Isolation of two cDNAs encoding MHC-DQA1 and -DQA2 from the water buffalo, Bubalus bubalis

Niranjan, S. K.; Deb, S. M.; Sharma, Arjava; Mitra, Abhijit; Kumar, Subodh

doi:10.1016/j.vetimm.2009.02.006

Cited by 17 publications

(16 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the sequence identity between the Bogr-DQA1 and Bogr-DQA2 was found to be only 85 %, similar to that of cattle (83.5 %). These results were highly similar to the finding of Niranjan et al (2009) for water buffalo. However, they demonstrated that Bubu-DQA genes have less identity (93.9 and 97.7 %) with that of cattle as compared to the sequence identity between DQA genes (85.7 %).…”

Section: Resultssupporting

confidence: 91%

“…Furthermore, other amino acid differences were observed, including 4 in the signal peptide (SP), 13 in the α2 domain, 1 in the connecting peptide (CP), 2 in the transmembrane (TM) region, and 2 in the cytoplasmic (CY) domain. Obviously, yak has more amino acid replacements than buffaloes with 45 amino acid polymorphisms (Niranjan et al, 2009). …”

Section: Resultsmentioning

confidence: 99%

“…Importantly, the remaining 12 PBS sites have the varied amino acids in both of the polypeptide chains, in- dicating that it could have association with adaptation of yak to the harsh environment. However, Indian buffaloes have an additional three specific substitutions locating at the positions 36, 94 (hydrophobic > hydrophilic), and 57 (hydrophilic > hydrophobic), leading to the opposite water affinity (Niranjan et al, 2009), which could be due to the breed characteristics because buffalo can adapt well to the tropical areas (Perera, 2011). Furthermore, the mutations, especially within the α1 domain, have an impact on the antigen binding groove and could present differential binding ability to wide profiles of antigens in different environments during the long evolutionary history for domesticated animals (Germain, 1995;Williams et al, 2002).…”

Section: Resultsmentioning

confidence: 99%

“…The three primers, which have been used successfully to clone the buffalo DQA genes (Niranjan et al, 2009), were used to amplify the complete cDNA of the yak DQA1 and DQA2 sequences. The forward primer (A1A2F: 5 -ACCTTGAGAAGAGGATGGTCCTG-3 ) was matched onto the consensus region.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Cloning and characterization of MHC-DQA1 and MHC-DQA2 molecules from yak (<i>Bos grunniens</i>)

Memon

et al. 2016

Arch. Anim. Breed.

View full text Add to dashboard Cite

Abstract. The major histocompatibility complex (MHC) plays a crucial role in the processing and presentation of antigens and in discrimination between self and non-self. The aim of this investigation was to scrutinize the structural diversity and possible duplication of the MHC-DQA genes in yak (Bos grunniens). Two cDNA sequences were amplified and designated as Bogr-DQA1 (DQA * 0101) and Bogr-DQA2 (DQA * 2001) with GenBank accession numbers JQ864314 and JQ864315, respectively. The nucleotide and amino acid sequence alignment between Bogr-DQA1 and Bogr-DQA2 molecules showed that these two identified MHC-DQA gene sequences had more similarity to alleles of specific DQA1 and DQA2 genes from other Ruminantia species than to each other. The result from phylogenic investigation also revealed that there was a larger genetic distance between these two genes than between homologous genes from different species. The presence of different bovine DQA putative motifs and the large genetic distance between Bogr-DQA1 and Bogr-DQA2 suggest that these sequences are non-allelic. Further, these results indicate that DQA gene duplication occurs in ruminants. This study will be helpful in knowing MHC diversity in common ruminants and will deepen our understanding of the variation of immunological functions, evolutionary constraints, and selective forces that affect MHC variation within and between species.

show abstract

Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Cloning and characterization of MHC-DQA1 and MHC-DQA2 molecules from yak (<i>Bos grunniens</i>)

Memon

et al. 2016

Arch. Anim. Breed.

View full text Add to dashboard Cite

show abstract

“…1). Compared to other MHC class II gene families such as the DRB, DQA and DQB, which are highly variable at the PBS (Kamath et al, 2011;Niranjan et al, 2009;Sena et al, 2011 andSun et al, 2014). The DRA gene appears to be highly conserved for residues forming the PBS region in different mammals.…”

Section: Resultsmentioning

confidence: 97%

Molecular characterization of MHC class II DRA cDNA in Deoni cattle

Swathi

Prakash

Sakaram

et al. 2018

IJAR

View full text Add to dashboard Cite

The present study was undertaken to clone and characterize DRA gene in Deoni cattle. The cDNA for the DRA gene was amplified by using specific primers designed based on available cattle sequences and purified products were cloned in competent E.coli (DH5α) strain. The full length 1013bp product of cDNA of DRA contained a single ORF of 762 nucleotides that coded for 253 amino acids translated product. Twenty four amino acids formed signal peptide while 229 constituted mature peptide. The deduced amino acid sequences resembled those of class II molecules of other species for all the conserved residues having critical functional role. But a single N-linked glycosylation site in á1 was observed in cattle and buffalo when compared to human and swine which contain a second site in α2 domain. The signal peptide was found more variable among the species compared. Comparison of nucleotide and amino acid sequences among related species and dendrogram constructed revealed that the cattle sequences are more similar to buffalo sequences.

show abstract

High genetic diversity and distribution of Bubu-DQA alleles in swamp buffaloes (Bubalus bubalis carabanesis): identification of new Bubu-DQA loci and haplotypes

et al. 2016

View full text Add to dashboard Cite

In this study, genetic diversity analysis of MHC class II-DQA locus helped in identification of 25 new Bubu-DQA nucleotide sequences in swamp buffaloes (Bubalus bubalis carabanesis, Bubu). Phylogenetic analysis revealed the distribution of the buffalo DQA sequences in two major clusters of DQA1 and DQA2 genes, sharing common lineages with corresponding cattle alleles, possibly due to trans-species evolution. However, a highly divergent sequence, Bubu-DQA*2501, homologous to cattle (BoLA) DQA3 allele, was identified, indicating the existence of an additional locus; putative DQA3 in buffalo. PCR-RFLP analysis revealed extensive duplication of DQA locus in swamp buffaloes, sharing DQA1, DQA2, and DQA3 alleles in different combinations in duplicated haplotypes. Higher dN than dS values and Wu-Kabat variability at peptide-binding regions in Bubu-DQA indicated high polymorphism with balancing selection. Levels of genetic diversity within DQA sequences and duplication in a small population of swamp buffalo indicate the genetic richness of the species, important for fitness.

show abstract

Isolation of two cDNAs encoding MHC-DQA1 and -DQA2 from the water buffalo, Bubalus bubalis

Cited by 17 publications

References 8 publications

Cloning and characterization of MHC-DQA1 and MHC-DQA2 molecules from yak (<i>Bos grunniens</i>)

Cloning and characterization of MHC-DQA1 and MHC-DQA2 molecules from yak (<i>Bos grunniens</i>)

Molecular characterization of MHC class II DRA cDNA in Deoni cattle

High genetic diversity and distribution of Bubu-DQA alleles in swamp buffaloes (Bubalus bubalis carabanesis): identification of new Bubu-DQA loci and haplotypes

Contact Info

Product

Resources

About

Isolation of two cDNAs encoding MHC-DQA1 and -DQA2 from the water buffalo, Bubalus bubalis

Cited by 17 publications

References 8 publications

Cloning and characterization of MHC-DQA1 and MHC-DQA2 molecules from yak (&lt;i&gt;Bos grunniens&lt;/i&gt;)

Cloning and characterization of MHC-DQA1 and MHC-DQA2 molecules from yak (&lt;i&gt;Bos grunniens&lt;/i&gt;)

Molecular characterization of MHC class II DRA cDNA in Deoni cattle

High genetic diversity and distribution of Bubu-DQA alleles in swamp buffaloes (Bubalus bubalis carabanesis): identification of new Bubu-DQA loci and haplotypes

Contact Info

Product

Resources

About

Cloning and characterization of MHC-DQA1 and MHC-DQA2 molecules from yak (<i>Bos grunniens</i>)

Cloning and characterization of MHC-DQA1 and MHC-DQA2 molecules from yak (<i>Bos grunniens</i>)