Isolation of verotoxin-producing Escherichia coli O-rough:K1:H7 from two patients with traveler's diarrhea

Vilà, Jordi; Vargas, Martha; Ruiz, J.; Gallardo, Francisco Javier Flores; Anta, M. T. Jimenez De; Gascón, Joaquím

doi:10.1128/jcm.35.9.2279-2282.1997

Cited by 8 publications

(3 citation statements)

References 27 publications

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“…These strains of VTEC 01:H-have so far been isolated only in Europe from cases of diarrhoea, HUS, and from healthy carriers (Bockemtihl et al 1992;Bielaszewska et al 1994Bielaszewska et al , 1996Lehmacher et al 1998). Europe also seems to be the only source of VTEC 01 :H7 (Huppertz et al 1996;Karch et al 1997b;Vila et al 1997) and VTEC 01 :H20 (Pierard et al 1997), while Asia is the only source of 01:H1 (Tamura et al 1996).…”

Section: Serotypes Of Vtec Found Only In Human Diseasementioning

confidence: 99%

Role of non-O157 VTEC

Bettelheim

2000

Journal of Applied Microbiology

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SUMMARY Non‐O157 VTEC are typical Escherichia coli that differ only in their ability to produce verocytotoxins (VT). The transmission of VTEC is discussed in relation to the transmission of commensal E. coli. The emergence over the last few decades of a great variety of VTEC serotypes from healthy and diseased humans and animals is described. Particular attention is given to the distribution of the more important serogroups pathogenic for humans that have been described from around the world, particularly serogroups O26, O111, O128 and O103. The possible role of ruminants as reservoirs is discussed. The problems of laboratory diagnosis of non‐O157 VTEC are considered and various laboratory methods are assessed. Evidence is presented that the particular E. coli serotypes now known to be VTEC were present in humans and animals many years ago, but have acquired the ability to produce VT and probably other virulence factors. Finally, predictions are made of the possible increase in problems associated with these emerging pathogens.

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Section: Serotypes Of Vtec Found Only In Human Diseasementioning

confidence: 99%

Role of non-O157 VTEC

Bettelheim

2000

Journal of Applied Microbiology

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“…Four other publications reported a total of five isolates of STEC O117:H7 . Two were reported from Belgium in 1990 and 1997 ; one from Switzerland in 1992 and two isolates were reported from Spain in 1997 from patients with watery diarrhea after traveling in the Indian subcontinent and Central America .…”

Section: Chapter 4 Verotoxin/shiga Toxin‐producing (Vtec/stec) O117:mentioning

confidence: 99%

Characterization of four Escherichia coli clonal groups

Olesen

2017

APMIS

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(1991)(1992) that involved an unusual multi-resistant E. coli O78:H10 strain. Surprisingly, the isolates qualified as enteroaggregative E. coli (EAEC), a diarrheagenic pathotype, making this the first reported involvement of EAEC in an extraintestinal disease outbreak. Paper IV reports an analysis of 51 archived E. coli O78:H10 isolates (1956 2000) that showed E. coli O78:H10 to be clonally heterogeneous, comprising one dominant clonal group (61% of isolates, including all 19 Copenhagen outbreak isolates) from ST10 (phylogenetic group A), plus several minor clonal groups (phylogenetic groups A and D). The outbreak clone exhibited both extraintestinal pathogenic E. coli (ExPEC) and EAEC characteristics. Paper V reports evidence that the outbreak strain adhered more extensively to human bladder epithelial cells than did prototype uropathogenic (ExPEC) strains thanks to its aggregative adherence fimbriae, the principal adhesins of EAEC, and was capable of extensive biofilm formation on urethral catheters. These findings suggest that certain EAEC-specific virulence factors increase uropathogenicity.Paper VI reports a search for ST131, a globally disseminated, recently emerged multiresistant ExPEC clonal group, among 115 extended-spectrum beta-lactamase (ESBL)-producing E. coli clinical isolates from Danish patients (2008 -2009), to assess ST131's prevalence in Copenhagen among ESBL-producing isolates. ST131 was the most prevalent clonal group (38% of isolates) and compared with other ESBL isolates was more often community-associated and exhibited distinctive and extensive virulence profiles.Paper VII reports temporal trends in antimicrobial resistance and virulence genes among historical E. coli ST131 isolates (1968 -2011). Antimicrobial resistance increased over time due to emergence of the (resistance-associated) H30 and H30-Rx ST131 subclones. Distinctive characteristics of the ST131 H30-Rx subclone including specific virulence factors (iutA, afa/dra, kpsM II), K100 capsule, multidrug resistance, and ESBL production possibly contributed to this lineage's epidemiologic success. These findings confirm that ST131 represents a group of distinctive subclones, not a unitary entity.In conclusion, all four clonal groups were multiresistant and demonstrated the potential to spread locally or globally. A combination of antimicrobial resistance and specific virulence genes likely contributed to their epidemiologic success. Future implementation of rapid methods to screen for such multiresistant high-risk clones and their cardinal virulence genes conceivably could assist in clinical management and prevention efforts.

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“…The LPS profile of MA6 also lacks the O side chain and closely resembled that of DH5␣ pir; hence, it appears that MA6 is also a rough strain. Rough mutants of E. coli (O-rough:K1:H7) that produce Stx1 have been isolated from patients with traveler's diarrhea, but those strains were distinct phenotypically from O157:H7 and did not carry other EHEC virulence factors (16).…”

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confidence: 99%