clinical applications of this surgical technique, applicable to almost the entire body surface. We finally analyzed the complications, both major and minor, associated with this surgical technique.ResultsLiposuction is mainly used to correct deep and superficial fat accumulations and remodel the body contour. It has become an essential complementary technique to enhance the aesthetic result of many other aesthetic procedures such as reduction mammoplasty, abdominoplasty, brachioplasty, thigh lift and post bariatric body contouring. However, it can be largely used for the treatment of innumerable pathologies in reconstructive surgery such as lipomas, lipedema, lipodystrophies, pneudogynecomastia and gynecomastia, macromastia e gigantomastia, lymphedema and many others. The complication rate is very low, especially when compared with conventional excisional surgery and the major, complications are generally associated with improper performance of the technique and poor patient management before and after surgery.ConclusionLiposuction is a safe, simple and effective method of body contouring. It has enormous potential for its application in ablative and reconstructive surgery, far from the most common aesthetic processes with a very low complication rate.

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Isolation of Ready‐to‐Use Adipose‐Derived Stem Cell (ASC) Pellet for Clinical Applications and a Comparative Overview of Alternate Methods for ASC Isolation

Cited by 44 publications

References 19 publications

The science behind autologous fat grafting

The science behind autologous fat grafting

Physical, Biochemical, and Biologic Properties of Fat Graft Processed via Different Methods

A journey through liposuction and liposculture: Review

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