Isolation of Temperature-Sensitive Mutants of Arabidopsis thaliana That Are Defective in the Redifferentiation of Shoots

Yasutani, Izumi; Ozawa, Shoichi; Nishida, Toshiaki; Sugiyama, Masaru; Komamine, Atsushi

doi:10.1104/pp.105.3.815

Cited by 57 publications

(48 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…More importantly, the efficiency of shoot induction obtained in the proposed IZE-based protocol is similar to that reported in seedling root culture (Feldman and Marks, 1986), which thus far has been the one most frequently employed in genetic studies on regeneration induced in vitro (Yasutani et al, 1994;Banno et al, 2001;Zhao et al, 2002;Daimon et al, 2003). In both of these systems, IZE and root-derived, a sequence of two media with opposite auxin-to-cytokinin ratios results in de-differentiation of primary explant tissue and subsequent shoot induction during 3-week culture.…”

Section: Discussionsupporting

confidence: 58%

Immature Zygotic Embryo Cultures of Arabidopsis. A Model System for Molecular Studies on Morphogenic Pathways Induced In Vitro

Kraut¹,

Wójcikowska²,

Ledwoń³

et al. 2011

Acta Biologica Cracoviensia Series Botanica

View full text Add to dashboard Cite

To understand the molecular mechanism controlling in vitro plant morphogenesis, a culture system enabling induction of alternative morphogenic pathways (somatic embryogenesis, SE; shoot organogenesis, ORG) in a well defined population of somatic cells is needed. Arabidopsis is the most useful model plant for genomic studies, but a system in which SE or ORG can be induced alternatively in the same type of explant has not been proposed. Immature zygotic embryos (IZEs) of Arabidopsis provide the only explants with embryogenic potential, and have been recommended for studying mechanisms of SE induced in vitro. This study was aimed at defining culture conditions promoting induction of alternative morphogenic pathways: shoot ORG in IZE explants. The established protocol involves pretreatment of IZE explants with liquid auxin-rich callus induction (CIM) medium, followed by subculture on solid cytokinin-rich shoot induction medium (SIM). The method enables efficient shoot induction in Columbia (Col-0) and Wassilewskija (Ws), genotypes commonly used in molecular studies. During 3 weeks of culture up to 90% of Col-0 and 70% of Ws explants regenerated shoots via an indirect morphogenic pathway. We analyzed the qRT-PCR expression patterns of the LEC (LEC1, LEC2 and FUS3) genes, the key regulators of Arabidopsis embryogenesis, in the IZE explants induced to promote shoot ORG. The sharp decline of LEC expression on SIM medium confirmed that culture of Arabidopsis IZE explants enables experimental manipulation of the morphogenic response of somatic cells. A scheme illustrating various in vitro morphogenic responses of IZEs in relation to hormonal treatment is presented.K Ke ey y w wo or rd ds s: : Arabidopsis, immature zygotic embryos, in vitro morphogenesis, shoot regeneration.

show abstract

Section: Discussionsupporting

confidence: 58%

Immature Zygotic Embryo Cultures of Arabidopsis. A Model System for Molecular Studies on Morphogenic Pathways Induced In Vitro

Kraut¹,

Wójcikowska²,

Ledwoń³

et al. 2011

Acta Biologica Cracoviensia Series Botanica

View full text Add to dashboard Cite

show abstract

“…Previously, we characterized temperature-sensitive mutants of A. thaliana to dissect the process of shoot and root regeneration via callus formation (Yasutani et al, 1994;Ozawa et al, 1998;Sugiyama, 2003). Here we extend this approach to elucidate the process of adventitious root formation.…”

Section: Introductionmentioning

confidence: 99%

“…was used as the wild type. The srd2 mutant of Ler origin, which was isolated as temperature-sensitive for shoot redifferentiation (Yasutani et al, 1994) and later also shown to be defective in dedifferentiation and root development (Ozawa et al, 1998), was used together with newly isolated mutants. The scarecrow-2 (scr-2) mutant in the Wassilewskija background (Scheres et al, 1995) and the microtubule organization 1-1 (mor1-1) mutant in the Columbia background (Whittington et al, 2001) were kindly provided by Dr Ben Scheres (Utrecht University, The Netherlands) and Dr Geoffrey O. Wasteneys (The Australian National University, Australia), respectively.…”

Section: Plant Materialsmentioning

confidence: 99%

Genetic analysis of adventitious root formation with a novel series of temperature-sensitive mutants ofArabidopsis thaliana

2003

View full text Add to dashboard Cite

When cultured on media containing the plant growth regulator auxin,hypocotyl explants of Arabidopsis thaliana generate adventitious roots. As a first step to investigate the genetic basis of adventitious organogenesis in plants, we isolated nine temperature-sensitive mutants defective in various stages in the formation of adventitious roots: five root initiation defective(rid1 to rid5) mutants failed to initiate the formation of root primordia; in one root primordium defective (rpd1)mutant, the development of root primordia was arrested; three root growth defective (rgd1, rgd2, and rgd3) mutants were defective in root growth after the establishment of the root apical meristem. The temperature sensitivity of callus formation and lateral root formation revealed further distinctions between the isolated mutants. The rid1mutant was specifically defective in the reinitiation of cell proliferation from hypocotyl explants, while the rid2 mutant was also defective in the reinitiation of cell proliferation from root explants. These two mutants also exhibited abnormalities in the formation of the root apical meristem when lateral roots were induced at the restrictive temperature. The rgd1and rgd2 mutants were deficient in root and callus growth, whereas the rgd3 mutation specifically affected root growth. The rid5 mutant required higher auxin concentrations for rooting at the restrictive temperature, implying a deficiency in auxin signaling. The rid5 phenotype was found to result from a mutation in the MOR1/GEM1 gene encoding a microtubule-associated protein. These findings about the rid5 mutant suggest a possible function of the microtubule system in auxin response.

show abstract

“…Munetaka Sugiyama and colleagues established an experimental system suitable for studying callus, root, and/or shoot induction from Arabidopsis explants (Yasutani et al 1994;Ozawa et al 1998). Using this system, they isolated and characterized many mutants, which led to the identification of genes involved with dedifferentiation and also in organogenesis.…”

Section: Study Of Organogenesis In Arabidopsismentioning

confidence: 99%

Atsushi Komamine (1929–2011): a tribute

Kodama

2014

Plant Biotechnol Rep

View full text Add to dashboard Cite

Atsushi Komamine was one of the pioneers of plant tissue culture in Japan and a researcher with many friends throughout the world. His objective was to determine the potential capacities of plant cells, and he contributed considerably to plant tissue cultivation research by establishing several unique culture systems that controlled cell division and differentiation. He emphasized that the uniformity of cultured cells and the synchrony of the plant cell response at a high frequency were necessary to study the functions of plant cells using biochemical and molecular biological methods. He trained over 300 people, and many outstanding individuals in academia and industry emerged under his tutelage. He was an outstanding mentor: with his accommodating attitude, he was loved dearly by students and foreign researchers alike. He set up the Asia Pacific Association of Plant Tissue Culture and Agribiotechnology in 2000, and he established the international journal Plant Biotechnology Reports with Jang R. Liu in 2006, thereby contributing to Asian plant sciences. In addition to describing his achievements and activities, this article aims to paint a portrait of the man himself.

show abstract

Isolation of Temperature-Sensitive Mutants of Arabidopsis thaliana That Are Defective in the Redifferentiation of Shoots

Cited by 57 publications

References 14 publications

Immature Zygotic Embryo Cultures of Arabidopsis. A Model System for Molecular Studies on Morphogenic Pathways Induced In Vitro

Immature Zygotic Embryo Cultures of Arabidopsis. A Model System for Molecular Studies on Morphogenic Pathways Induced In Vitro

Genetic analysis of adventitious root formation with a novel series of temperature-sensitive mutants ofArabidopsis thaliana

Atsushi Komamine (1929–2011): a tribute

Contact Info

Product

Resources

About