Isolation Of Stem Cells From The Mid Gut Epithelium Of Culex Pipiens Mosquitoes ( Diptera : Culicidae )

Wassim, Nahla M.; Soliman, Belal A.; Hussein, Mmm; Metwaly, Hala

doi:10.12816/0006440

Cited by 3 publications

(3 citation statements)

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“…Cells have spherical nuclei. Results of the present study were comparable to those found by Wassim et al (2014) working on Cx. pipiens midgut epithelium.…”

Section: Resultssupporting

confidence: 91%

Ultrastructural alterations in the midgut of Bacillus sphaericus-treated Culex pipiens (Diptera: Culicidae) larvae

Tewfick

Soliman

2018

Egypt J Biol Pest Control

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The ultrastructure alterations were described in the midgut of Culex pipiens (Diptera: Culicidae) larvae following Bacillus sphaericus treatment. Two hours post-treatment, the epithelial layer began to have large amount of vacuoles. Explosion in the basement membrane took place 6 h post-treatment. Ten hours post-treatment, the bacterium began to invade the microvilli. At 14 h post-bacterial treatment, epithelial cells were destroyed, microvilli were damaged, and bacteria were present in lumen in large amount. The present study provided the evidence on the main aberrations induced in midgut larvae of Cx. pipiens as a result of ingesting B. sphaericus.

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“…Cells have spherical nuclei. Results of the present study were comparable to those found by Wassim et al (2014) working on Cx. pipiens midgut epithelium.…”

Section: Resultssupporting

confidence: 91%

Ultrastructural alterations in the midgut of Bacillus sphaericus-treated Culex pipiens (Diptera: Culicidae) larvae

Tewfick

Soliman

2018

Egypt J Biol Pest Control

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“…As a first step, however, the identification of stem cell types in a variety of insects has been reported (Corley and Lavine, 2006). In the case of mosquitoes, midgut stem cells from the house mosquito Culex pipiens have been isolated and cultured, though for a limited period of time (Wassim et al, 2014). This pioneer work demonstrates that it is possible to obtain, culture and store insect stem cells, raising exciting possibilities for the generation of longerterm stem cells cultures capable of supporting a pathogen infection.…”

Section: Stem Cell Technologies and Organoidsmentioning

confidence: 99%

Novel systems to study vector-pathogen interactions in malaria

Parres-Mercader

Pance

Gómez-Díaz

2023

Front. Cell. Infect. Microbiol.

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Some parasitic diseases, such as malaria, require two hosts to complete their lifecycle: a human and an insect vector. Although most malaria research has focused on parasite development in the human host, the life cycle within the vector is critical for the propagation of the disease. The mosquito stage of the Plasmodium lifecycle represents a major demographic bottleneck, crucial for transmission blocking strategies. Furthermore, it is in the vector, where sexual recombination occurs generating “de novo” genetic diversity, which can favor the spread of drug resistance and hinder effective vaccine development. However, understanding of vector-parasite interactions is hampered by the lack of experimental systems that mimic the natural environment while allowing to control and standardize the complexity of the interactions. The breakthrough in stem cell technologies has provided new insights into human-pathogen interactions, but these advances have not been translated into insect models. Here, we review in vivo and in vitro systems that have been used so far to study malaria in the mosquito. We also highlight the relevance of single-cell technologies to progress understanding of these interactions with higher resolution and depth. Finally, we emphasize the necessity to develop robust and accessible ex vivo systems (tissues and organs) to enable investigation of the molecular mechanisms of parasite-vector interactions providing new targets for malaria control.

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“…However, their involvement in tissue repair seems to be influenced by various mechanisms which still need further investigations. The insect MG's epithelium can also contain some goblet cells too; but in mosquitoes, those cells seem to have a role during larval stage but not in adult stage [Wassim et al., 2014]. The purpose of those goblet cells in larvae is to reinforce the development of the mosquito into an adult stage (Figure 1B).…”

Section: In a Healthy Mgmentioning

confidence: 99%

Battleground midgut: The cost to the mosquito for hosting the malaria parasite

Hajkazemian

Bossé

Mozūraitis

et al. 2020

Biology of the Cell

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In eco‐evolutionary studies of parasite–host interactions, virulence is defined as a reduction in host fitness as a result of infection relative to an uninfected host. Pathogen virulence may either promote parasite transmission, when correlated with higher parasite replication rate, or decrease the transmission rate if the pathogen quickly kills the host. This evolutionary mechanism, referred to as ‘trade‐off’ theory, proposes that pathogen virulence evolves towards a level that most benefits the transmission. It has been generally predicted that pathogens evolve towards low virulence in their insect vectors, mainly due to the high dependence of parasite transmission on their vector survival. Therefore, the degree of virulence which malaria parasites impose on mosquito vectors may depend on several external and internal factors. Here, we review briefly (i) the role of mosquito in parasite development, with a particular focus on mosquito midgut as the battleground between Plasmodium and the mosquito host. We aim to point out (ii) the histology of the mosquito midgut epithelium and its role in host defence against parasite's countermeasures in the three main battle sites, namely (a) the lumen (microbiota and biochemical environment), (b) the peritrophic membrane (physical barrier) and (c) the tubular epithelium including the basal membrane (physical and biochemical barrier). Lastly, (iii) we describe the impact which malaria parasite and its virulence factors have on mosquito fitness.

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Isolation Of Stem Cells From The Mid Gut Epithelium Of Culex Pipiens Mosquitoes ( Diptera : Culicidae )

Cited by 3 publications

References 0 publications

Ultrastructural alterations in the midgut of Bacillus sphaericus-treated Culex pipiens (Diptera: Culicidae) larvae

Ultrastructural alterations in the midgut of Bacillus sphaericus-treated Culex pipiens (Diptera: Culicidae) larvae

Novel systems to study vector-pathogen interactions in malaria

Battleground midgut: The cost to the mosquito for hosting the malaria parasite

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