Isolation of substances with antiproliferative and apoptosis-inducing activities against leukemia cells from the leaves of Zanthoxylum ailanthoides Sieb. &amp; Zucc

Chou, Su Tze; Chan, Hsiu Hui; Peng, Hsin Yi; Liou, Meei Jen; Wu, Tian Shung

doi:10.1016/j.phymed.2010.08.018

Cited by 30 publications

(18 citation statements)

References 20 publications

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“…The slides were then drained, placed on a tray and flooded slowly with three changes of neutralization buffer (0.4 M TrisHCl, pH 7.5), each for 5 min. The slides were stained with ethidium bromide (10 mg/ml), covered with a cover glass, and analyzed within 1 h at 100X magnification using a fluorescent microscope (Motic BA400, Germany) with green filter [17].…”

Section: Dna Fragmentation Studymentioning

confidence: 99%

Russell’s Viper Venom Purified Toxin Drct-II Inhibits the Cell Proliferation and Induces G1 Cell Cycle Arrest in Human Leukemic Cancer Cells

AK¹

2015

Transl Med (sunnyvale)

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The present study was an effort to establish the anticancer activity of the purified protein toxin (drCT-II) from Indian Russell's viper (Daboia russelli russelli) venom in leukemic cell line and animal model. Isolation and purification of drCT-II was done through CM-cellulose ion exchange chromatography and RP-HPLC. SDS-PAGE molecular weight and first 20 amino acid sequence of drCT-II was done. The anti-leukemic activity using U937 and K562 cell line was established through cytotoxicity, apoptosis, cell cycle study, morphology, cancer marker proteins. The mean survival time of EAC induced male albino mice was established. Human lymphocyte cytotoxicity was done. drCT-II was eluted with 0.1 M NaCl on CM-cellulose ion exchange chromatography. On RP-HPLC, drCT-II produced single peak with retention time of 14.6 min. SDS-PAGE molecular weight was found to be 6.6 KDa and the first 20 amino acid sequence was found to be LQXNKLVPIASKTXPPGKNL. drCT-II produced time and dose dependent cell (U937 and K562) growth inhibition. The IC50 was found to be 35.5 µg/ml for U937 cell and 48.2 µg/ml for K562 cells. drCT-II produced membrane disruption, blebbing and nuclear disintegration in U937 and K562 cells observed through confocal and scanning electron microscopy. It exhibited DNA fragmentation and comet formation in leukemic cells. drCT-II produced apoptosis, cell cycle arrest at G 1 phase and increased the expression of P 21 , P 27 and P 53 . drCT-II induced apoptosis in leukemic cells was followed through caspase 3 and 9 pathway activation. EAC cell growth in male albino mice was significantly inhibited by drCT-II, thus increased the mean survival time. drCT-II significantly reduced the human lymphocyte count (in culture). It may be concluded that drCT-II, a 6.6 KDa protein purified from Daboia russelli russelli venom would be a novel pro-apoptotic agent that induced cancer cell killing through p53 and caspase pathway.

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Section: Dna Fragmentation Studymentioning

confidence: 99%

Russell’s Viper Venom Purified Toxin Drct-II Inhibits the Cell Proliferation and Induces G1 Cell Cycle Arrest in Human Leukemic Cancer Cells

AK¹

2015

Transl Med (sunnyvale)

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“…Finding a cure for this disease is always an important objective for human endeavor. Natural products have long been considered as potential drug candidates for cancer prevention and treatment (Chou et al, 2011). Zanthoxylum species are potential sources for find new antitumor agents, because diverse substances obtained from some of this species have showed strong citotoxic activity against different tumor cell lines.…”

Section: Citotoxic Activitymentioning

confidence: 99%

“…From this fraction were obtained four pheophorbide derivatives, where three of these compounds showed cytotoxic activities against both leukemia cells with IC 50 value in the range of 46.76-79.43 nM (Chou et al, 2011).…”

Section: Citotoxic Activitymentioning

confidence: 99%

Zanthoxylum Genus as Potential Source of Bioactive Compounds

Patiño¹,

Prieto²,

Cuca³

2012

Bioactive Compounds in Phytomedicine

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“…3c) as compared to LPS treatment alone. anti-vascular inflammatory, antiproliferative, antinociceptive and anticaries potentials (Guo et al, 2010;Li et al, 2010;Pereira et al, 2010;Chou et al, 2011;Rodriguez-Guzman et al, 2011). Unfortunately, the scientific evidence of LPFE (Z. acanthopodium DC.)…”

Section: Effects Of Lpfe On the Protein And Gene Expression Of Inflammentioning

confidence: 99%

Lemon Pepper Fruit Extract (<i>Zanthoxylum acanthopodium</i> DC.) Suppresses the Expression of Inflammatory Mediators in Lipopolysaccharide-Induced Macrophages <i>In Vitro</i>

Yanti¹,

Pramudito²,

Nuriasari³

et al. 2011

American Journal of Biochemistry and Biotechnology

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Problem statement: Lemon pepper fruits (Zanthoxylum acanthopodium DC.; Rutaceae) have been used as a traditional source against stomach ache by Batak people in North Sumatera province, Indonesia. However, its scientific evidence for treatment of inflammatory disorders particularly gastritis has not been reported. Approach: Here, we investigated the inhibitory effects of Lemon Pepper Fruit Extract (LPFE) against inflammatory biomarkers by conducting cell culture experiments in vitro. The fruits of lemon pepper were dried and extracted twice in 70% ethanol, followed by evaporation and freeze-drying. The concentrated extract was further tested for its potential inhibition on the protein and gene expression of several inflammatory biomarkers, i.e. ) and LPS (2 µg mL −1 ) had no cytotoxicity effects on macrophages. LPFE dose dependently decreased the expression of TNF-α and COX-2 proteins and MMP-9 activity in macrophages treated with LPS. At the gene level, LPFE were effectively found to block the mRNA expression of TNF-α, IL-6, iNOS, COX-2 and MMP-9. Conclusion: Our results suggest that LPFE significantly inhibits selected inflammatory biomarkers at the protein and gene levels in LPS-induced macrophages. Further in vivo study using animal models is needed to determine the exact anti-inflammatory potential of LPFE.

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Isolation of substances with antiproliferative and apoptosis-inducing activities against leukemia cells from the leaves of Zanthoxylum ailanthoides Sieb. & Zucc

Cited by 30 publications

References 20 publications

Russell’s Viper Venom Purified Toxin Drct-II Inhibits the Cell Proliferation and Induces G1 Cell Cycle Arrest in Human Leukemic Cancer Cells

Russell’s Viper Venom Purified Toxin Drct-II Inhibits the Cell Proliferation and Induces G1 Cell Cycle Arrest in Human Leukemic Cancer Cells

Zanthoxylum Genus as Potential Source of Bioactive Compounds

Lemon Pepper Fruit Extract (<i>Zanthoxylum acanthopodium</i> DC.) Suppresses the Expression of Inflammatory Mediators in Lipopolysaccharide-Induced Macrophages <i>In Vitro</i>

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