Isolation of root-associated <i>Pseudomonas</i> and <i>Burkholderia</i> spp. with biocontrol and plant growth-promoting traits

Wakelin, Steven A.; Young, Sandra; Gerard, Emily; Mander, Carolyn; O’Callaghan, Maureen

doi:10.1080/09583157.2016.1248899

Cited by 9 publications

(7 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, PGPB can serve as a phytostimulator but potential traits of the PGPB can be altered genetically by different environmental stresses [5]. Consortium of newly isolated and already characterized PGPR will ensure their sustainable use in crop production [6].…”

Section: Introductionmentioning

confidence: 99%

Halotolerant rhizobacteria Pseudomonas pseudoalcaligenes and Bacillus subtilis mediate systemic tolerance in hydroponically grown soybean (Glycine max L.) against salinity stress

et al. 2020

View full text Add to dashboard Cite

Salt stress is one of the devastating factors that hampers growth and productivity of soybean. Use of Pseudomonas pseudoalcaligenes to improve salt tolerance in soybean has not been thoroughly explored yet. Therefore, we observed the response of hydroponically grown soybean plants, inoculated with halotolerant P. pseudoalcaligenes (SRM-16) and Bacillus subtilis (SRM-3) under salt stress. In vitro testing of 44 bacterial isolates revealed that four isolates showed high salt tolerance. Among them, B. subtilis and P. pseudoalcaligenes showed ACC deaminase activity, siderophore and indole acetic acid (IAA) production and were selected for the current study. We determined that 10 6 cells/mL of B. subtilis and P. pseudoalcaligenes was sufficient to induce tolerance in soybean against salinity stress (100 mM NaCl) in hydroponics by enhancing plant biomass, relative water content and osmolytes. Upon exposure of salinity stress, P. pseudoalcaligenes inoculated soybean plants showed tolerance by the increased activities of defense related system such as ion transport, antioxidant enzymes, proline and MDA content in shoots and roots. The Na + concentration in the soybean plants was increased in the salt stress; while, bacterial priming significantly reduced the Na + concentration in the salt stressed soybean plants. However, the antagonistic results were observed for K + concentration. Additionally, soybean primed with P. pseudoalcaligenes and exposed to 100 mM NaCl showed a new protein band of 28 kDa suggesting that P. pseudoalcaligenes effectively reduced salt stress. Our results showed that salinity tolerance was more pronounced in P. pseudoalcaligenes as compared to B. subtilis. However, a detailed study at molecular level to interpret the mechanism by which P. pseudoalcaligenes alleviates salt stress in soybean plants need to be explored.

show abstract

Section: Introductionmentioning

confidence: 99%

Halotolerant rhizobacteria Pseudomonas pseudoalcaligenes and Bacillus subtilis mediate systemic tolerance in hydroponically grown soybean (Glycine max L.) against salinity stress

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In addition, Burkholderia is considered a rhizospheric-plant promoting group ( Zhao K. et al, 2014 ). It could play an important role in maintaining the stability of soil communities ( Wakelin et al, 2017 ). The results of RDA suggested that the diazotrophic microbial community may respond differently to environmental parameters, but this hypothesis should be further tested.…”

Section: Discussionmentioning

confidence: 99%

nifH Gene Sequencing Reveals the Effects of Successive Monoculture on the Soil Diazotrophic Microbial Community in Casuarina equisetifolia Plantations

Zhou

Pokhrel

et al. 2021

Front. Plant Sci.

View full text Add to dashboard Cite

The growth and productivity of Casuarina equisetifolia is negatively impacted by planting sickness under long-term monoculture regimes. In this study, Illumina MiSeq sequencing targeting nifH genes was used to assess variations in the rhizospheric soil diazotrophic community under long-term monoculture rotations. Principal component analysis and unweighted pair-group method with arithmetic means (UPGMA) clustering demonstrated distinct differences in diazotrophic community structure between uncultivated soil (CK), the first rotation plantation (FCP), the second rotation plantation (SCP), and the third rotation plantation (TCP). Taxonomic analysis showed that the phyla Proteobacteria increased while Verrucomicrobia decreased under the consecutive monoculture (SCP and TCP). The relative abundance of Paraburkholderia, Rhodopseudomonas, Bradyrhizobium, Geobacter, Pseudodesulfovibrio, and Frankia increased significantly while Burkholderia, Rubrivivax, and Chlorobaculum declined significantly at the genus level under consecutive monoculture (SCP and TCP). Redundancy analysis (RDA) showed that Burkholderia, Rubrivivax, and Chlorobaculum were positively correlated with total nitrogen and available nitrogen. In conclusion, continuous C. equisetifolia monoculture could change the structure of diazotrophic microbes in the rhizosphere, resulting in the imbalance of the diazotrophic bacteria population, which might be a crucial factor related to replanting disease in this cultivated tree species.

show abstract

“…We isolated Pseudomonas sp. strain C9 during a study to identify novel bacterial strains that could be used to improve efficiency in nutrient use ( 3 ). The strain was isolated from the roots of Brassica oleracea L. (kale, cv.…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of a Kale ( Brassica oleracea L.) Root Endophyte, Pseudomonas sp. Strain C9

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

Isolation of root-associated Pseudomonas and Burkholderia spp. with biocontrol and plant growth-promoting traits

Cited by 9 publications

References 9 publications

Halotolerant rhizobacteria Pseudomonas pseudoalcaligenes and Bacillus subtilis mediate systemic tolerance in hydroponically grown soybean (Glycine max L.) against salinity stress

Halotolerant rhizobacteria Pseudomonas pseudoalcaligenes and Bacillus subtilis mediate systemic tolerance in hydroponically grown soybean (Glycine max L.) against salinity stress

nifH Gene Sequencing Reveals the Effects of Successive Monoculture on the Soil Diazotrophic Microbial Community in Casuarina equisetifolia Plantations

Draft Genome Sequence of a Kale ( Brassica oleracea L.) Root Endophyte, Pseudomonas sp. Strain C9

Contact Info

Product

Resources

About