Isolation of Simian Virus 40 from Rhesus Monkeys (Macaca mulatta) with Spontaneous Progressive Multifocal Leukoencephalopathy

Holmberg, C. A.; Gribble, David H.; Takemoto, Kenneth K.; Howley, Peter M.; Espana, Carlos; Osburn, Bennie

doi:10.1093/infdis/136.4.593

Cited by 56 publications

(18 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The disease has a fatal outcome a few months or years after symptoms appear, and JCV can be seen by electron microscopy in oligodendrocytes (Gardner, 1977), The disease is usually found in persons who have impaired cell-mediated immunity as a result of some concurrent chronic disease or immunosuppressive therapy (ZuRhein, 1969). A similar disease has also been observed in monkeys in association with their papovavirus, SV40 (Holmberg et al, 1977). This paper reports the finding that polyoma virus can cause a paralytic disease in homozygous nude mice which have an impaired cell-mediated immune response.…”

Section: Discussionsupporting

confidence: 73%

“…Electron microscopy of the brains of the paralysed mice revealed areas of disruption and degeneration of the myelin sheaths, due perhaps to polyoma replication in oligodendrocytes (Fig. 1), although plaques of demyelination were not observed as in PML in humans (ZuRhein, 1969) and monkeys (Holmberg et al, 1977). It may be that plaques would have developed if the mice had been left longer, but all were harvested at the first sign of paralysis.…”

Section: Discussionmentioning

confidence: 97%

“…The condition was not unlike PML in man (ZuRhein, 1969) and in monkeys (Holmberg et al, 1977). The husbandry of the mice, epidemiological, pathological and electron microscope findings have been described elsewhere (Sebesteny et al, 1980).…”

Section: Introductionmentioning

confidence: 97%

See 2 more Smart Citations

A Paralytic Disease in Nude Mice Associated with Polyoma Virus Infection

McCance

Sebesteny

Griffin

et al. 1983

Journal of General Virology

View full text Add to dashboard Cite

SUMMARYNude mice (nu/nu), heterotransplanted with human tumours and kept in isolators, were found to suffer from wasting and posterior paralysis. Electron microscopy of spinal cord tissue revealed virus particles in tlae oligodendrocytes consistent in size (35 to 40 nm), morphology and distribution with those of the polyoma-SV40 sub-group of papovaviruses. Serology and restriction enzyme analysis of the virus genome showed that the virus was the murine polyoma A2 strain. Inoculation of uninfected nude mice with 107 TCID5o of polyoma A2 strain virus produced a similar disease in these mice with wasting and, after 10 to 23 weeks, paralysis of the hind legs of all surviving mice. Extensive myelin disruption was seen throughout the brain stem and sacral region of the spinal cord and high titres of polyoma virus were found in the whole brain (10 s'8 TCID5o/brain) and in the spinal cord (106.8 TCIDs0/spinal cord).

show abstract

Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

A Paralytic Disease in Nude Mice Associated with Polyoma Virus Infection

McCance

Sebesteny

Griffin

et al. 1983

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…SV40 is the causative agent of PML in macaques [32]; it induces PML in macaques affected by malignant diseases or chronic infections, with a relatively low incidence [33]. However, macaques with SIV-induced AIDS are highly predisposed to primary and recrudescent polyomaviral diseases [34].…”

Section: Introductionmentioning

confidence: 99%

Investigation of the simian polyomavirus SV40 as a potential causative agent of human neurological disorders in AIDS patients

Tognon

Martini

Iaccheri

et al. 2001

Journal of Medical Microbiology

View full text Add to dashboard Cite

Neurological diseases and a variety of neoplasms frequently occur in AIDS patients. Human JC and BK polyomaviruses have been associated with neurological disorders in such patients. SV40 polyomavirus sequences have been detected in human brain tumours, other neoplasms and normal tissues. JCV, BKV and SV40 DNA sequences were investigated in cerebrospinal¯uid (CSF) samples from 12 AIDS patients affected by different neurological disorders, by PCR assay and ®lter hybridisation with speci®c internal oligoprobes, and DNA sequencing. Three of the 12 CSF samples were positive for JCV (one sample) or SV40 (one) DNA, or both (one). No sample was positive for BKV DNA. JCV-and SV40-speci®c genomic regions were con®rmed by DNA sequencing. CSF samples from the two patients diagnosed clinically as having progressive multifocal leukoencephalopathy (PML) contained either JCV (one sample) or SV40 (one) DNA. The CSF found to contain both JCV and SV40 DNA originated from a patient with a cerebral mass lesion of unknown aetiology. These results suggest that SV40 may be involved in the aetiology of PML in AIDS patients, and raise the possibility that SV40 and JCV may act synergically in vivo to enhance their pathogenicity.

show abstract

“…As this polyclonal antiserum is not specific for JCV, but cross-reacts with SV40 -which is known to cause PML in the macaque [29] and, reportedly, in man [30] -a series of MAbs raised against SV40 VP1 was used to characterise hrther the antigenic nature of the infecting agent. Twelve antibodies that immunoprecipitate SV40 VP1 [31] were tested for reactivity on formalin-fixed, paraffin-embedded CNS sections from PML in the macaque brain.…”

Section: Pml In a Ugandan Aidspatient (Case 1)mentioning

confidence: 99%

Detection of JC virus in two African cases of progressive multifocal leukoencephalopathy including identification of JCV type 3 in a Gambian AIDS patient

Stoner¹,

Agostini²,

Ryschkewitsch³

et al. 1998

Journal of Medical Microbiology

View full text Add to dashboard Cite

Progressive multifocal leukoencephalopathy (PML) is a fatal demyelinating central nervous system (CNS) infection, affecting mainly oligodendrocytes, but also occasional astrocytes. In the USA, Europe and Asia, PML is caused by the human polyomavirus JC virus (JCV) and in autopsy series occurs in about 4-7% of AIDS patients. In Africa, the prevalence of PML in AIDS patients is uncertain and the causative agent is unknown. This study reports immunocytochemical and PCR confirmation of PML in the CNS of an AIDS patient dying in Uganda, East Africa (case 1). In a Gambian patient infected with HIV-2 who died 3 months after onset of AIDS/PML in Germany (case 2), it was possible to confirm the identity of the virus by DNA sequencing of the PCR amplified JCV product. This African genotype of the virus (type 3) showed an unusual re-arrangement of the regulatory region, and could be distinguished at several sites from East African and African-American JCV strains described previously. This study has confirmed that PML is a complication of African AIDS as it is in Europe and the USA, and that JCV type 3 is pathogenic in African AIDS patients. Furthermore, the finding of an African genotype of JCV in a patient dying in Germany suggests that in this individual JCV represented a latent infection acquired in Africa.

show abstract

Isolation of Simian Virus 40 from Rhesus Monkeys (Macaca mulatta) with Spontaneous Progressive Multifocal Leukoencephalopathy

Cited by 56 publications

References 14 publications

A Paralytic Disease in Nude Mice Associated with Polyoma Virus Infection

A Paralytic Disease in Nude Mice Associated with Polyoma Virus Infection

Investigation of the simian polyomavirus SV40 as a potential causative agent of human neurological disorders in AIDS patients

Detection of JC virus in two African cases of progressive multifocal leukoencephalopathy including identification of JCV type 3 in a Gambian AIDS patient

Contact Info

Product

Resources

About