Isolation of tetrameric microsatellite markers and its application on parentage identification in Procambarus clarkii

Wang, Qishuai; Hu, Qian; Yang, Siqi; Li, Yanhe

doi:10.1007/s10499-023-01072-0

Cited by 5 publications

(3 citation statements)

References 38 publications

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“…In golden mandarin fish, S. scherzeri, 110 of 120 offspring were accurately assigned to their correct parent pairs using seven microsatellite markers [19]. When seven informative microsatellite loci were used, the correct parentage of assignments' success was more than 80.0% in Procambarus clarkii [44]. The markers for parentage determination were considered applicable when discrimination power was more than 80% [45].…”

Section: Discussionmentioning

confidence: 99%

Parentage Determination of Chinese Hooksnout Carp (Opsariichthys bidens) Based on Microsatellite DNA Markers

Ge,

Xu,

Ren

et al. 2024

Aquaculture Research

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In this study, a panel of 14 microsatellite markers was screened to determine the parentage of 144 progenies in 12 Chinese hooksnout carp (Opsariichthys bidens) full-sib families. The combined exclusion probabilities for 14 loci were 97.6% and 99.9%, respectively, when there was no available parent information or only one parent information was provided. Simulation analysis demonstrated that the power of five loci to exclude false parents exceeded 99.0%, while that of eight loci reached 99.9% based on allele frequency data obtained from full-sib families (168 individuals). Moreover, the cumulative assignment success rate reached 100% with known parental and filial information when utilizing 13 or more loci, even in cases where no parent information was available. The results showed that this set of microsatellite markers proved to be a reliable and efficient tool for parentage determination of O. bidens.

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Section: Discussionmentioning

confidence: 99%

Parentage Determination of Chinese Hooksnout Carp (Opsariichthys bidens) Based on Microsatellite DNA Markers

Ge,

Xu,

Ren

et al. 2024

Aquaculture Research

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“…Currently, studies on crayfish are mainly focused on environmental stress [ 12 ], culture diseases [ 13 ], nutritional quality [ 14 ], seed breeding [ 15 ], etc., and there are still few studies on morphology. Zhang et al analyzed the effects of morphological traits on body weight of crayfish of different sexes by using path analysis [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Different Cultivation Modes on Morphological Traits and Correlations between Traits and Body Mass of Crayfish (Procambarus clarkii)

Li,

Qin,

Tian

et al. 2024

Biology

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In this study, juvenile crayfish hatched from the same population were cultured in different growing environments: pond (D1), paddy field (D2), and aquaculture barrel (D3), and fed for 60 days. Crayfishes were selected randomly, females and males, 50 tails each from six groups (D1-♀, D1-♂, D2-♀, D2-♂, D3-♀, D3-♂) to measure the following morphological traits: full length (X1), body length (X2), chelicerae length (X3), chelicerae weight (X4), cephalothorax length (X5), cephalothorax width (X6), cephalothorax height (X7), eye spacing (X8), caudal peduncle length (X9), and caudal peduncle weight (X10). We found that the coefficient of variation (CV) of X4 was the largest in each culture mode, and males (28.58%~38.67%) were larger than females (37.76%~66.74%). The CV of X4 of crayfish cultured in D1 and D2 was larger than that of D3. All traits except X8 were positively correlated with body weight (p < 0.05). After pathway analysis, we found that X4, X5, X7, and X10 were significantly correlated with the body weight of D1-♀; the equation was YD1-♀ = −29.803 + 1.249X4 + 0.505X5 + 0.701X7 + 1.483X10 (R2 = 0.947). However, X2, X4, and X6 were significantly correlated with the body weight of D1-♂; the equation was YD1-♂ = −40.881 + 0.39X2 + 0.845X4 + 1.142X6 (R2 = 0.927). In D2-♀, X1, X4, X5, and X10 were significantly correlated with body weight; the equation was YD2-♀ = −12.248 + 0.088X1 + 1.098X4 + 0.275X5 + 0.904X10 (R2 = 0.977). X4 and X5 played a major role in the body weight of D2-♂ with the equation: YD2-♂ = −24.871 + 1.177X4 + 0.902X5 (R2 = 0.973). X3 and X10 mainly contributed to the body weight of D3-♀ with the equation: YD3-♀ = −22.476 + 0.432X3 + 3.153X10 (R2 = 0.976). X1 and X4 mainly contributed to the body weight of D3-♂ with the equation: YD3-♂ = −34.434 + 0.363X1 + 0.669X4 (R2 = 0.918). Comparing the pathway analysis with the gray relation analysis, we could conclude that the traits most correlated with body weight in D1-♀ were X10 and X7; in D1-♂, X6; in D2-♀, X10, X1, and X5; in D2-♂, X5; in D3-♀, X10; and in D3-♂, X4 and X1.

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“…They have many advantages as molecular markers: high polymorphism, genetic codominance, easy detection, and low requirements for template DNA compared with that for other genetic markers [15]. They can be used for population genetics [16][17][18][19], pedigree analysis [20][21][22], and population genetic-map construction [23][24][25]. Microsatellite markers are also important in aquaculture, contributing to the conservation and improvement of aquaculture species through the genetic analysis of the species, helping to understand species diversity, phylogeny, evolution, and development and providing a basis for the sustainable conservation and development of aquaculture [26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Microsatellite-Marker-Based Evaluation of Stock Enhancement for Kuruma Prawn Penaeus japonicus in Beibu Gulf, South China Sea

Zhang,

Zhao,

Shan

et al. 2023

Fishes

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The objectives of this study were to evaluate the effect of stock enhancement on Penaeus japonicus (Bate, 1888) in Beibu Gulf, South China Sea, and to investigate whether stock enhancement has genetically affected the natural population of P. japonicus in the Beibu Gulf waters. Five microsatellite loci were used to identify released individuals among recaptured specimens. In total, 13 of the 487 recaptured individuals were found with their corresponding parents for this release of shrimp seedlings, accounting for 2.67% of the total recaptured P. japonicus. Furthermore, it was determined that the genetic differences among the broodstock, released, and recaptured populations of P. japonicus were small and did not cause significant changes in the genetic structure of P. japonicus in the area. In conclusion, the stock enhancement of P. japonicus in Beibu Gulf had a certain effect on resource restoration, and there is no difference in the genetic characteristics obtained by the microsatellite markers compared to the original population in this area.

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Isolation of tetrameric microsatellite markers and its application on parentage identification in Procambarus clarkii

Cited by 5 publications

References 38 publications

Parentage Determination of Chinese Hooksnout Carp (Opsariichthys bidens) Based on Microsatellite DNA Markers

Parentage Determination of Chinese Hooksnout Carp (Opsariichthys bidens) Based on Microsatellite DNA Markers

Effects of Different Cultivation Modes on Morphological Traits and Correlations between Traits and Body Mass of Crayfish (Procambarus clarkii)

Microsatellite-Marker-Based Evaluation of Stock Enhancement for Kuruma Prawn Penaeus japonicus in Beibu Gulf, South China Sea

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