Isolation of Yeasts from Various Food Products and Detection of Killer Toxin Activity &lt;i&gt;In vitro&lt;/i&gt;

Dubash, Taronish; Gupta, Saloni; Prakash, PY; Bairy, Indira

doi:10.3329/jsr.v2i2.4159

Cited by 7 publications

(6 citation statements)

References 6 publications

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“…Vitamins play key role in numerous metabolic processes of the body and yeasts have been reported to be able to produce vitamins, especially vitamin B complex, which is a distinctive advantage for yeast to be used as a probiotic over bacteria [52]. Dubash et al [53] reported a number of yeast strains belonging to Sachharomyces cerevisiae, Candida pintolopesii, Candida tropicalis, Pichia anomala and Dekkera spp. with killer toxin activity.…”

Section: Discussionmentioning

confidence: 99%

“…The isolate don’t produce gelatinase and DNase indicating its safety to be used for human patients as most of the pathogenic microorganisms produce these enzymes as part of their pathogenesis [26]. Cholesterol assimilation by yeast with probiotic attributes has also been reported by Chen et al [53]. Syal and Vohra [26] reported yeast isolates that showed high auto-aggregation ability and cell surface hydrophobicity.…”

Section: Discussionmentioning

confidence: 99%

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Antimicrobial and antioxidant activities of Saccharomyces cerevisiae IFST062013, a potential probiotic

Fakruddin

Hossain

Ahmed

2017

BMC Complement Altern Med

147

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BackgroundProbiotic yeast has become a field of interest to scientists in recent years.MethodsConventional cultural method was employed to isolate and identify yeast and standard methods were used to determine different probiotic attributes, antimicrobial and antioxidant properties.ResultsThis study reports potential probiotic properties of a strain of S. cerevisiae IFST 062013 isolated from fruit. The isolate is tolerant to a wide range of temperature and pH, high concentration of bile salt and NaCl, gastric juice, intestinal environment, α-amylase, trypsin and lysozyme. It can produce organic acid and showed resistance against tetracycline, ampicillin, gentamycin, penicillin, polymixin B and nalidixic acid. It can assimilate cholesterol, can produce killer toxin, vitamin B12, glutathione, siderophore and strong biofilm. It showed moderate auto-aggregation ability and cell surface hydrophobicity. The isolate can produce enzymes such as amylase, protease, lipase, cellulose, but unable to produce galactosidase. The isolate can’t produce gelatinase and DNase. The isolate showed moderate anti-microbial activity against bacteria and fungi and cell lysate showed better antimicrobial activity than whole cell and culture supernatant. Again, the isolate showed better anti-bacterial activity against gram negative bacteria than gram positive. The isolate showed strong antioxidant activity, reducing power, nitric oxide and hydroxyl radical scavenging activity, significant brine shrimp cytotoxicity and acute toxicity and metal ion chelating activity. The isolate did not induce any detectable change in general health of mice upon oral toxicity testing and found to be safe in mouse model. The isolate improve lymphocyte proliferation and cytokine production in treated mice.ConclusionsSuch isolate could be potential as probiotic to be used therapeutically.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Antimicrobial and antioxidant activities of Saccharomyces cerevisiae IFST062013, a potential probiotic

Fakruddin

Hossain

Ahmed

2017

BMC Complement Altern Med

147

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“…It must be taken into consideration that Candida is not only highly widespread in the human body but it is also the main genus of yeasts which contaminate foods [40], being C. albicans, C. parapsilosis, C. tropicalis, C. glabrata and C. rusei the main pathogenic species [41]. The first three of them have been frequently isolated from foods such as milk, cheese, juices and salads [42,43]. Regarding intra-yeast H. pylori detection in foods, it has been reported within yeasts species of Candida isolated from different food sources, such as yogurt, grape juice, bread, preserves, fruits and honey [30,39].…”

Section: Helicobacter Pylori and Its Relationship With Candida Spmentioning

confidence: 99%

Candida sp. As a Potential Reservoir and Transmission Facilitator of Helicobacter Pylori

Cancino¹

2018

BJSTR

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Helicobacter pylori is a bacterium broadly distributed in the world, colonizing nearly 50% of the human population. Its niche is the human stomach, where it is able to generate several gastric pathologies. A numbers of studies have detected Candida sp. together with H. pylori in the stomach, condition which could increase the severity of the gastric damage. Nevertheless, the association between these two microorganisms is closer because it has been reported that H. pylori can be found active within vacuoles of yeasts belonging to genus Candida. This association is present in yeasts isolated from diverse sources including foods as well as human bodies, such as mouth and vagina of pregnant women, suggesting the presence of a novel mechanism of protection and a means of transportation which facilitates H. pylori transmission. The aim of the present work is to review the relationship between H. pylori and yeasts of the genus Candida and the role of yeasts in the persistence of H. pylori worldwide infection.

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“…For primary testing, filtered or concentrated supernatant samples were dropped (10, 25, 50 lL) on plates with a 0 1 strain, and the formation of lysis zones was observed after 4 days of the incubation at 18-20°C. Concentrated proteins were analysed using SDS-PAGE method [25].…”

Section: Isolation Of Yeast Killer Toxins and Protein Electrophoresismentioning

confidence: 99%

A Novel Saccharomyces cerevisiae Killer Strain Secreting the X Factor Related to Killer Activity and Inhibition of S. cerevisiae K1, K2 and K28 Killer Toxins

et al. 2016

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It was determined that Kx strains secrete an X factor which can inhibit all known Saccharomyces cerevisiae killer toxins (K1, K2, K28) and some toxins of other yeast species-the phenomenon not yet described in the scientific literature. It was shown that Kx type yeast strains posess a killer phenotype producing small but clear lysis zones not only on the sensitive strain α'1 but also on the lawn of S. cerevisiae K1, K2 and K28 type killer strains at temperatures between 20 and 30 °C. The pH at which killer/antikiller effect of Kx strain reaches its maximum is about 5.0-5.2. The Kx yeast were identified as to belong to S. cerevisiae species. Another newly identified S. cerevisiae killer strain N1 has killer activity but shows no antikilller properties against standard K1, K2 and K28 killer toxins. The genetic basis for Kx killer/antikiller phenotype was associated with the presence of M-dsRNA which is bigger than M-dsRNA of standard S. cerevisiae K1, K2, K28 type killer strains. Killer and antikiller features should be encoded by dsRNA. The phenomenon of antikiller (inhibition) properties was observed against some killer toxins of other yeast species. The molecular weight of newly identified killer toxins which produces Kx type strains might be about 45 kDa.

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Isolation of Yeasts from Various Food Products and Detection of Killer Toxin Activity <i>In vitro</i>

Cited by 7 publications

References 6 publications

Antimicrobial and antioxidant activities of Saccharomyces cerevisiae IFST062013, a potential probiotic

Antimicrobial and antioxidant activities of Saccharomyces cerevisiae IFST062013, a potential probiotic

Candida sp. As a Potential Reservoir and Transmission Facilitator of Helicobacter Pylori

A Novel Saccharomyces cerevisiae Killer Strain Secreting the X Factor Related to Killer Activity and Inhibition of S. cerevisiae K1, K2 and K28 Killer Toxins

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