Isolation of surface (S) layer protein carrying Lactobacillus species from porcine intestine and faeces and characterization of their adhesion properties to different host tissues

Jakava-Viljanen, Miia; Palva, Airi

doi:10.1016/j.vetmic.2007.04.029

Cited by 73 publications

(44 citation statements)

References 29 publications

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“…The scarcity of available data on the bacterial populations of treated urban or animal effluents could explain the small number of sequence matches, particularly with the Eubacterium-Clostridium group. Peak C5 was closely related (98% similarity) to an uncultured Clostridium previously found in a manure storage pit (58), and peak BSL7 was identified as L. sobrius, which has previously been described in piglet (32) and pig feces (28). However, none of these peaks was found in treated manure whereas the two Bifidobacterium peaks were found in both raw and treated manure.…”

Section: Discussionsupporting

confidence: 54%

Pig Manure Contamination Marker Selection Based on the Influence of Biological Treatment on the Dominant Fecal Microbial Groups

Marti¹,

Dabert²,

Pourcher³

2009

Appl Environ Microbiol

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The objective of this study was to identify a microbial marker for pig manure contamination. We quantified the persistence of four dominant bacterial groups from the pig intestinal tract throughout manure handling at 10 livestock operations (including aerobic digestion) by using molecular typing. The partial 16S rRNA genes of Bacteroides-Prevotella, Eubacterium-Clostridiaceae, Bacillus-Streptococcus-Lactobacillus (BSL), and Bifidobacterium group isolates were amplified and analyzed by capillary electrophoresis single-strand conformation polymorphism. The most dominant bacterial populations were identified by cloning and sequencing their 16S rRNA genes. The results showed that Bifidobacterium spp. and, to a lesser extent, members of the BSL group, were less affected by the aerobic treatment than either Eubacterium-Clostridiaceae or Bacteroides-Prevotella. Two Bifidobacterium species found in raw manure were still present in manure during land application, suggesting that they can survive outside the pig intestinal tract and also survive aerobic treatment. The 16S-23S rRNA internal transcribed spacer of one species, Bifidobacterium thermacidophilum subsp. porcinum, was sequenced, and a specific pair of primers was designed for its detection in the environment. With this nested PCR assay, this potential marker was not detected in samples from 30 bovine, 30 poultry, and 28 human fecal samples or in 15 urban wastewater effluents. As it was detected in runoff waters after spreading of pig manure, we propose this marker as a suitable microbial indicator of pig manure contamination.

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Section: Discussionsupporting

confidence: 54%

Pig Manure Contamination Marker Selection Based on the Influence of Biological Treatment on the Dominant Fecal Microbial Groups

Marti¹,

Dabert²,

Pourcher³

2009

Appl Environ Microbiol

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“…Indeed, isolated subunits of S-layers from a number of bacteria have the capability to recrystallize in suspension, at liquid-surface interfaces, on lipid structures, and on solid supports. [6,[10][11][12][13][14][15] As demonstrated through a number of recent reports, S-layers from Bacillaceae species have great promise for use in nanotechnological applications. [11,[16][17][18][19][20] Bacillaceae S-layers have an N-terminal region named the S-layer homology (SLH) domain that is responsible for anchoring the protein subunits to secondary cell wall polymers (SCWPs) in the underlying rigid cell envelope layer.…”

Section: Introductionmentioning

confidence: 99%

Entropically Driven Self‐Assembly of Lysinibacillus sphaericus S‐Layer Proteins Analyzed Under Various Environmental Conditions

Teixeira¹,

Strickland²,

Mark³

et al. 2010

Macromolecular Bioscience

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S-Layer proteins are an example of bionanostructures that can be exploited in nanofabrication. In addition to their ordered structure, the ability to self-assembly is a key feature that makes them a promising technological tool. Here, in vitro self-assembly kinetics of SpbA was investigated, and found that it occurs at a rate that is dependent on temperature, its concentration, and the concentration of calcium ions and sodium chloride. The activation enthalpy (120.81 kJ . mol(-1)) and entropy (129.34 J . mol(-1) . K(-1)) obtained infers that the incorporation of monomers incurs in a net loss of hydrophobic surface. By understanding how the protein monomers drive the self-assembly at different conditions, the rational optimization of this process was feasible.

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“…amylovorus is one of the dominant S-layer-carrying Lactobacillus species in pigs, showing strong adherence to the pig intestinal epithelial cells and being of interest because of its potential probiotic properties (7,13).…”

mentioning

confidence: 99%

Genome Sequence of Lactobacillus amylovorus GRL1118, Isolated from Pig Ileum

et al. 2011

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Isolation of surface (S) layer protein carrying Lactobacillus species from porcine intestine and faeces and characterization of their adhesion properties to different host tissues

Cited by 73 publications

References 29 publications

Pig Manure Contamination Marker Selection Based on the Influence of Biological Treatment on the Dominant Fecal Microbial Groups

Pig Manure Contamination Marker Selection Based on the Influence of Biological Treatment on the Dominant Fecal Microbial Groups

Entropically Driven Self‐Assembly of Lysinibacillus sphaericus S‐Layer Proteins Analyzed Under Various Environmental Conditions

Genome Sequence of Lactobacillus amylovorus GRL1118, Isolated from Pig Ileum

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