Isolation of Thermus strains from hot composts (60 to 80 degrees C)

Beffa, Trello; Blanc, Michel; Lyon, Pierre-François; Vogt, G; Marchiani, Marcello; Fischer, Johanna Lott; Aragno, Michel

doi:10.1128/aem.62.5.1723-1727.1996

Cited by 203 publications

(72 citation statements)

References 22 publications

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“…This means that only 10% of the microbial population were detected by the cultivation on solid medium. Similar values (2AE6 · 10 8 CFU g )1 of dry matter by plate and 6AE4 · 10 10 cells g )1 of dry matter by fluorescent direct count) were detected in hot synthetic compost (Dees and Ghiorse 2001) and in the thermophilic compost of sewage sludge (Beffa et al 1996a).…”

Section: Enumeration Of Bacterial Cellssupporting

confidence: 68%

Microbiological monitoring in the biodegradation of sewage sludge and food waste

et al. 2004

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Aim: To study the microbiology of intensive, in-vessel biodegradation of a mixture of sewage sludge and vegetable food waste. Methods and Results:The biodegradation was performed in a closed reactor with the addition of a starter culture of Bacillus thermoamylovorans SW25 under conditions of controlled aeration, stirring, pH and temperature (60°C). The content of viable bacterial cells, determined by flow cytometry, increased from 5 · 10 8 g )1 of dry matter to 61 · 10 8 g )1 for 2 6 days of the process and then dropped to the initial value at the end of the process. The reductions of organic matter, 16S rRNA of methanogens and coenzyme F 420 fluorescence during 10 days of the treatment were 67, 54 and 87% of the initial values, respectively. The biodegradability of the organic matter decreased during the 10 days of the treatment from 3AE8 to 1AE3 mg CO 2 g)1 of organic matter per day. The treatment of sewage sludge and food waste at 60°C did not remove enterobacteria, which are the agents of intestinal infections, from the material. The percentage of viable enterobacterial cells, determined by fluorescent in situ hybridization (FISH) with Enterobacteriaceae-specific oligonucleotide probe and flow cytometry, varied from 1 to 14% of the viable bacterial cells. Conclusions:The mixture of sewage sludge and food waste can be degraded by the aerobic thermophilic bacteria; the starter culture of Bacillus thermoamylovorans SW25 can be used to perform this process; and enterobacteria can survive under treatment of sewage sludge and food waste at 60°C for 13 days. Significance and Impact of the Study: The results show that FISH with an oligonucleotide probe can be used to study not only the growth but also the degradation of biomass. Obtained results could be used to design the bioconversion of sewage sludge and food waste into organic fertilizer.

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Section: Enumeration Of Bacterial Cellssupporting

confidence: 68%

Microbiological monitoring in the biodegradation of sewage sludge and food waste

et al. 2004

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“…Although general tendencies with regard to thermophilic and mesophilic populations of microorganisms have been identified during composting of these ‘clean’ biowastes, knowledge of the importance of specific taxonomic groups and functional groups can still be improved (Beffa et al . 1996a; Beffa et al . 1996b; Hellmann et al .…”

Section: Introductionmentioning

confidence: 99%

Microbiological aspects of biowaste during composting in a monitored compost bin

et al. 2003

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Aims: To determine the microbial succession of the dominating taxa and functional groups of microorganisms and the total microbial activity during the composting of biowaste in a monitored process. Methods and Results: Biowaste (vegetable, fruit and garden waste) was composted in a monitored composting bin system. During the process, taxonomic and functional subpopulations of microorganisms were enumerated, and dominating colonies were isolated and identified. All counts decreased during the thermophilic phase of the composting, but increased again when the temperature declined. Total microbial activity, measured with an enzyme activity assay, decreased during the thermophilic phase, increased substantially thereafter, and decreased again during maturation. Bacteria dominated during the thermophilic phase while fungi, streptomycetes and yeasts were below the detection limit. Different bacterial populations were found in the thermophilic and mesophilic phases. In fresh wastes and during the peak-heating phase, all bacterial isolates were bacilli. During the cooling and maturation phase the bacterial diversity increased, including also other Gram-positive and Gram-negative bacteria. Among the fungi, Aspergillus spp. and Mucor spp. were predominant after the thermophilic phase. Conclusions: The microbial abundance, composition and activity changed substantially during composting and compost maturity was correlated with high microbial diversity and low activity. Significance and Impact of the Study: A more complete overview of the whole composting process of biowaste, based on microbial counts, species diversity and functional groups and abiotic parameters is presented, and the potential of a simple enzyme assay to measure total microbial activity was demonstrated.

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“…PCR reaction conditions were made according to Beffa et al . and PCR product was cloned into pGEM‐T vector system and then 16S rRNA gene sequence was determined with an Applied Biosystems model 373A DNA sequencer by using the ABI PRISM cycle sequencing kit (Macrogen, Korea). The results of 16S rRNA gene sequencing were analyzed using the EzTaxon server (http://www.eztaxon.org/) .…”

Section: Methodsmentioning

confidence: 99%

Anoxybacillus kaynarcensis sp. nov., a moderately thermophilic, xylanase producing bacterium

2012

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A novel moderately thermophilic, Gram‐positive, endospore‐forming, rod‐shaped, motile bacteria and alkaline active xylanase producing strain D1021T, was isolated from Kaynarca hot spring in the province of Izmir, Turkey and was characterized in order to determine its phylogenetic position. Growth was observed at 35–70 °C (optimum 60 °C), at pH 6.0–10.0 (optimum pH 7.0). The strain D1021T grew on a wide range of carbon sources including ribose, xylose, glucose, maltose, sucrose, arabinose, and melibiose. The major isoprenoid quinone was MK‐7. 16S rRNA gene sequence analysis showed that strain D1021T is related to members of genus Anoxybacillus, with similarities to Anoxybacillus spp. varying from 94.7 to 98.7. The major fatty acids of strain D1021T were iso‐C15:0 (57.46%) and iso‐C17:0 (13.98%). The DNA G + C content was 42.9 mol %. DNA–DNA relatedness values between Anoxybacillus spp. and D1021T were lower than 70%. On the basis of phenotypic characteristics, rpoB analysis, phylogenetic and DNA–DNA hybridization data, it was proposed that the strain D1021T (= DSM 21706T = LMG 25303T) should be placed in the genus Anoxybacillus as the type strain of a novel species, Anoxybacillus kaynarcensis sp. nov. The GenBank accession number for the 16S rRNA sequence is EU926955.

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Isolation of Thermus strains from hot composts (60 to 80 degrees C)

Cited by 203 publications

References 22 publications

Microbiological monitoring in the biodegradation of sewage sludge and food waste

Microbiological monitoring in the biodegradation of sewage sludge and food waste

Microbiological aspects of biowaste during composting in a monitored compost bin

Anoxybacillus kaynarcensis sp. nov., a moderately thermophilic, xylanase producing bacterium

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