Isolation of the primordial germ cells from the giant freshwater prawn,<i>Macrobrachium rosenbergii</i>

Damrongphol, Praneet; Ykarnjanasin, Alongkorn Amnua; Poolsanguan, Boonserm

doi:10.1080/07924259.2000.9652403

Cited by 7 publications

(6 citation statements)

References 15 publications

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“…In decapod crustaceans, which include the giant fresh water prawn, oogenesis begins with mitotic divisions of oogonia (Og) at a centralized germinative zone of the ovary (Damrongphol et al, 2001). As secondary Og develop, they increase in size, enter the first stage of meiotic division, and migrate away from the zone of proliferation toward the periphery of the ovarian follicles (Kruevaisayawan et al, 2010; Meeratana and Sobhon, 2007; Okumura et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Ultrastructure of differentiating oocytes and vitellogenesis in the giant freshwater prawn, Macrobrachium rosenbergii (de man)

Soonklang

Wanichanon

Stewart

et al. 2012

Microscopy Res & Technique

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The ultrastructure of oogenesis in Macrobrachium rosenbergii, with reference to vitellogenesis, has not been reported. We used light and electron microscopy, as well as vitellin (Vn) purification and antibody production, to study the temporal and spatial production of Vn in the ovary by immunofluorescence. Histologically, the ovary is subdivided into cone-shaped ovarian pouches with a central core containing layers of oogonia. These divide to produce oocytes that migrate outwardly and differentiate into mature oocytes. During the course of differentiation, oocytes undergo modifications, including the rearrangement of nuclear chromatin, the accumulation of ribosomes, rough endoplasmic reticulum (RER), and lipid, and the formation of secretory and yolk granules, resulting in four stages. Ultrastructurally, early previtellogenic oocytes (Oc(1)) are characterized by the accumulation of new ribosomal aggregates, translocated from the nucleus. Late previtellogenic oocytes (Oc(2)) show nuclear heterochromatin with a "clock face" pattern, the presence of RER, and three types of secretory granules. Follicular cells occupy the intercellular spaces and surround the Oc(2). Early vitellogenic oocytes (Oc(3)) are larger, with nuclei containing predominantly decondensed euchromatin, and cytoplasm with yolk and secretory granules, and few lipid droplets. Late vitellogenic oocytes (Oc(4)) are characterized by completely euchromatic nuclei, an indistinct plasma membrane, yolk platelets and secretory granules, and abundant lipid. Vitellogenin (Vg) in ovaries of M. rosenbergii consist of two main bands at MW 90 and 102 kDa. Our data indicates that Vn is present, and probably synthesized in Oc(3) and Oc(4), but there may be some undetected exogenous Vg production.

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Section: Introductionmentioning

confidence: 99%

Ultrastructure of differentiating oocytes and vitellogenesis in the giant freshwater prawn, Macrobrachium rosenbergii (de man)

Soonklang

Wanichanon

Stewart

et al. 2012

Microscopy Res & Technique

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“…2). Upon microscopic observation, the PGCs of early stage embryos, 5.5 day old, could easily be distinguished (Damrongphol et al, 2000). The cells were remarkably large and spherical in shape, each containing a large nucleus with conspicuous nucleoli (Fig.…”

Section: Resultsmentioning

confidence: 96%

“…Hatching rates were determined by scoring the number of hatched larvae over the total number of surviving embryos and larvae at 18.5 days after spawning. The number of PGCs was determined from 5.5-day-old embryos as described by DAMRONGPHOL et al (2000). Ten embryos from each treatment were randomly collected.…”

Section: Methodsmentioning

confidence: 99%

Effects of All-trans retinoic acid on germ cell development of embryos and larvae of the giant freshwater prawn, Macrobrachium rosenbergii

Pakdeenarong

Damrongphol

2006

Biologia

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Embryos of the giant freshwater prawn, Macrobrachium rosenbergii, were treated with 1, 10 or 50 µg ml −1 all-trans retinoic acid (AtRA) for 2 days. Survival and hatching rates were not affected. However, an increase in the number of primordial germ cells (PGCs), progenitors of gametes, and a slightly more advanced stage of the gonads were found in those treated with 10 or 50 µg ml −1 AtRA. Newly hatched larvae were treated with 0.1, 0.5 or 1 µg ml −1 AtRA for 2 days. Survival rates were lower in those treated with 0.5 or 1 µg ml −1 AtRA; nevertheless, the gonads were slightly more developed. The results indicated that AtRA, an active metabolite of vitamin A, affected germ cell and gonad development of embryos and the larvae of giant freshwater prawn.

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“…Detection of the PGCs in 5.5-day-old embryos was relatively simple (Damrongphol et al, 2000). The cells exhibited the typical characteristics of the PGCs, i.e., large with a prominent nucleus and conspicuous nucleoli; and the number was within an accurately countable range.…”

Section: Discussionmentioning

confidence: 99%

“…Hatching rates were determined 18.5 days after spawning by scoring the number of hatched larvae over the total number of live embryos and larvae. Moreover, the primordial germ cells (PGCs), progenitors of gametes, were identified and the number of PGCs was determined from 5.5-day-old embryos as described by Damrongphol et al (2000). Ten embryos from each dish were sampled; each embryo was placed on a clean glass slide and the external egg envelopes were teased open by a pair of fine needles before covering with a cover slip.…”

Section: Methodsmentioning

confidence: 99%

Effects of estradiol-17β on embryos and larvae of the giant freshwater prawn, Macrobrachium rosenbergii (Decapoda, Palaemonidae)

Pakdeenarong¹,

Damrongphol²

2006

Crustac

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The effects of estradiol-17β(E2) on embryos and larvae of the giant freshwater prawn, Macrobrachium rosenbergii (De Man, 1879), were investigated. Embryos, obtained from brooding females within one day of spawning, were exposed to 1, 10, or 50 µg/ml estradiol-17β for 2 days. No significant differences in growth or in survival rate with those of controls were found. However, those exposed to 10 µg/ml E2 showed an increase in hatching rate, in the number of primordial germ cells (PGCs), and in the rate of incorporation of the PGCs into the developing gonads. Similarly, newly hatched larvae exposed to 1, 5, or 10 µg/ml E2 for 2 days did not show significant differences in growth, survival, or developmental rate from those of the controls. However, larvae exposed to 5 or 10 µg/ml E2 showed slightly more developed gonads. RÉSUMÉLes effets de l'oestradiol-17β(E2) sur les embryons et les larves de la crevette géante d'eau douce, Macrobrachium rosenbergii (De Man, 1879), ont été recherchés. Les embryons, obtenus à partir des femelles ovigères, au premier jour de ponte, ont été exposés à 1, 10, ou 50 µg/ml d'oestradiol-17β pendant deux jours. Aucune différence significative de la croissance ou du taux de survie, n'a été observée par comparaison aux embryons témoins. Cependant, ceux exposés à 10 µg/ml E2 ont montré une augmentation du taux d'éclosion, du nombre de cellules germinales primaires (PGCs), et du taux d'incorporation des PGCs dans les gonades en développement. De même, les larves nouvellement écloses exposées à 1, 5, ou 10 µg/ml E2 pendant deux jours n'ont pas montré de différences significatives dans la croissance, la survie ou le taux de développement par rapport aux témoins. Cependant, les larves exposées à 5 ou 10 µg/ml E2 présentaient des gonades légèrement plus développées.

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Isolation of the primordial germ cells from the giant freshwater prawn,Macrobrachium rosenbergii

Cited by 7 publications

References 15 publications

Ultrastructure of differentiating oocytes and vitellogenesis in the giant freshwater prawn, Macrobrachium rosenbergii (de man)

Ultrastructure of differentiating oocytes and vitellogenesis in the giant freshwater prawn, Macrobrachium rosenbergii (de man)

Effects of All-trans retinoic acid on germ cell development of embryos and larvae of the giant freshwater prawn, Macrobrachium rosenbergii

Effects of estradiol-17β on embryos and larvae of the giant freshwater prawn, Macrobrachium rosenbergii (Decapoda, Palaemonidae)

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