Isolation of Staphylococcus aureus from Raw Cattle Milk and their Drug Resistance Pattern

Isolation, Characterization and Sensitivity Pattern of Staphylococcus aureus Isolated from Milk Samples in Khartoum State–Sudan

“…The S. aureus was detected in 42% of the total examined milk samples in Egypt (Awad et al, 2017). Lower percentage (10.16%) was reported in raw milk samples (Patel et al, 2018). The prevalence rate of Staphylococcus aurues shows variable variation ranging from 40% down to 10% (Patel et al, 2018) Several studies reported that Staphylococcus aureus is the main cause of mastitis (Kerro and Tareke, 2003;Hundera et al, 2005;Mekonnen et al, 2005).…”

“…Lower percentage (10.16%) was reported in raw milk samples (Patel et al, 2018). The prevalence rate of Staphylococcus aurues shows variable variation ranging from 40% down to 10% (Patel et al, 2018) Several studies reported that Staphylococcus aureus is the main cause of mastitis (Kerro and Tareke, 2003;Hundera et al, 2005;Mekonnen et al, 2005). A study carried by Abera et al, (2013) in Ethiopia, showed the percentage of Staphylococcus aureus isolated from mastitis cows was 42.1 % which renders it as one of the problem causing in dairy farms,…”

“…The variability in prevalence of mastitis results may be affected by different factors such as the season, farm management practices sampling procedures, method of isolation and hygienic practice of milking and selling (Abera et al, 2013;Lili et al, 2016;Patel et al, 2016). An important feature of Staphylococcus aurues is it that it usually survives in the udder resulting in clinical or sub clinical mastitis, but it also can shed into the milk and it becomes a source for infection to other healthy cows during milking-process and become a main source of contaminants (Patel et al, 2018) Regarding the sensitivity tests, the previously isolated and identified Staphylococcus aureus was tested against three commonly used antibiotics namely Penicillin, Sulpamethoxazole / Trimethoprim and Ciprofloxacine. The isolates were reported as sensitive, intermediate or resistant after measuring the inhibition zone around the specific disc and then classified according to (Chengappa, 1990;CLSI document 2008).All sample (47 isolates) were found to be resistant to Penicillin while susceptible to Sulpamethoxazole /Trimethoprim and Ciprofloxacine (Fig.…”

Pathogenic bacteria in cheese, raw and pasteurised milk

“…This is of great importance, especially with increasing use of antibiotics, which has increased the resistance to various drugs significantly and has made control and treatment in humans and animals more difficult. Therefore, rising antibiotic resistance is a concern and should be controlled (Patel et al., 2018 ). Milk is a good environment for S. aureus to grow and produce enterotoxin, especially because enterotoxin retains its biological activity even after pasteurisation.…”

Antimicrobial Resistance Properties of Staphylococcus Aureus Isolates From Powdered Packaged Medicinal Plants and Bottle Herbal Distillates

“…At that point, ve milliliters of the achieved solution was transferred into 50 mL Trypticase Soy Broth (TSB, Merck, Germany) supplemented with 10% NaCl and 1% sodium pyruvate and incubated for 18 h at 35 °C. At that moment, a loopful of the culture was transferred into Baird-Parker agar supplemented with egg yolk tellurite emulsion (Merck, Germany) and incubated at 37 °C for about 24 h. Black shiny colonies enclosed with signi cant zones identi ed using biochemical tests including Gram staining, oxidase test, catalase activity, resistance to bacitracin (0.04 U), coagulated test (rabbit plasma), urease activity, glucose O/F test, vogesproskaver (Merck, Germany) test, nitrate reduction, phosphatase, deoxyribonuclease (DNase, Merck, Germany) test, mannitol fermentation, hemolysis activity on blood agar (Merck, Germany) and carbohydrate (xylose, sucrose, trehalose and maltose, fructose, lactose, mannose) fermentation tests [19].…”