Isolation of small-sized human epidermal progenitor/stem cells by Gravity Assisted Cell Sorting (GACS)

Equine Epidermis: A Source of Epithelial-Like Stem/Progenitor Cells with In Vitro and In Vivo Regenerative Capacities

“…The isolation of skinderived precursor cells with neurogenic properties has been previously reported from human dermis [13][14][15] and hair follicles [16] as have the isolation and partial purification of adult multipotent stem cells from human and murine epidermis [17,18]. For instance, Fujimori et al reported the isolation of human epidermal stem/progenitor cells by means of gravity-assisted cell sorting [17]. However, the practice of selecting for stem cells based on size (diameter) is problematic given that there exists a significant proportion of somatic cells with equally small diameters, ultimately resulting in a heterogeneous yield of cell types.…”

In vitro study on proliferation kinetics of oral mucosal keratinocytes

“…9 Different methods of characterizing human epidermal stem cells, such as separation of stem cell populations by gravity assisted cell sorting (GACS), identification by expression of neurotrophin receptors p75, and isolation of stem cells by mean of marker proteins such as b1-integrin, are described in the literature. [17][18][19] Alternative techniques in stem cell isolation also achieved acceptable results but failed in concrete implementation; therefore, the major aim of this study was to develop a technique that allows rapid proliferation of keratinocytes for clinical use in oral mucosal scaffolds and that does not suffer from technical complexity and costly additions. The fast availability of full-thickness human autologous oral mucosa as tissue-engineered 3-D scaffolds would certainly simplify its in vivo use and extend its clinical applications.…”

Intraepidermal Injections of Autologous Epidermal Cell Suspension: A new promising approach to Dermatological Disorders. Preliminary Study

“…Cell morphology was evaluated before the seeding and after a 7 day culture by using an inverted Olympus microscope (Olympus Italia S.R.L., Milan, Italy). The protocols considered previously reported works [16][17][18][19][20].…”