Isolation of strains of Thermus aquaticus from the Australian Artesian Basin and a simple and rapid procedure for the preparation of their plasmids

Denman, Stuart E.

doi:10.1016/0378-1097(91)90423-8

Cited by 16 publications

(13 citation statements)

References 8 publications

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“…Chemically, the water can be carbonate-, sulfate-and\or iron-rich. Previously, we have reported the isolation of a range of taxa of aerobic and anaerobic thermophilic species of the genera Thermus (Denman et al, 1991 ;Byers et al, 1997), Caloramator , Fervidobacterium (Andrews & Patel, 1996), Desulfotomaculum (Love et al, 1993), Thermoanaerobacter (Wynter et al, 1996) and Desulfovibrio (Redburn & Patel, 1994) and a great number of micro-organisms of unidentified taxa . In this report, we describe a novel mesophilic, facultatively anaerobic Bacillus species capable of reducing iron and manganese anaerobically.…”

Section: Introductionmentioning

confidence: 97%

Bacillus subterraneus sp. nov., an iron- and manganese-reducing bacterium from a deep subsurface Australian thermal aquifer

Kanso¹

2002

International Journal of Systematic and Evolutionary Microbiology

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A facultatively anaerobic bacterium, designated strain COOI3B(T) (= ATCC BAA 136T = DSM 13966T), was isolated from the waters emitted by a bore well tapping the deep subterranean thermal waters of the Great Artesian Basin of Australia. The cells were straight to slightly curved rods (0.5-0.8 x 2-25 microm) that occurred singly and rarely in pairs or in chains. Strain COOI3B(T) was motile by peritrichous flagella. It stained gram-negative, but electron micrographs showed a gram-positive-type cell wall. Spores were never observed and cells were heat-sensitive. Yeast extract at 0.02% (w/v) was required for growth and could also be used as a sole carbon and energy source at concentrations higher than 0.1% (w/v). The strain utilized amorphous iron(III), manganese(IV), nitrate, nitrite and fumarate as electron acceptors in the presence of yeast extract, glucose, sucrose, fructose, maltose, xylose, starch, glycerol, ethanol or lactate. Electron acceptors were not obligately required and growth was better in the presence of nitrate than in its absence. Acid was not produced from growth on carbohydrates. Tryptophan deaminase, H2S, arginine dihydrolase, lysine decarboxylase, beta-galactosidase, arabinosidase, glucuronidase, glucosaminidase, nitroanilidase, xylosidase and ornithine decarboxylase were not produced. Starch and gelatin, but not casein, were hydrolysed. Aesculin and catalase, but not oxidase and urease, were produced. Strain COOI3B(T) grew optimally at temperatures between 37 and 40 degrees C (the temperature growth range was 25-45 degrees C) and at pH 7.0-9.0 (the pH growth range was 6.0 to 9.5) with 5% (w/v) NaCl (the NaCl concentration growth range was 0.9%, w/v). The DNA base composition was 43 +/- 1 mol % G+C. Phylogenetic analysis indicated that it was a member of the family Bacillaceae, Bacillus infernus and Bacillus firmus being the closest phylogenetic neighbours (having a mean similarity value of 96%); hence, strain COOI3B(T) is designated as a novel species, Bacillus subterraneus sp. nov.

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Section: Introductionmentioning

confidence: 97%

Bacillus subterraneus sp. nov., an iron- and manganese-reducing bacterium from a deep subsurface Australian thermal aquifer

Kanso¹

2002

International Journal of Systematic and Evolutionary Microbiology

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“…Thermus and Meiothermus have so far been isolated from terrestrial and shallow marine hot springs of USA [1], Iceland [9], New Mexico [10], deep sea hydrothermal vents [11], New Zealand [12], Japan [13,14], Russia [15], shallow marine hot springs in Portugal [3,16] and from the subterranean aquifer, the Great Artesian Basin (GAB) of Australia [17,18]. Thermus species have also been isolated from man‐made environments such as composts [19] and hot water systems [20].…”

Section: Introductionmentioning

confidence: 99%

The phylogenetic diversity of Thermus and Meiothermus from microbial mats of an Australian subsurface aquifer runoff channel

Spanevello

Patel

2004

FEMS Microbiology Ecology

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Spectral analysis of the cell free extracts of four mat samples colonizing a Great Artesian Bain (GAB) aquifer bore runoff channel suggested that Thermus was present in the 75 degrees C grey mat, Meiothermus was present in the 66 degrees C red mat, a mixed population of Meiothermus/Thermus and photosynthetic microbes were present in the 57 degrees C green mat and photosynthetic microbes were present in the 52 degrees C brown mat. Enumeration studies indicated that Thermus dominated the grey mats and Meiothermus dominated the red mat but both were absent in samples collected at the bore source (89 degrees C) and below the bore source (88 degrees C). Culture-dependent studies followed by 16S rRNA gene sequence analysis indicated that 13 of the 14 Thermus isolates clustered closely with each other and to T. igniterrae, with the remaining strain clustering with Thermus strain SRI-96. The two Meiothermus isolates were closely related to Meiothermus ruber. A culture-independent study with 367 16S rRNA gene clones concurred that Thermus dominated the grey mat, but to a lesser extent in the red mat and the green mats and its complete absence in the brown mat. Of the four Thermus phylogroups identified one phylogroup dominated the cloned library and was related to the cluster represented by T. scotoductus. The second most dominant phylogroup was related to the cluster represented by T. igniterrae and the third and fourth phylogroups, which were the least dominant, were related to cluster represented by Thermus strain SRI-248 and T. oshima respectively. Meiothermus was only represented in the 16S rRNA gene libraries of the red, green and brown mats and formed two phylogroups, of which the most dominant was associated with the red mat and phylogenetically related to M. ruber while the second phylogroup was found only in the green mat gene library and was related to M. cerberus.

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“…Members of the genus Thermus have previously been isolated from a number of GAB bores [37], and appear to be present in the GAB mat. These aerobic heterotrophs utilise organic matter derived from autotrophic bacteria.…”

Section: Discussionmentioning

confidence: 99%

Molecular investigation of a microbial mat associated with the Great Artesian Basin

Byers¹,

Stackebrandt²,

Hayward³

et al. 1998

FEMS Microbiology Ecology

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Culture‐independent modes of analysis were chosen to investigate a microbial mat associated with the thermal waters of the Great Artesian Basin (GAB). 16S rDNA was amplified from total genomic mat DNA, and used to construct a clone library. Use of plasmid‐specific primers to amplify the inserts from 92 selected recombinants proved to be an effective approach, and demonstrated that there were four size categories of insert: 1500 bp (62% of clones examined), 1400 bp (25% of clones examined), 500 bp (5% of clones examined) and 240 bp (8% of clones examined). Restriction enzyme analysis was evaluated for its ability to group the 1500 bp and 1400 bp size inserts into operational taxonomic units. Clone inserts were presumptively identified by analysis of partial sequence data, and each operational taxonomic unit was found to be phylogenetically cohesive. Phylogenetic analyses of the sequence data indicated the presence of a broad range of bacteria related to the cyanobacteria, Thermus species, thiobacilli, planctomycetes, thermophilic hydrogen oxidisers, thermotogales, clostridia, actinomycetes, and β and δ subclasses of the proteobacteria. Use of the restriction enzyme analysis protocol also enabled the extent of insert repetition within the library to be monitored, and would thus have eliminated 70% of sequencing expenses. This is the first time a community analysis has been performed on this extreme environment.

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Isolation of strains of Thermus aquaticus from the Australian Artesian Basin and a simple and rapid procedure for the preparation of their plasmids

Cited by 16 publications

References 8 publications

Bacillus subterraneus sp. nov., an iron- and manganese-reducing bacterium from a deep subsurface Australian thermal aquifer

Bacillus subterraneus sp. nov., an iron- and manganese-reducing bacterium from a deep subsurface Australian thermal aquifer

The phylogenetic diversity of Thermus and Meiothermus from microbial mats of an Australian subsurface aquifer runoff channel

Molecular investigation of a microbial mat associated with the Great Artesian Basin

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