Isolation of retrovirus from patients with multiple sclerosis

Perron, Hervé; Lalande, B.; Gratacap, B.; Laurent, Angela M.; Genoulaz, O.; Gény, Christian; Mallaret, Martial; Schuller, E; Stoebner, P; Seigneurin, J. M.

doi:10.1016/0140-6736(91)92579-q

Cited by 128 publications

(89 citation statements)

References 4 publications

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“…This was followed by similar findings in monocyte cultures from a series of MS patients (10). Neither viral particles nor viral RT activity was found in control individuals.…”

supporting

confidence: 64%

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Molecular identification of a novel retrovirus repeatedly isolated from patients with multiple sclerosis

Perron

Garson²,

Bedin³

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

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The partial molecular characterization of multiple sclerosis (MS)-associated retrovirus (MSRV), a novel retrovirus previously called LM7, is reported. MSRV has been isolated repeatedly from leptomeningeal, choroid plexus and from Epstein-Barr virus-immortalized B cells of MS patients. A strategy based on reverse transcriptase PCR with RNA-purified extracellular virions yielded an initial pol fragment from which other regions of the retroviral genome were subsequently obtained by sequence extension. MSRVspecific PCR primers amplified a pol region from RNA present at the peak of reverse transcriptase activity, coinciding with extracellular viral particles in sucrose density gradients. The same sequence was detected in noncellular RNA from MS patient plasma and in cerebrospinal f luid from untreated MS patients. MSRV is related to, but distinct from, the endogenous retroviral sequence ERV9. Whether MSRV represents an exogenous retrovirus with closely related endogenous elements or a replication-competent, virion-producing, endogenous provirus is as yet unknown. Further molecular epidemiological studies are required to determine precisely the apparent association of virions containing MSRV RNA with MS.

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“…This was followed by similar findings in monocyte cultures from a series of MS patients (10). Neither viral particles nor viral RT activity was found in control individuals.…”

supporting

confidence: 64%

“…RT activity was tested as described (9)(10)(11)(12). cDNA Synthesis and ''Pan-Retro'' RT-PCR with Degenerate Primers.…”

mentioning

confidence: 99%

Molecular identification of a novel retrovirus repeatedly isolated from patients with multiple sclerosis

Perron

Garson²,

Bedin³

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

430

311

View full text Add to dashboard Cite

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“…The envelope of an HERV may either protect the host from exogenous retroviral infection in a receptor interference fashion [163] or dysregulate the local cellular immunity through a superantigen-encoded region, as proposed for type I diabetes [164]. A study has observed that the multiple sclerosis-associated retrovirus detected in the plasma of patients with multiple sclerosis [165,166] has high homology to an HERV [167], which was named HERV-W. Xenotransplantation techniques and gene therapy approaches based on retroviridae vectors may eventually tamper with the biology of HERVs [161]. Retroviral vectors may recombine with HERVs in patients, and generate a variety of possible adverse effects.…”

Section: Vector Systems Based On Retroviruses Lentiviruses and Foamimentioning

confidence: 99%

Latest Developments in Gene Transfer Technology: Achievements, Perspectives, and Controversies over Therapeutic Applications

et al. 2000

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“…activity Since a significant gliotoxicity associated with cultures from MS patients was observed, it was decided to analyze for any correlation between this cytotoxicity and the RT activity previously reported in MMCS from 'active' MS patients [13,17,30]. The results are presented for both the gliotoxic activity measured by the MTT assay on CLTT 1-1 cells (Fig.…”

Section: Correlation Of Cytotoxic Activity and Reverse Transcriptasementioning

confidence: 99%

“…A working hypothesis is that the cascade of pathological events as well as autoimmunity may be triggered by a viral agent. We have recently described retrovirus-like extracellular particles associated with reverse transcriptase (RT) activity in culture supernatants from patients with MS [12,13]. The first molecular data on this retrovirus (now designated MSRV, MS associated RetroVirus) have revealed phylogenic proximity with a human endogenous retroviral family represented by ERV-9 [14].…”

mentioning

confidence: 99%

Gliotoxicity, reverse transcriptase activity and retroviral RNA in monocyte/macrophage culture supernatants from patients with multiple sclerosis

et al. 1997

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In investigating a possible link between a novel retroviral agent (provisionally called MSRV), recently characterised in multiple sclerosis (MS), and the neuropathology of MS, it was found that there was a significant correlation between gliotoxicity and reverse transcriptase activity in monocyte/ macrophage culture supernatants (MMCS) unique to MS patients. MMCS from healthy controls and patients with other neurological diseases did not display either gliotoxicity or reverse transcriptase activity. The observed gliotoxic effect was an initial, intermediate filament network disorganization and subsequent cell death which was specific to astrocytes and oligodendrocytes. The reverse transcriptase activity and MSRVspecific RNA were observed during the first 2 weeks of culture in MMCS from patients with active MS. The further elucidation of the molecular form(s) of this gliotoxic factor and its original source may be crucial in elucidating important etiopathogenic mechanisms in MS.

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Isolation of retrovirus from patients with multiple sclerosis

Cited by 128 publications

References 4 publications

Molecular identification of a novel retrovirus repeatedly isolated from patients with multiple sclerosis

Molecular identification of a novel retrovirus repeatedly isolated from patients with multiple sclerosis

Latest Developments in Gene Transfer Technology: Achievements, Perspectives, and Controversies over Therapeutic Applications

Gliotoxicity, reverse transcriptase activity and retroviral RNA in monocyte/macrophage culture supernatants from patients with multiple sclerosis

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