Isolation of single osteons and osteon lamellae

Frasca, Peter; Harper, Richard A.; Katz, Lawrence

doi:10.1159/000144608

Cited by 17 publications

(6 citation statements)

References 2 publications

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“…Demineralized Samples Demineralisation of bone samples has been employed to permit interlamellar separation, thus making it easier to determine the preferred collagen bundle orientation in successive bone lamellae (Frasca et al, 1976). Archival demineralized bone histology material may also be investigated (Fig.…”

Section: Cells Retainedmentioning

confidence: 99%

Scanning electron microscopy of bone: Instrument, specimen, and issues

Boyde

Jones

1996

Microsc. Res. Tech.

103

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Section: Cells Retainedmentioning

confidence: 99%

Scanning electron microscopy of bone: Instrument, specimen, and issues

Boyde

Jones

1996

Microsc. Res. Tech.

103

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“…Part of the complexity arises from the size of the osteons and the inherent difficulty to their isolation. The methodology proposed in this paper seems to be less time consuming and as effective as the previous attempts by Ascenzi et al, (1959), (1967), (1985), (1994), Frasca et al, (1976), and Dong et al, (2005). Another component of the problem stems from the complexity of the analytical solution which requires the determination of both the matrix material properties and the drained elastic constants.…”

Section: Discussionmentioning

confidence: 71%

“…Ascenzi and coworkers (Ascenzi and Fabri 1959; Ascenzi and Bonucci 1967 Ascenzi et al, 1985; Ascenzi et al, 1994) developed a method for extraction of osteons from a thin cross section of bone using a sharp steel needle eccentrically inserted in a dentist’s drill or using a custom made micro lathe. Osteons have also been isolated by propagating a fracture through the natural boundary of the osteon (Frasca et al, 1976) and using a precision and computer controlled osteon pushout micro-testing system (Dong et al, 2005). …”

Section: Methodsmentioning

confidence: 99%

Experimental Determination of the Permeability in the Lacunar-Canalicular Porosity of Bone

Gailani

Benalla

Mahamud

et al. 2009

Journal of Biomechanical Engineering

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Permeability of the mineralized bone tissue is a critical element in understanding fluid flow occurring in the lacunar-canalicular porosity (PLC) compartment of bone and its role in bone nutrition and mechanotransduction. However, the estimation of bone permeability at the tissue level is affected by the influence of the vascular porosity (PV) in macroscopic samples containing several osteons. In this communication, both analytical and experimental approaches are proposed to estimate the lacunar-canalicular permeability in a single osteon. Data from an experimental stress-relaxation test in a single osteon is used to derive the PLC permeability by curve fitting to theoretical results from a compressible transverse isotropic poroelastic model of a porous annular disk under a ramp loading history (Cowin and Mehrabadi 2007;Gailani and Cowin 2008). The PLC tissue intrinsic permeability in the radial direction of the osteon was found to be dependent on the strain rate used and within the range of O(10 −24 )−O(10 −25 ). The reported values of PLC permeability are in reasonable agreement with previously reported values derived using FEA and nanoindentation approaches.

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“…Frasca et al . separated and imaged lamellae in scanning electron microscopy, however, they did not make a distinction between primary and secondary osteons, nor image sufficient length to determine long-range three-dimensional lamellar shape [ 69 ]. Mohsin et al .…”

Section: Lamellaementioning

confidence: 99%

Closing cones create conical lamellae in secondary osteonal bone

Doube

2022

R. Soc. open sci.

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Lamellae are sheets of mineralized collagen 1–20 µm thick, extending over hundreds of µm in bone tissue, occupying bone's structural hierarchy at a level above collagen fibres and osteocytes, and below osteons and trabeculae. Osteons are tubular arrangements of lamellae surrounding central neurovascular canals. Lamellae in osteons are usually described as concentric cylinders based on their annular appearance in transverse section. In this review, I provide a perspective on current understanding of the relationship between geometry of the bone formation front and the shape of lamellae produced at it, reaching the conclusion that the ‘closing cone’ bone formation front in secondary osteonal remodelling must necessarily result in cone-shaped lamellae in the mature secondary osteon. Secondary osteons replace primary osteons through a tunnelling process of bone turnover, meaning that conical lamellae may become more common in older and damaged bone which is at greatest risk of fracture. Visualization and measurement of three-dimensional lamellar shape over hundreds of microns is needed to provide data for accurate micromechanical simulations. Treating secondary osteonal lamellae as a ‘stack of cones’ rather than ‘nested cylinders’ may have important implications for our appreciation of bone's function as a load-bearing tissue and of its behaviour in fracture.

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Isolation of single osteons and osteon lamellae

Cited by 17 publications

References 2 publications

Scanning electron microscopy of bone: Instrument, specimen, and issues

Scanning electron microscopy of bone: Instrument, specimen, and issues

Experimental Determination of the Permeability in the Lacunar-Canalicular Porosity of Bone

Closing cones create conical lamellae in secondary osteonal bone

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