Isolation of the plastid FtsZ gene from Cyanophora paradoxa (Glaucocystophyceae, Glaucocystophyta)

Sato, Mayuko; Nishikawa, Toshikazu; Yamazaki, T.; Kawano, Shigeyuki

doi:10.1111/j.1440-183.2005.00376.x

Cited by 9 publications

(12 citation statements)

References 17 publications

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“…Glaucophyte chloroplast division involves FtsZbased division machinery on the stromal side (Sato et al, 2005, and a structure similar to the inner PD ring has been detected by electron microscopy. However, the outer PD ring is not evident (Iino and Hashimoto, 2003;Sato et al, 2009).…”

Section: Chloroplasts In Glaucophytes and Intermediates Between Cyanomentioning

confidence: 99%

Mechanism of Plastid Division: From a Bacterium to an Organelle

Miyagishima

2011

Plant Physiology

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Section: Chloroplasts In Glaucophytes and Intermediates Between Cyanomentioning

confidence: 99%

Mechanism of Plastid Division: From a Bacterium to an Organelle

Miyagishima

2011

Plant Physiology

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“…Two FtsZ families, FtsZA and FtsZB, are also present in red algae and likewise form heteropolymers (Miyagishima and Kabeya, 2010;TerBush et al, 2013;Chen et al, 2017). Cyanophora paradoxa, a glaucophyte alga, contains only a single plastidic FtsZ (Sato et al, 2005;Leger et al, 2015). The plastids (cyanelles) in C. paradoxa retain a peptidoglycan wall, and septal peptidoglycan synthesis is required for normal cyanelle division (Sato et al, 2007).…”

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confidence: 99%

“…Plastidic FtsZs are nuclear-encoded and targeted to the stroma, where they assemble into the midplastid Z ring (Osteryoung and Vierling, 1995;McAndrew et al, 2001;Vitha et al, 2001;El-Kafafi et al, 2005;Sato et al, 2005Sato et al, , 2009). FtsZ1 and FtsZ2 are both critical for normal chloroplast division, as mutants lacking either FtsZ bear larger and fewer chloroplasts (Osteryoung et al, 1998;Strepp et al, 1998;McAndrew et al, 2008;Schmitz et al, 2009).…”

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confidence: 99%

Conserved Dynamics of Chloroplast Cytoskeletal FtsZ Proteins Across Photosynthetic Lineages

et al. 2017

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The cytoskeletal Filamenting temperature-sensitive Z (FtsZ) ring is critical for cell division in bacteria and chloroplast division in photosynthetic eukaryotes. While bacterial FtsZ rings are composed of a single FtsZ, except in the basal glaucophytes, chloroplast division involves two heteropolymer-forming FtsZ isoforms: FtsZ1 and FtsZ2 in the green lineage and FtsZA and FtsZB in red algae. FtsZ1 and FtsZB probably arose by duplication of the more ancestral FtsZ2 and FtsZA, respectively. We expressed fluorescent fusions of FtsZ from diverse photosynthetic organisms in a heterologous system to compare their intrinsic assembly and dynamic properties. FtsZ2 and FtsZA filaments were morphologically distinct from FtsZ1 and FtsZB filaments. When coexpressed, FtsZ pairs from plants and algae colocalized, consistent with heteropolymerization. Fluorescence recovery after photobleaching experiments demonstrated that subunit exchange was greater from FtsZ1 and FtsZB filaments than from FtsZ2 and FtsZA filaments and that FtsZ1 and FtsZB increased turnover of FtsZ2 and FtsZA, respectively, from heteropolymers. GTPase activity was essential only for turnover of FtsZ2 and FtsZA filaments. Cyanobacterial and glaucophyte FtsZ properties mostly resembled those of FtsZ2 and FtsZA, though the glaucophyte protein exhibited some hybrid features. Our results demonstrate that the more ancestral FtsZ2 and FtsZA have retained functional attributes of their common FtsZ ancestor, while eukaryotic-specific FtsZ1 and FtsZB acquired new but similar dynamic properties, possibly through convergent evolution. Our findings suggest that the evolution of a second FtsZ that could copolymerize with the more ancestral form to enhance FtsZ-ring dynamics may have been essential for plastid evolution in the green and red photosynthetic lineages.

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“…This alga has been examined extensively as a model organism of primitive phototrophs (e.g. Sato et al 2005, Watanabe et al 2009, Frassanito et al 2010, Leblond et al 2010, Facchinelli et al 2013. Ultrastructural features of C. paradoxa were examined using transmission and scanning electron microscopy (TEM and SEM, respectively) to demonstrate that the cell periphery lacks a cell wall but consists of plasma membrane and flattened vesicles containing a plate just underneath the membrane (Hall and Claus 1963, Mignot et al 1969, Trench et al 1978, Kies 1979, Kugrens et al 1999.…”

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confidence: 99%

Surface Ornamentation of <i>Cyanophora paradoxa</i> (Cyanophorales, Glaucophyta) Cells as Revealed by Ultra-High Resolution Field Emission Scanning Electron Microscopy

et al. 2014

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Summary Cyanophora paradoxa is an enigmatic biflagellate that may represent the first photosynthetic eukaryote morphologically. This alga has been widely studied as a model organism of primitive phototrophs. However, surface ornamentations of the vegetative cells have not been examined using ultra-high resolution field emission (FE) scanning electron microscopy (SEM). In the present study, C. paradoxa NIES-547 vegetative cells were examined using FE-SEM and compared with the data using conventional SEM. Our FE-SEM images demonstrated that the cell surface was ornamented with angular fenestrations framed by ridges. In contrast, conventional SEM did not reveal similar surface ornamentation. Transmission electron microscopy showed the ridge was formed by the edges of overlapping or attaching outermost plate vesicles at the cell periphery.

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Isolation of the plastid FtsZ gene from Cyanophora paradoxa (Glaucocystophyceae, Glaucocystophyta)

Cited by 9 publications

References 17 publications

Mechanism of Plastid Division: From a Bacterium to an Organelle

Mechanism of Plastid Division: From a Bacterium to an Organelle

Conserved Dynamics of Chloroplast Cytoskeletal FtsZ Proteins Across Photosynthetic Lineages

Surface Ornamentation of <i>Cyanophora paradoxa</i> (Cyanophorales, Glaucophyta) Cells as Revealed by Ultra-High Resolution Field Emission Scanning Electron Microscopy

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