Isolation of Yersinia spp. from cases of diarrhoea in Iraqi infants and children

Kanan, T. A.; Abdulla, Zainalabideen A.

doi:10.26719/2009.15.2.276

Cited by 15 publications

(16 citation statements)

References 11 publications

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“…Yersiniosis has became the serious food-borne disease next to campylobacter and salmonella infection in developing countries of temperate zone [14,49]. Y. enterocolitica has been isolated from a broad range of food substances such as vegetables, meat, sea foods, poultry, egg products, dairy products etc.…”

Section: Resultsmentioning

confidence: 99%

Molecular evaluation of quorum quenching potential of vanillic acid against Yersinia enterocolitica through transcriptomic and in silico analysis

Sivasankar

Jha

Singh

et al. 2020

Journal of Medical Microbiology

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Introduction. Yersinia enterocolitica is one of the leading food-borne entero-pathogens causing various illnesses ranging from gastroenteritis to systemic infections. Quorum sensing (QS) is one of the prime mechanisms that control the virulence in Y. enterocolitica . Hypothesis/Gap Statement. Vanillic acid inhibits the quorum sensing and other virulence factors related to Y. enterocolitica . It has been evaluated by transcriptomic and Insilico analysis. Therefore, it can be a prospective agent to develop a therapeutic combination against Y. enterocolitica . Aim. The present study is focused on screening natural anti-quorum-sensing agents against Y. enterocolitica . The effect of selected active principle on various virulence factors was evaluated. Methodology. In total, 12 phytochemicals were screened by swarming assay. MATH assay, EPS and surfactant production assay, SEM analysis, antibiotic and blood sensitivity assay were performed to demonstrate the anti-virulence activity. Further, RNA sequencing and molecular docking studies were carried out to substantiate the anti-QS activity. Results. Vanillic acid (VA) has exhibited significant motility inhibition, thus indicating the anti-QS activity with MQIC of 400 µg ml−1 without altering the cell viability. It has also inhibited the violacein production in Chromobacterium violaceum ATCC 12472, which further confirms the anti-QS activity. VA has inhibited 16 % of cell-surface hydrophobicity (CSH), 52 % of EPS production and 60 % of surfactant production. Moreover, it has increased the sensitivity of Y. enterocolitica towards antibiotics. It has also made the cells upto 91 % more vulnerable towards human immune cells. The transcriptomic analysis by RNA sequencing revealed the down regulation of genes related to motility, virulence, chemotaxis, siderophores and drug resistance. VA treatment has also positively regulated the expression of several stress response genes. In furtherance, the anti-QS potential of VA has been validated with QS regulatory protein YenR by in silico molecular simulation and docking study. Conclusion. The present study is possibly the first attempt to demonstrate the anti-QS and anti-pathogenic potential of VA against Y. enterocolitica by transcriptomic and in silico analysis. It also deciphers that VA can be a promising lead to develop biopreservative and therapeutic regimens to treat Y. enterocolitica infections.

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Section: Resultsmentioning

confidence: 99%

Molecular evaluation of quorum quenching potential of vanillic acid against Yersinia enterocolitica through transcriptomic and in silico analysis

Sivasankar

Jha

Singh

et al. 2020

Journal of Medical Microbiology

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“…Many authors use PBS based cold enrichment protocols before Yersinia isolation obtaining satisfactory results [19]-[22], although no references were found in relation with their use before PCR. Cold enrichment with PBSMSB has been shown to be more effective than ITC for the recovery of Y. enterocolitica from pig tonsils, faeces and carcass swabs [18],[28].…”

Section: Discussionmentioning

confidence: 99%

“…But also with non-selective enrichments like phosphate-buffered saline broth (PBS), PBS supplemented with 1% sorbitol and 0.15% bile salt (PBSSSB) or PBS supplemented with 1% manitol and 0.15% bile salt (PBSMSB) [18]-[22]. …”

Section: Introductionmentioning

confidence: 99%

Evaluation of different enrichment methods for pathogenic Yersiniaspecies detection by real time PCR

et al. 2014

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BackgroundYersiniosis is a zoonotic disease reported worldwide. Culture and PCR based protocols are the most common used methods for detection of pathogenic Yersinia species in animal samples. PCR sensitivity could be increased by an initial enrichment step. This step is particularly useful in surveillance programs, where PCR is applied to samples from asymptomatic animals. The aim of this study was to evaluate the improvement in pathogenic Yersinia species detection using a suitable enrichment method prior to the real time PCR (rtPCR). Nine different enrichment protocols were evaluated including six different broth mediums (CASO, ITC, PSB, PBS, PBSMSB and PBSSSB).ResultsThe analysis of variance showed significant differences in Yersinia detection by rtPCR according to the enrichment protocol used. These differences were higher for Y. pseudotuberculosis than for Y. enterocolitica. In general, samples incubated at lower temperatures yielded the highest detection rates. The best results were obtained with PBSMSB and PBS2. Application of PBSMSB protocol to free-ranging wild board samples improved the detection of Y. enterocolitica by 21.2% when compared with direct rtPCR. Y. pseudotuberculosis detection was improved by 10.6% when results obtained by direct rtPCR and by PBSMSB enrichment before rtPCR were analyzed in combination.ConclusionsThe data obtained in the present study indicate a difference in Yersinia detection by rtPCR related to the enrichment protocol used, being PBSMSB enrichment during 15 days at 4°C and PBS during 7 days at 4°C the most efficient. The use of direct rtPCR in combination with PBSMSB enrichment prior to rtPCR resulted in an improvement in the detection rates of pathogenic Yersinia in wild boar and could be useful for application in other animal samples.

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“…In developing countries like Iraq [ 3 ], Iran [ 4 ], and Nigeria [ 5 ], the prevalence of gastrointestinal illness is highlighted including yersiniosis which highlights the major underlying food safety problems in low- and middle-income countries.…”

Section: Introductionmentioning

confidence: 99%

Detection ofYersinia enterocoliticain Retail Chicken Meat, Mashhad, Iran

Sirghani

Zeinali

Jamshidi

2018

Journal of Pathogens

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Poultry meat is one of the most important sources of infection of Yersinia spp. for humans. The aim of the present study was to evaluate the incidence of Yersinia enterocolitica in chicken meat by using culture method on selective medium and confirmation by PCR assay. Also, biochemical methods were used for biotyping. A total of 100 chicken thigh meat samples were collected randomly from retail outlets in Mashhad, Iran. Samples were enriched in Peptone-Sorbitol-Bile (PSB) broth and then cultured on Cefsulodin-Irgasan-Novobiocin (CIN) agar containing antibiotics supplement. The DNA was extracted from suspected colonies of Yersinia spp. and then PCR test using specific primers for 16S rRNA gene of Yersinia enterocolitica was performed. In this study, 30% of chicken meat was contaminated with Yersinia spp. by culture method and 25% of chicken meat was contaminated with Yersinia enterocolitica. Biotyping of isolated colonies showed that all of the isolates belonged to biotype 1A. Culture and detection of Yersinia spp. from food samples traditionally take 4 days. Due to high accuracy and speed of PCR assay, it is a good alternative method for microbiological techniques. In conclusion, poultry meat can act as a source of Y. enterocolitica and could be considered as a public health hazard.

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Isolation of Yersinia spp. from cases of diarrhoea in Iraqi infants and children

Cited by 15 publications

References 11 publications

Molecular evaluation of quorum quenching potential of vanillic acid against Yersinia enterocolitica through transcriptomic and in silico analysis

Molecular evaluation of quorum quenching potential of vanillic acid against Yersinia enterocolitica through transcriptomic and in silico analysis

Evaluation of different enrichment methods for pathogenic Yersiniaspecies detection by real time PCR

Detection ofYersinia enterocoliticain Retail Chicken Meat, Mashhad, Iran

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