2006
DOI: 10.4269/ajtmh.2006.75.732
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Isolation of Rickettsia Akari From Eschars of Patients With Rickettsialpox

Abstract: Rickettsialpox is a cosmopolitan, mite-borne, spotted fever rickettsiosis caused by Rickettsia akari. The disease is characterized by a primary eschar, fever, and a papulovesicular rash. Rickettsialpox was first identified in New York City in 1946 and the preponderance of recognized cases in the United States continues to originate from this large metropolitan center. The most recently isolated U.S. strain of R. akari was obtained more than a half century ago. We describe the culture and initial characterizati… Show more

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Cited by 51 publications
(44 citation statements)
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“…3,19 Most IHC tests use polyclonal anti-R rickettsii antibodies that will react with many different species of SFGR, including R rickettsii, R akari, R africae, R conorii, Rickettsia species 364D, and R parkeri, making the results of IHC studies insufficient for identifying the specific infecting agent. 3,5,6,18 Although IHC staining of the biopsy specimens from each of our patients showed evidence of infection with SFGR, specific identification of the infecting pathogen was not possible in 2 of the 3 cases because IHC stains are only SFGR specific ( Table 2). The acute and convalescent serologic tests are also only SFGR specific and have the same limitations as IHC staining.…”
Section: Casementioning
confidence: 99%
“…3,19 Most IHC tests use polyclonal anti-R rickettsii antibodies that will react with many different species of SFGR, including R rickettsii, R akari, R africae, R conorii, Rickettsia species 364D, and R parkeri, making the results of IHC studies insufficient for identifying the specific infecting agent. 3,5,6,18 Although IHC staining of the biopsy specimens from each of our patients showed evidence of infection with SFGR, specific identification of the infecting pathogen was not possible in 2 of the 3 cases because IHC stains are only SFGR specific ( Table 2). The acute and convalescent serologic tests are also only SFGR specific and have the same limitations as IHC staining.…”
Section: Casementioning
confidence: 99%
“…Rickettsia rickettsii strain Bitterroot, R. massiliae strain AZT80, R. rhipicephali strains 3-7-♀-6 and CA871, and 364D Rickettsia were cultivated in African green monkey kidney cells (E6-Vero), harvested, and purified from the host cells using centrifugation through 25-45% (w/v) Renografin (Nycomed, Inc., Princeton, NJ) gradients as described. 28 Purified rickettsiae were kept frozen in aliquots (10 mg of protein/mL) in sterile distilled water at -80°C until used for electrophoresis and Western blotting.…”
Section: Study Description In August 2007 and March 2008 The Los Anmentioning
confidence: 99%
“…R. akari, a well characterized member of the spotted fever group Rickettsia is the ethiologic agent of rickettsialpox, a benign zoonotic illness which has similar clinical manifestation like that of murine typhus. R. akari has been reported to be very common in metropolitan cities of eastern United States, Argentina, Eastern European countries like Turkey, Croatia, Ukraine, Russia, Mexico, and Asia where they have been severally reported [16][17][18][19][20][21][22][23][24][25][26][27]. To the best of our knowledge, the presence of R. akari and its closest relatives R. felis and R. australis has not been reported anywhere in the African continent.…”
Section: Discussionmentioning
confidence: 99%