Isolation of serotonin-O-sulfate from human urine

Davis, Virginia E.; Huff, James A.; Brown, Harold

doi:10.1016/0009-8981(66)90218-x

Cited by 13 publications

(6 citation statements)

References 3 publications

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“…The glucuronide of 5-HLAA was not found in sheep urine (Bartlet & Gilbert, 1970), however, indicating that little 5-HT-O-glucuronide was deaminated by monoamine oxidase in vivo. 5-HT-O-Sulphate may be eluted from an anion exchange resin with acetic acid (Davis, Huff & Brown, 1966). However, incubation with aryl sulphatase did not liberate 5-HT in the effluent from a column of Dowex-I (acetate form) loaded with sheep urine, although p-nitrocatechol sulphate added to the incubates was broken down completely with the liberation of p-nitrocatechol; thus sheep urine does not seem to contain 5-HT-O-sulphate.…”

mentioning

confidence: 93%

Estimation of urinary 5‐hydroxytryptamine‐O‐glucuronide, a metabolite of endogenous 5‐hydroxytryptamine in sheep

Bartlet¹,

Gilbert²

1971

British J Pharmacology

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confidence: 93%

Estimation of urinary 5‐hydroxytryptamine‐O‐glucuronide, a metabolite of endogenous 5‐hydroxytryptamine in sheep

Bartlet¹,

Gilbert²

1971

British J Pharmacology

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“…1983). The sulfated forms of these monoamines have been detected in blood, urine, and cerebrospinal fluids (Davis et al. 1966; Korf and Sebens 1970; Costa et al.…”

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confidence: 99%

“…Numerous studies using both animals and human subjects have demonstrated that sulfate conjugation of serotonin and dopamine plays an important role in the regulation and biotransformation of these monoamine neurotransmitters (Renskers et al 1980;Costa et al 1983;Reiter et al 1983). The sulfated forms of these monoamines have been detected in blood, urine, and cerebrospinal fluids (Davis et al 1966;Korf and Sebens 1970;Costa et al 1983;Tyce et al 1986). Studies have shown that abnormal levels and/or conjugation states of these monoamines may result in neurological disorders (Jones et al 1995;Stokes et al 1999).…”

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Hydroxylated serotonin and dopamine as substrates and inhibitors for human cytosolic SULT1A3

Yasuda

Liu

Suiko

et al. 2007

Journal of Neurochemistry

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Sulfation as catalyzed by the cytosolic sulfotransferases (SULTs) is known to play an important role in the regulation and homeostasis of monoamine neurotransmitters. The current study was designed to examine the occurrence of the sulfation of 7-hydroxyserotonin and 6-hydroxydopamine by human cytosolic SULTs and to investigate the inhibitory effects of these hydroxylated derivatives on the sulfation of their unhydroxylated counterparts, serotonin and dopamine. A systematic study using 11 known human cytosolic SULTs revealed SULT1A3 as the responsible enzyme for the sulfation of 7-hydroxyserotonin and 6-hydroxydopamine. The pH-dependence and kinetic constants of SULT1A3 with 7-hydroxyserotonin or 6-hydroxydopamine as substrate were determined. The inhibitory effects of 7-hydroxyserotonin and 6-hydroxydopamine on the sulfation of serotonin and dopamine were evaluated. Kinetic analyses indicated that the mechanism underlying the inhibition by these hydroxylated monoamine derivatives is of a competitive-type. 7-hydroxyserotonin or 6-hydroxydopamine. Upon transfection of the cells with siRNAs targeted at SULT1A3, diminishment of the SULT1A3 protein and concomitantly the sulfating activity toward these hydroxylated monoamines was observed. Taken together, these results indicated clearly the involvement of sulfation in the metabolism of 7-hydroxyserotonin and 6-hydroxydopamine. By serving as substrates for SULT1A3, these hydroxylated monoamines may interfere with the homeostasis of endogenous serotonin and dopamine.

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“…Laboratory test animals have been shown to excrete appreciable amounts of serotonin-O-sulfate after the administration of large doses of serotonin. However, the presence of this serotonin metabolite in the urine of normal man given oral loads of serotonin was not detected, but in the urine of patients with carcinoid tumors it has been quantified by utilizing ion exchange resins (Davis et al, 1966). So, the findings with urine showed lack of 5-HT-O-SO4 under physiological circumstances, probably due to insensitiveness of the methods employed.…”

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confidence: 99%

Quantification of serotonin O-sulphate by LC-MS method in plasma of healthy volunteers

Lozda

Purvins

2014

Front. Pharmacol.

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The objective of this study was to test the hypothesis that serotonin O-sulphate (5-HT-SO4) could be quantified in human plasma using modern liquid chromatography–mass spectrometry (LC-MS) method as well as develop and validate that method. First, a suitable LC-MS method for detection of 5-HT-SO4 in human plasma samples was developed and validated. Second, a Pilot phase involving four healthy volunteers was executed, where a basal plasma level of 5-HT-SO4 was measured for all subjects and for one after the intake of 100 mg of a 5-hydroxytryptophan (5-HTP) -containing food supplement used to promote serotonergic stimulation of the central nervous system. The basal level of 0.9–2.8 ng/mL of 5-HT-SO4 was observed. The changes of plasma 5HT-O-SO4 showed 1.2 ng/mL before and 22.6 ng/mL 1 h after stimulation. Finally, nine healthy volunteers were selected for the Study phase, where a basal plasma level of 5-HT-SO4 was measured before and after the intake of 5-HTP. One hour after stimulation, six study subjects showed a decrease in 5-HT-SO4 levels while three subjects showed an increase. The changes of plasma 5HT-O-SO4 from the Study phase showed an average 5-HT-SO4 level of 19.2 ng/mL before and 15.7 ng/mL 1 h after stimulation indicating ability of method to emphasize quantitative changes. This was the first study in which naturally occurring 5-HT-SO4 was detected in the samples of human plasma obtained from healthy volunteers. The method developed herein is specific to the measurement of 5-HT-SO4, sensitive enough to quantify intra-individual changes in the samples of plasma and opens up new possibilities to evaluate pathways of serotonin metabolism by minimally invasive methods. The discovery of novel biomarkers using such approaches is increasingly required to expedite development of mechanism-based therapeutics and patient stratification.

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Isolation of serotonin-O-sulfate from human urine

Cited by 13 publications

References 3 publications

Estimation of urinary 5‐hydroxytryptamine‐O‐glucuronide, a metabolite of endogenous 5‐hydroxytryptamine in sheep

Estimation of urinary 5‐hydroxytryptamine‐O‐glucuronide, a metabolite of endogenous 5‐hydroxytryptamine in sheep

Hydroxylated serotonin and dopamine as substrates and inhibitors for human cytosolic SULT1A3

Quantification of serotonin O-sulphate by LC-MS method in plasma of healthy volunteers

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