Isolation of Separate <i>Ureaplasma</i> Species From Endotracheal Secretions of Twin Patients

Beeton, Michael L.; Maxwell, Nicola C.; Chalker, Victoria J.; Brown, Rebecca J.; Aboklaish, Ali F.; Spiller, O. Brad

doi:10.1542/peds.2016-0565

Cited by 5 publications

(4 citation statements)

References 19 publications

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“…When examining the MIC values between the Ureaplasma spp., we noted that U. urealyticum had consistently higher MIC values at the CLSI recommended inoculum of 10 4 to 10 5 when compared with U. parvum. Although this was not a statistically significant difference, this reflects the observations in species difference seen when examining the activity of antibiotics against these pathogens (Beeton et al 2016).…”

Section: Resultsmentioning

confidence: 74%

“…Although this was not a statistically significant difference, this reflects the observations in species difference seen when examining the activity of antibiotics against these pathogens (Beeton et al . ). Of clinical relevance was the observation that bacterial load played a substantial role in the MIC for both U. parvum and U. urealyticum .…”

Section: Resultsmentioning

confidence: 97%

“…Of clinical relevance was the observation that bacterial load played a substantial role in the MIC for both U. parvum and U. urealyticum. Low-grade infections would be treatable with much lower concentrations of honey, where as those with high titres, as seen clinically, would require much higher concentrations (Beeton et al 2016). Antibiotic-resistant strains have been reported for the major classes of antibiotics effective against ureaplasmas, most notably the macrolides, tetracyclines and fluoroquinolones (Beeton et al 2009b(Beeton et al , 2015.…”

Section: Resultsmentioning

confidence: 99%

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Antimicrobial activity of Manuka honey against antibiotic-resistant strains of the cell wall-free bacteriaUreaplasma parvumandUreaplasma urealyticum

Hillitt

Jenkins

Spiller

et al. 2017

Lett Appl Microbiol

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The susceptibility of the cell wall‐free bacterial pathogens Ureaplasma spp. to Manuka honey was examined. The minimum inhibitory concentration (MIC) of Manuka honey for four Ureaplasma urealyticum and four Ureaplasma parvum isolates was determined. Sensitivity to honey was also compared to clinical isolates with resistance to tetracycline, macrolide and fluoroquinolone antibiotics. Finally step‐wise resistance training was utilized in an attempt to induce increased tolerance to honey. The MIC was dependent on the initial bacterial load with 7·5 and 18·0% w/v honey required to inhibit U. urealyticum at 1 and 106 colour changing units (CCU), respectively, and 4·8 and 15·3% w/v required to inhibit U. parvum at 1 and 106 CCU respectively. MIC values were consistently lower for U. parvum compared with U. urealyticum. Antimicrobial activity was seen against tetracycline‐resistant, erythromycin‐resistant and ciprofloxacin‐resistant isolates at 105 CCU. No resistance to honey was observed with 50 consecutive challenges at increasing concentrations of honey. This is the first report of the antimicrobial activity of Manuka honey against a cell wall‐free bacterial pathogen. The antimicrobial activity was retained against antibiotic‐resistant strains and it was not possible to generate resistant mutants. Significance and Impact of the Study Manuka honey is known to have a broad spectrum of antimicrobial activity, with the bacterial cell wall being suggested as a predominant site of action. This study has demonstrated that Manuka honey has activity against Ureaplasma spp., a genus of cell wall‐free bacteria which are intrinsically resistant to many available antibiotics making treatment inherently difficult. This is the first report of the antimicrobial activity of Manuka honey against a bacterial pathogen, in the absence of a cell well and opens scope for the use of components of Manuka honey as a therapeutic among Ureaplasma infections.

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Section: Resultsmentioning

confidence: 74%

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

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Antimicrobial activity of Manuka honey against antibiotic-resistant strains of the cell wall-free bacteriaUreaplasma parvumandUreaplasma urealyticum

Hillitt

Jenkins

Spiller

et al. 2017

Lett Appl Microbiol

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“…At present, data on quantitative amounts of Ureaplasma in infected neonates or pregnant women are scarce. 42,49,50 It can be speculated that knowledge of the actual bacterial load in a patient may correlate with clinical symptoms and for example could distinguish Ureaplasma carriage from infection.…”

Section: How To Diagnose Ureaplasma Infection?mentioning

confidence: 99%

Perinatal Infections With Ureaplasma

Stol¹,

Jans

Bruin

et al. 2021

Pediatric Infectious Disease Journal

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Ureaplasma species are increasingly recognized as relevant pathogens in prenatal, perinatal and postnatal morbidity. They are commonly found as commensals on the mucous membranes of the lower urogenital tract of pregnant women, but when ascending, they can cause bacterial vaginosis, chorioamnionitis, premature birth and postnatal morbidities such as bronchopulmonary dysplasia, and early-onset neonatal sepsis and meningitis. The detection of Ureaplasma species is challenging and is not covered by routine diagnostics, and current empiric antibiotic treatment in neonates suspected of infection is not directed against Ureaplasma species. The aim of this review is to discuss the pathophysiology of Ureaplasma infections, the clinical consequences and the current difficulties in diagnosis and treatment by providing an overview of the current literature.

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Differential recognition of the multiple banded antigen isoforms across Ureaplasma parvum and Ureaplasma urealyticum species by monoclonal antibodies

Aboklaish

Ahmed

McAllister³

et al. 2016

Journal of Microbiological Methods

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Two separate species of Ureaplasma have been identified that infect humans: Ureaplasma parvum and Ureaplasma urealyticum. Most notably, these bacteria lack a cell wall and are the leading infectious organism associated with infection-related induction of preterm birth. Fourteen separate representative prototype bacterial strains, called serovars, are largely differentiated by the sequence of repeating units in the C-terminus of the major surface protein: multiple-banded antigen (MBA). Monoclonal antibodies that recognise single or small groups of serovars have been previously reported, but these reagents remain sequestered in individual research laboratories. Here we characterise a panel of commercially available monoclonal antibodies raised against the MBA and describe the first monoclonal antibody that cross-reacts by immunoblot with all serovars of U. parvum and U. urealyticum species. We also describe a recombinant MBA expressed by Escherichia coli which facilitated further characterisation by immunoblot and demonstrate immunohistochemistry of paraffin-embedded antigens. Immunoblot reactivity was validated against well characterised previously published monoclonal antibodies and individual commercial antibodies were found to recognise all U. parvum strains, only serovars 3 and 14 or only serovars 1 and 6, or all strains belonging to U. parvum and U. urealyticum. MBA mass was highly variable between strains, consistent with variation in the number of C-terminal repeats between strains. Antibody characterisation will enable future investigations to correlate severity of pathogenicity to MBA isoform number or mass, in addition to development of antibody-based diagnostics that will detect infection by all Ureaplasma species or alternately be able to differentiate between U. parvum, U. urealyticum or mixed infections.

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Isolation of Separate Ureaplasma Species From Endotracheal Secretions of Twin Patients

Cited by 5 publications

References 19 publications

Antimicrobial activity of Manuka honey against antibiotic-resistant strains of the cell wall-free bacteriaUreaplasma parvumandUreaplasma urealyticum

Antimicrobial activity of Manuka honey against antibiotic-resistant strains of the cell wall-free bacteriaUreaplasma parvumandUreaplasma urealyticum

Perinatal Infections With Ureaplasma

Differential recognition of the multiple banded antigen isoforms across Ureaplasma parvum and Ureaplasma urealyticum species by monoclonal antibodies

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