Isolation of Tumor Cells Based on Their Distance from Blood Vessels

Kumar, Saran; Sharife, Husni; Kreisel, Tirzah; Bar-Lev, Libat; Grunewald, Myriam

doi:10.21769/bioprotoc.3628

Cited by 5 publications

(3 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 62,63 ] Under the influence of O 2 diffusion distance, hypoxic and necrotic areas will be formed at a distance of more than 100 µm from the blood vessels inside the tumor. [ 64 ] Tumor cell hypoxia mainly includes three mechanisms: chronic or diffusion‐limited hypoxia, acute or perfusion‐limited hypoxia, and anemia‐related hypoxia. [ 65 ] Hypoxia can promote the heterogeneity of tumor cells and their strong adaptability to the complex TME and even promote tumor cell development, invasion, and metastasis.…”

Section: Tme and Tme‐related Sdtmentioning

confidence: 99%

Tumor Microenvironment‐Related Sonodynamic Therapy Mediates Tumor Therapy

Wang,

Tian

et al. 2023

Advanced Therapeutics

View full text Add to dashboard Cite

Sonodynamic therapy (SDT) is a rapidly developing, safe, and noninvasive method for tumor treatment. SDT relies on ultrasound to stimulate the activity of sonosensitizers, induce the production of many reactive oxygen species (ROS), and mediate tumor cell death. The complex tumor microenvironment (TME) is the basis of tumorigenesis and tumor development. The characteristics of the TME, which are different from those of normal tissue, are essential factors in regulating the response to antitumor therapy. The therapeutic effect of SDT is affected by the TME. Therefore, the study of multifunctional sonosensitizers related to the TME is a future direction to enhance the efficacy of SDT in treating tumors. This review summarizes the characteristics of the TME, the factors that regulate it, and its role in regulating the response to sonosensitizers. It introduces strategies for enhancing the therapeutic effect of SDT by exploiting the TME and highlights the prospects of combination therapy for tumors based on the characteristics of the TME. With this paper, we hope to provide a research direction for improving the therapeutic effect of SDT and promoting its clinical translation in the future.This article is protected by copyright. All rights reserved

show abstract

Section: Tme and Tme‐related Sdtmentioning

confidence: 99%

Tumor Microenvironment‐Related Sonodynamic Therapy Mediates Tumor Therapy

Wang,

Tian

et al. 2023

Advanced Therapeutics

View full text Add to dashboard Cite

show abstract

“…Alter the phrase. Although it is generally correlated with increased necrosis, the requirement for angiogenesis is more correlated with the oxygen gradient within the tumor, with tumor inner cells exposed to lower levels of oxygen resulting in necrosis [ 54 ]. However, newly generated vasculature in tumours is variable in size, shape, architecture, and arrangement compared to normal vessels [ 55 ].…”

Section: Introductionmentioning

confidence: 99%

Research progress of exosomes in the angiogenesis of digestive system tumour

Liu,

Wu,

Sang

et al. 2024

Discov Onc

View full text Add to dashboard Cite

Malignant tumours of the digestive system cover a wide range of diseases that affect the health of people to a large extent. Angiogenesis is indispensable in the development, and metastasis of tumours, mainly in two ways: occupation or formation. Vessels can provide nutrients, oxygen, and growth factors for tumours to encourage growth and metastasis, so cancer progression depends on simultaneous angiogenesis. Recently, exosomes have been proven to participate in the angiogenesis of tumours. They influence angiogenesis by binding to tyrosine kinase receptors (VEGFR)-1, VEGFR-2, and VEGFR-3 with different affinities, regulating Yap-VEGF pathway, Akt pathway or other signaling pathway. Additionally, exosomes are potential therapeutic vectors that can deliver many types of cargoes to different cells. In this review, we summarize the roles of exosomes in the angiogenesis of digestive system tumours and highlight the clinical application prospects, directly used as targers or delivery vehicles, in antiangiogenic therapy.

show abstract

“…Because of rapid cell proliferation, oxygen demand exceeds oxygen supply. , Moreover, the increase of tumor volume leads to the difficulty of tumor cells reaching peripheral blood vasculature. This hampers oxygen and nutrient diffusion and forms a hypoxic environment in the internal part of the solid tumor tissue. − Different cellular responses may be induced depending on the exposure time to a low oxygen level . From the literature, acute hypoxia was defined as a transient hypoxic environment of less than 72 h. , Tumor cells in an acute hypoxic environment activate metabolic adaptations to survive under adverse conditions. , Reactive oxygen species (ROS) are induced to contribute to the survival and development of a solid tumor.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Internal Hypoxic Environment in Solid Tumor Tissue Using a Folding Paper System

Huang

Chong

Lei

2021

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Hypoxia is a nonphysiological oxygen tension which is common in most malignant tumors. Hypoxia stimulates complicated cell signaling networks in cancer cells, e.g., the HIF, PI3K, MAPK, and NFκB pathways. Then, cells release a number of cytokines such as VEGFA to promote the growth of peripheral blood vessels and lead to metastasis. In the current work, understanding of the internal hypoxic environment in solid tumor tissue was attempted by developing a folding paper system. A paper-based solid tumor was constructed by folding a filter paper cultured with cancer cells. The cellular response in each layer could be analyzed by disassembling the folded paper after the culture course. The result showed that an internal hypoxic environment was successfully reproduced in the paper-based solid tumor. The cells in the inner layer expressed high levels of HIF1-α and VEGFA. Hence, proliferation and migration of endothelial cells were shown to be induced by the cells located in the internal hypoxic environment. Moreover, the paper-based solid tumor was transplanted into nude mice for the study of hypoxic response and angiogenesis. The crosstalk between internal and external parts of solid tumor tissue could be analyzed by sectioning each layer of the paper-based solid tumor. This approach provides a favorable analytical method for the discovery of the interaction between cancer cells, hypoxia, and peripheral angiogenesis.

show abstract

Isolation of Tumor Cells Based on Their Distance from Blood Vessels

Cited by 5 publications

References 14 publications

Tumor Microenvironment‐Related Sonodynamic Therapy Mediates Tumor Therapy

Tumor Microenvironment‐Related Sonodynamic Therapy Mediates Tumor Therapy

Research progress of exosomes in the angiogenesis of digestive system tumour

Analysis of the Internal Hypoxic Environment in Solid Tumor Tissue Using a Folding Paper System

Contact Info

Product

Resources

About