Isolation of S-adenosyl-L-methionine synthetase gene from Panax ginseng C.A. meyer and analysis of its response to abiotic stresses

Pulla, Rama Krishna; Kim, Yujin; Parvin, Shahnaj; Shim, Ju-Sun; Lee, Junghye; Kim, Yeon-Ju; In, Jun-Gyo; Senthil, Kalaiselvi; Yang, Deok‐Chun

doi:10.1007/s12298-009-0030-x

Cited by 25 publications

(16 citation statements)

References 31 publications

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“…The only active methyl cycle enzyme not identified in the hypersalinity dataset was METsyn whose gel resolution was limited by its large size. Previous studies have also documented salinity-induced increases in SAMsyn genes Pulla et al, 2009). SAMsyn is responsible for the regeneration of SAM, the required cofactor for the penultimate methyltransferase reaction in DMSP synthesis.…”

Section: Candidate Dmsp Synthesis Enzymesmentioning

confidence: 89%

Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway

et al. 2011

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Dimethylsulfoniopropionate (DMSP) plays important roles in oceanic carbon and sulfur cycling and may significantly impact climate. It is a biomolecule synthesized from the methionine (Met) pathway and proposed to serve various physiological functions to aid in environmental stress adaptation through its compatible solute, cryoprotectant, and antioxidant properties. Yet, the enzymes and mechanisms regulating DMSP production are poorly understood. This study utilized a proteomics approach to investigate protein changes associated with salinity-induced DMSP increases in the model sea-ice diatom Fragilariopsis cylindrus (CCMP 1102). We hypothesized proteins associated with the Met-DMSP biosynthesis pathway would increase in relative abundance when challenged with elevated salinity. To test this hypothesis axenic log-phase cultures initially grown at a salinity of 35 were gradually shifted to a final salinity of 70 over a 24-h period. Intracellular DMSP was measured and two-dimensional gel electrophoresis was used to identify protein changes at 48 h after the shift. Intracellular DMSP increased by approximately 85% in the hypersaline cultures. One-third of the proteins increased under high salinity were associated with amino acid pathways. Three protein isoforms of S-adenosylhomo-cysteine hydrolase, which synthesizes a Met precursor, increased 1.8-to 2.1-fold, two isoforms of S-adenosyl Met synthetase increased 1.9-to 2.5-fold, and S-adenosyl Met methyltransferase increased by 2.8-fold, suggesting active methyl cycle proteins are recruited in the synthesis of DMSP. Proteins from the four enzyme classes of the proposed algal Met transaminase DMSP pathway were among the elevated proteins, supporting our hypothesis and providing candidate genes for future characterization studies.

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Section: Candidate Dmsp Synthesis Enzymesmentioning

confidence: 89%

Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway

et al. 2011

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“…Members of this family of protein are also regulated by stress-responsive TFs (Chen et al 2006 ). Pulla et al ( 2009 ) reported that S -adenosyl-L -methionine synthetase (SAMS) gene of Panax ginseng ( PgSAM ) expressed under various abiotic stresses in Panax ginseng and might be providing protection against environmental stresses.…”

Section: Gene Expression and Regulation In Response To Abiotic Stressesmentioning

confidence: 99%

Towards Understanding Abiotic Stress Signaling in Plants: Convergence of Genomic, Transcriptomic, Proteomic, and Metabolomic Approaches

Soni

Nutan

Soda

et al. 2015

Elucidation of Abiotic Stress Signaling in Plants

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All aspects of a plant's life-beginning with the seed germination and ending with the seed formation-are adversely affected by different abiotic stresses such as salinity, fl ood, drought, heat, cold, etc. Being sessile, plants have developed excellent mechanisms of stress perception and signal transduction. Multiple, complex, and dynamically intertwined interactions among nucleic acids, proteins, and metabolites determine the phenotype and fi nal response of plants towards environmental stresses. In response to these stresses, a multitude of processes are activated which enable the plants to cope with these stresses up to a certain extent. These include alteration of expression of stress-responsive genes, production of stress proteins, alteration of ion transport, activation of various antioxidant systems, and compatible solute accumulation. Our knowledge of abiotic stress signaling has grown in leaps and bounds since the emergence and developments in the omics technologies. Genome-scale studies at transcript, protein, and metabolite levels provide information about dynamic changes taking place at these functional levels. For full understanding of signaling networks, it is essentially important to integrate all these aspects. This approach is of remarkable applicability when the aim is to understand how plants react to abiotic stresses. In order to understand molecular basis of stress tolerance along with signaling network under unfavorable environmental situations, recent progress on systematic use of omics technologies including genomics, transcriptomics, proteomics, and metabolomics has been summarized in this chapter.

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“…PcSAMS1 is specific to root growth and formation, while PcSAMS2 functions more generally during plant growth (Lindroth et al 2001). SAMS from Panax ginseng was significantly under salt, salicylic acid, abscisic acid and chilling stresses (Pulla et al 2009). PySAMS gene isolated from Porphyra yezoensis was known to be stress related gene.…”

Section: Amino Acid Sequence Analysis and Alignmentmentioning

confidence: 99%

Characterization of S-adenosylmethionine Synthetase Gene from Red Algae, Pyropia tenera

Han

Hong

Han

et al. 2016

Plant Breed. Biotech.

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S-adenosylmethionine (SAM) is widely involved in variety of biosynthetic processes. It participates in the methionine (Met) metabolic pathway. SAM synthetase (EC 2.5.1.6) is the main enzyme that creates SAM from Met and adenosine triphosphate. SAMS genes are highly conserved in different species. We have isolated SAMS genes from red algae, Pyropia tenera. It has been reported that SAMS genes can confer abiotic stress-related response and growth of plant organs in other species. To elucidate the function of PtSAMS gene in evolutionary perspective, we analyzed SAMS gene using bioinformatics tools and plant transformation of Arabidopsis.

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Isolation of S-adenosyl-L-methionine synthetase gene from Panax ginseng C.A. meyer and analysis of its response to abiotic stresses

Cited by 25 publications

References 31 publications

Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway

Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway

Towards Understanding Abiotic Stress Signaling in Plants: Convergence of Genomic, Transcriptomic, Proteomic, and Metabolomic Approaches

Characterization of S-adenosylmethionine Synthetase Gene from Red Algae, Pyropia tenera

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