Isolation of Salmonella species and some other gram negative bacteria from rats cohabitating with poultry in Ibadan, Oyo State, Nigeria

Jemilehin, F O; Ogunleye, Ayodele A; Okunlade, A O; Ajuwape, A.T.P.

doi:10.5897/ajmr2015.7774

Cited by 7 publications

(2 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our records 5.00, 3.33, 1.66 and 1.66% for O. anthropi , Aeromonas sp., Micrococcus sp. and Morganella sp., respectively, are in accordance with many authors who stated the role of rat in contaminating the environment and dispersing diseases [ 20 , 96 , 101 , 102 ]. Nosocomial infections through dialysis fluids and contaminated hospital water supplies, mostly by carrier rats, suggest a good indicator for bacterial pollution of fresh water and promote spread of nosocomial infection [ 87 - 89 , 102 ].…”

Section: Discussionsupporting

confidence: 91%

Bacterial isolation from internal organs of rats (Rattus rattus) captured in Baghdad city of Iraq

et al. 2019

View full text Add to dashboard Cite

Aim: Rats are accused in disseminating many zoonotic diseases. This study aimed to isolate and identify bacteria from internal organs of rats captured in Baghdad City, Iraq. Materials and Methods: A total of 120 black rats (R. rattus) were trapped from different areas in Baghdad city. Rats were kept in individual plastic cages for 3 h before euthanizing. Deep pharyngeal swab, intestinal content, urine, and pieces of the liver and spleen, lung, kidney, and brain were obtained aseptically. The specimens were inoculated into peptone water and incubated at 37°C for 24 h for enrichment. A loopful of each specimen was then subcultured onto MacConkey Agar, Blood Agar, and Mannitol Salt Agar. CHROMagar O157 H7 and CHROMagar Listeria were used to detect Escherichia coli 157:7 and Listeria spp., respectively. Biochemical tests on analytical profile index, microscopic examination, and commercial kit for latex agglutination test for serotyping E. coli O157:H7 were used. Results: Mixed bacterial isolates were recorded as 116, 52, 36, 28, 18, 6, and 4 from intestinal contents, deep pharyngeal, liver and spleen, urine, lung, brain, and kidney, respectively. Microorganisms included E. coli, Staphylococcus aureus, Streptococcus spp., Bacillus spp., Pseudomonas aeruginosa, Citrobacter freundii, Proteus vulgaris, E. coli O157:H7, Enterobacter cloacae, Listeria spp., Klebsiella spp., Ochrobactrum anthropi, Aeromonas spp., Brucella spp., Pseudomonas fluorescens, Escherichia fergusonii, Micrococcus spp., Morganella spp., Proteus mirabilis, Pseudomonas luteola, and Streptobacillus spp. The highest bacterial prevalence (88; 73.33%) was recorded for E. coli, where 68 isolates were identified from the intestinal contents. Of these, four isolates were E. coli O157:H7. Conclusion: Rats are important carriers and transmitters of a number of pathogens and can disseminate these microorganisms to humans and animals.

show abstract

Section: Discussionsupporting

confidence: 91%

Bacterial isolation from internal organs of rats (Rattus rattus) captured in Baghdad city of Iraq

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Ochrobactrum intermedium has been frequently isolated from cold-and warm-blooded animals including poultry (Alonso, Kwabugge, Anyanwu, Torres, & Chah, 2017;Aujoulat et al, 2014;Bharucha, Sharma, Sharma, Kandil, & Collier, 2016;Sharma et al, 2018). Similarly, H. alvei has been previously isolated from variety of poultry sources including laying hens, pullets, broiler cecal contents, poultry meat products, air samples collected from poultry processing plants, and from rats and lizards cohabitating with poultry (Ajayi, Ogunleye, Happi, & Okunlade, 2015;Hinton, Buhr, & Ingram, 2000;Jemilehin, Ogunleye, Okunlade, & Ajuwape, 2016;Jones, Savell, Acuff, & Vanderzant, 1988;Lenhart, Olenchock, & Cole, 2009;Real et al, 1997;Vazquez-Moreno et al, 2006).…”

Section: Other Mdr Gram-negative Bacteriamentioning

confidence: 99%

The occurrence of Salmonella, extended‐spectrum β‐lactamase producing Escherichia coli and carbapenem resistant non‐fermenting Gram‐negative bacteria in a backyard poultry flock environment

Shah

Board

Crespo

et al. 2020

Zoonoses and Public Health

View full text Add to dashboard Cite

Increase in the number of small‐scale backyard poultry flocks in the USA has substantially increased human‐to‐live poultry contact, leading to increased public health risks of the transmission of multi‐drug resistant (MDR) zoonotic and food‐borne bacteria. The objective of this study was to detect the occurrence of Salmonella and MDR Gram‐negative bacteria (GNB) in the backyard poultry flock environment. A total of 34 backyard poultry flocks in Washington State (WA) were sampled. From each flock, one composite coop sample and three drag swabs from nest floor, waterer‐feeder, and a random site with visible faecal smearing, respectively, were collected. The samples were processed for isolation of Salmonella and other fermenting and non‐fermenting GNB under ceftiofur selection. Each isolate was identified to species level using MALDI‐TOFF and tested for resistance against 16 antibiotics belonging to eight antibiotic classes. Salmonella serovar 1,4,[5],12:i:‐ was isolated from one (3%) out of 34 flocks. Additionally, a total of 133 ceftiofur resistant (CefR) GNB including Escherichia coli (53), Acinetobacter spp. (45), Pseudomonas spp. (22), Achromobacter spp. (8), Bordetella trematum (1), Hafnia alvei (1), Ochrobactrum intermedium (1), Raoultella ornithinolytica (1), and Stenotrophomonas maltophilia (1) were isolated. Of these, 110 (82%) isolates displayed MDR. Each flock was found positive for the presence of one or more CefR GNB. Several MDR E. coli (n = 15) were identified as extended‐spectrum β‐lactamase (ESBL) positive. Carbapenem resistance was detected in non‐fermenting GNB including Acinetobacter spp. (n = 20), Pseudomonas spp. (n = 11) and Stenotrophomonas maltophila (n = 1). ESBL positive E. coli and carbapenem resistant non‐fermenting GNB are widespread in the backyard poultry flock environment in WA State. These GNB are known to cause opportunistic infections, especially in immunocompromised hosts. Better understanding of the ecology and epidemiology of these GNB in the backyard poultry flock settings is needed to identify potential risks of transmission to people in proximity.

show abstract

Exploration of the optimized parameters for bioactive prodigiosin mass production and its biomedical applications in vitro as well as in silico

Balasubramaniam¹,

Alexpandi

Darjily³

2019

Biocatalysis and Agricultural Biotechnology

View full text Add to dashboard Cite

Isolation of Salmonella species and some other gram negative bacteria from rats cohabitating with poultry in Ibadan, Oyo State, Nigeria

Abstract: The aim of this study was to isolate Salmonella species and other enteric bacteria in rats cohabiting with poultry; in order to consider the potential role of rats in their transmissions to poultry and humans.

Cited by 7 publications

References 27 publications

Bacterial isolation from internal organs of rats (Rattus rattus) captured in Baghdad city of Iraq

Bacterial isolation from internal organs of rats (Rattus rattus) captured in Baghdad city of Iraq

The occurrence of Salmonella, extended‐spectrum β‐lactamase producing Escherichia coli and carbapenem resistant non‐fermenting Gram‐negative bacteria in a backyard poultry flock environment

Exploration of the optimized parameters for bioactive prodigiosin mass production and its biomedical applications in vitro as well as in silico

Contact Info

Product

Resources

About