2005
DOI: 10.1111/j.1432-0436.2005.07302004.x
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Isolation of two human fibroblastic cell populations with multiple but distinct potential of mesenchymal differentiation by ceiling culture of mature fat cells from subcutaneous adipose tissue

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Cited by 63 publications
(67 citation statements)
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“…These results confirmed the findings that primary cells and their supernatants have similar potential for differentiation along the mesenchymal lineage to produce adipocytes, osteoblasts, and chondrocytes (Miyazaki et al 2005). In this context, the supernatant cells in the current experiment had functional properties closer to the DFAT cells reported by Buschmann et al (2013) rather than floating cells.…”
Section: First Induction Cycle Second Induction Cycle Third Inductionsupporting
confidence: 89%
“…These results confirmed the findings that primary cells and their supernatants have similar potential for differentiation along the mesenchymal lineage to produce adipocytes, osteoblasts, and chondrocytes (Miyazaki et al 2005). In this context, the supernatant cells in the current experiment had functional properties closer to the DFAT cells reported by Buschmann et al (2013) rather than floating cells.…”
Section: First Induction Cycle Second Induction Cycle Third Inductionsupporting
confidence: 89%
“…To achieve osteogenic differentiation in monolayer culture, LVEC cells were plated at an initial cell density of 5 PLLA/PLGA (PLLA: Poly-L-Lactic Acid; PLGA: poly(lacticco-glycolic acid)) scaffolds with pore size 200-400 lm were created by blending 50/50 PLLA and PLGA polymers having molecular weights 5000 and 75,000, respectively. The PLLA polymer was chosen to retard the high degradability of PLGA.…”
Section: Osteogenic Differentiationmentioning
confidence: 99%
“…These pluripotent stem cells have the capability to proliferate and self-renew over long periods of time and to differentiate toward almost all cell types. Mesenchymal stem cells (MSCs) may be isolated from a number of adult tissues, including bone marrow, fat, umbilical cord blood, and muscle [5][6][7][8][9][10], and have the capability to proliferate, differentiate, and form a number of mesenchymal tissues. Although MSCs are entering clinical trials for cardiac and orthopedic applications, these cells still have a limited expansion and differentiation capacity [11,12].…”
Section: Introductionmentioning
confidence: 99%
“…mature adipocyte fraction, is more tedious and complicated. Little information is available on this issue, and to the best of our knowledge, few attempts have been successful [11,22,23]. Very little data exists on the cell fraction present on the bottom of the ceiling cell culture of mature adipocyte fractions and only once has weak adipogenic differentiation been described for those cells [23].…”
Section: Introductionmentioning
confidence: 99%
“…Little information is available on this issue, and to the best of our knowledge, few attempts have been successful [11,22,23]. Very little data exists on the cell fraction present on the bottom of the ceiling cell culture of mature adipocyte fractions and only once has weak adipogenic differentiation been described for those cells [23]. Other authors report multipotent ADSC obtained from the mature adipocyte fraction by ceiling cell culture, although the cell population on the bottom of the flask was not investigated in the study [24].…”
Section: Introductionmentioning
confidence: 99%