Isolation of Two Cytotoxic Diterpenes from the Fern Pteris multifida

Woerdenbag, Herman J.; Lutke, L Renée; Bos, Rein; Stevens, Jan F.; Hulst, Ron; Kruizinga, W.; Zu, Yinxue; Elema, E. T.; Hendriks, H.; Uden, W van; Pras, Niesko

doi:10.1515/znc-1996-9-1006

Cited by 8 publications

(5 citation statements)

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“…The small amount of DM SO present in the wells (maximal 0.5%) was proved not to affect the experiments. The cytotoxicity assay was car ried out as recently described (Kolodziej et al, 1995;Woerdenbag et al, 1996). Briefly, tumour cells were incubated with a range of concentra tions of the test compounds in microtiter plates (Greiner, Alphen a/d Rijn, The Netherlands) at 37 °C in a humidified incubator with 5% C 0 2 for a culture period of 4 days.…”

Section: Tt Assaymentioning

confidence: 99%

“…After adding a solution of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Sigma, St. Louis, MO, USA), the amount of formazan formed was mea sured spectrophotometrically at 515 nm, using an E LX 800 universal plate reader (Bio-Tek Instru ments, Winooski, VT, USA). The IC50 value (the drug concentration causing 50% growth inhibition of the tumour cells, calculated using the curve fit ting programme 'Graphpad') was used as a param eter for cytotoxicity (Woerdenbag et al, 1996).…”

Section: Tt Assaymentioning

confidence: 99%

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Structure -Cytotoxicity Relationships of a Series of Natural and Semi-Synthetic Simple Coumarins as Assessed in Two Human Tumour Cell Lines

Kolodziej

Kayser

Woerdenbag

et al. 1997

Zeitschrift Für Naturforschung C

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The cytotoxicity of 22 natural and semi-synthetic simple coumarins was evaluated in G LC4, a human small cell lung carcinoma cell line, and in CO LO 320. a human colorectal cancer cell line, using the microculture tetrazolium (MTT) assay. With IC 50 values > 100 j.i m , following a continuous (96h) incubation, most coumarins exhibited only low cytotoxicity. Several com pounds, however, displayed significant potencies. As far as the structure -cytotoxicity rela tionship is concerned, it is conspicuous that all the potentially active natural compounds possess at least two phenolic groups in either the 6,7-or 6,8-positions. In addition, the 5formyl-6-hydroxy substituted semi-synthetic analogue was found to be potent, reflecting the importance of at least two polar functions for high cytotoxicity.

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Section: Tt Assaymentioning

confidence: 99%

Section: Tt Assaymentioning

confidence: 99%

Structure -Cytotoxicity Relationships of a Series of Natural and Semi-Synthetic Simple Coumarins as Assessed in Two Human Tumour Cell Lines

Kolodziej

Kayser

Woerdenbag

et al. 1997

Zeitschrift Für Naturforschung C

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“…A partir das frondes de P. deflexa Link., Sayago et al (2001) obtiveram uma proteína inibidora de invertase e, a partir de P. multifida Poir., foram obtidos compostos com atividades antitumorais (Woerdenbag et al 1996;Lu et al 1999). Li et al (1999) isolaram compostos com propriedades antitumorais a partir de P. semipinnata L. Não foram encontrados relatos na literatura tratando de estudos químicos ou biológicos com P. denticulata, que foi objeto de avaliação quanto à atividade alelopática, no presente estudo.…”

Section: Introductionunclassified

Potencial alelopático de espécies de Pteridaceae (Pteridophyta)

et al. 2004

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RESUMO -(Potencial alelopático de espécies de Pteridaceae (Pteridophyta)). As pteridófitas exibem forte mecanismo de dominância nas áreas onde crescem, formando associações quase que puras, onde apenas poucas espécies coexistem. Extratos etanólicos de cinco espécies de Pteridaceae, nas concentrações de 250, 500 e 1.000mg.L -1 , foram avaliados por meio de bioensaios de germinação e crescimento em condições de laboratório, para verificar o potencial de atividade alelopática sobre Lactuca sativa (L.) cv. Grand rapids (alface) e Allium cepa (L.) cv. Baia periforme (cebola). Adiantopsis radiata (L.) Feé, Adiantum serratodentatum Willd. e Pteris denticulata Sw. var. denticulata foram coletadas na Fazenda Azulão, situada no município de Dourados, MS, Brasil e as espécies Adiantum tetraphyllum Willd. e Pityrogramma calomelanos (L.) Link var. calomelanos foram coletadas na Fazenda Curupi, situada em Ponta Porã, MS, Brasil. Os bioensaios realizados revelaram que: 1) os cinco extratos vegetais não interferem significativamente na germinação de alface e cebola; 2) os extratos de Adiantum serratodentatum, Adiantum tetraphyllum, Adiantopsis radiata e Pityrogramma calomelanos inibem o crescimento da radícula das plântulas de alface; 3) Adiantum serratodentatum, Adiantum tetraphyllum e Pteris denticulata inibem o crescimento do hipocótilo de alface; 4) Adiantopsis radiata, Adiantum serratodentatum e Pteris denticulata inibem tanto o crescimento da radícula quanto do coleóptilo das plântulas de cebola. Os resultados obtidos até o momento permitem inferir que os extratos etanólicos das espécies em estudo contêm substâncias que modificam o crescimento das plântulas de alface e cebola.Palavras-chave: aleloquímicos, germinação, crescimento, pteridófitas, alface, cebola ABSTRACT -(Allelopathic potential of species of Pteridaceae (Pteridophyta)). Ferns exhibit a strong pattern of dominance in the areas in which they grow, forming almost pure associations, where only a few species coexist. Ethanol extractions of five species of Pteridaceae, in concentrations of 250, 500 and 1,000mg.L -1 , were evaluated using germination and growth bioassays under laboratory conditions, to verify the potential for allelopathic activity against Lactuca sativa (L.) strain Grand rapids (lettuce) and Allium cepa (L.) strain Baia periforme (onion). Adiantopsis radiata (L.) Feé, Adiantum serratodentatum Willd. and Pteris denticulata Sw. var. denticulata were collected at the Fazenda Azulão, situated in the municipality of Dourados, MS, Brazil, while the species Adiantum tetraphyllum Willd. and Pityrogramma calomelanos (L.) Link var. calomelanos were collected at the Fazenda Curupi, situated in Ponta Porã, MS, Brazil. The bioassays carried out revealed the following: 1) the five vegetal extracts did not significantly interfere with the germination of the lettuce or onion; 2) the extracts of Adiantum serratodentatum, Adiantum tetraphyllum, Adiantopsis radiata, and Pityrogramma calomelanos inhibited the radicular growth of the lettuce seedlings; 3) Adiantum se...

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“…3e) were shown to have cytotoxic and antimutagenic effects due to the presence of bioflavonoids (Lee and Lin, 1988;Silva et al, 1995;Sun et al, 1997;Lee et al, 1999;Lin et al, 2000;Su et al, 2000;Chen JJ et al, 2005;Gayathri et al, 2005;Woo et al, 2005;Gao et al, 2007;Shi et al, 2008;Tan et al, 2009), while Pteris semipinnata (Fig. 4c) and P. multifida have cytotoxic effects due to the presence of diterpenes (Woerdenbag et al, 1996;Li et al, 1998Li et al, , 1999. Several authors have studied the anti-cancer potentials of ferns and lycopods, namely P. aquilinum, Davallia cylindrical, Stenoloma chusanum, Selaginella frondosa, Thelypteris torresiana, P. vittata, Selaginella ciliaris, Marsilea minuta, and Thelypteris prolifera (Chiu et al, 2009;Sarker et al, 2011;Tomsik, 2013;Zhang et al, 2013;Kaur et al, 2014;Xia et al, 2014).…”

Section: Anti-cancer Antidiabetic and Antiviral Activitymentioning

confidence: 99%

A review of the use of pteridophytes for treating human ailments

Xavier-ravi

Vigila

Zhang

et al. 2018

J. Zhejiang Univ. Sci. B

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Abstract:The aim of this review was to explore the pharmacological activity of early tracheophytes (pteridophytes) as an alternative medicine for treating human ailments. As the first vascular plants, pteridophytes (aka, ferns and fern allies) are an ancient lineage, and human beings have been exploring and using taxa from this lineage for over 2000 years because of their beneficial properties. We have documented the medicinal uses of pteridophytes belonging to thirty different families. The lycophyte Selaginella sp. was shown in earlier studies to have multiple pharmacological activity, such as antioxidant, anti-inflammatory, anti-cancer, antidiabetic, antiviral, antimicrobial, and anti-Alzheimer properties. Among all the pteridophytes examined, taxa from the Pteridaceae, Polypodiaceae, and Adiantaceae exhibited significant medicinal activity. Based on our review, many pteridophytes have properties that could be used in alternative medicine for treatment of various human illnesses. Biotechnological tools can be used to preserve and even improve their bioactive molecules for the preparation of medicines against illness. Even though several studies have reported medicinal uses of ferns, the possible bioactive compounds of several pteridophytes have not been identified. Furthermore, their optimal dosage level and treatment strategies still need to be determined. Finally, the future direction of pteridophyte research is discussed.

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Isolation of Two Cytotoxic Diterpenes from the Fern Pteris multifida

Cited by 8 publications

References 0 publications

Structure -Cytotoxicity Relationships of a Series of Natural and Semi-Synthetic Simple Coumarins as Assessed in Two Human Tumour Cell Lines

Structure -Cytotoxicity Relationships of a Series of Natural and Semi-Synthetic Simple Coumarins as Assessed in Two Human Tumour Cell Lines

Potencial alelopático de espécies de Pteridaceae (Pteridophyta)

A review of the use of pteridophytes for treating human ailments

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