Isolation of α-Glucuronidase Enzyme from a Rumen Metagenomic Library

Lee, Charles C.; Kibblewhite, Rena E.; Wagschal, Kurt; Li, Ruiping; Orts, William J.

doi:10.1007/s10930-012-9391-z

Cited by 11 publications

(4 citation statements)

References 37 publications

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“…Our results indicate that most proteins encoded by microbial genes identified for the prediction of FCR were generally involved in carbohydrates metabolism and transport. For example, aguA and K01188 are involved in biomass conversion, through the degradation of hemicelluloses and lignocelluloses and lactate biosynthesis (Cairns and Esen, 2010; Lee et al, 2012; Michlmayr and Kneifel, 2014; Li, 2015). Microbial genes xylE , aguA , and uidA are involved in xylan degradation, the main component of hemicellulose (Lee et al, 2012; Fliegerova et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…For example, aguA and K01188 are involved in biomass conversion, through the degradation of hemicelluloses and lignocelluloses and lactate biosynthesis (Cairns and Esen, 2010; Lee et al, 2012; Michlmayr and Kneifel, 2014; Li, 2015). Microbial genes xylE , aguA , and uidA are involved in xylan degradation, the main component of hemicellulose (Lee et al, 2012; Fliegerova et al, 2015). Xylose needs to be taken up by a transporter (putatively associated with xylE ) before it is metabolized, and it has been recognized as a rate-controlling step in bacterial metabolism (Chaillou and Pouwels, 1999).…”

Section: Discussionmentioning

confidence: 99%

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Identification of Rumen Microbial Genes Involved in Pathways Linked to Appetite, Growth, and Feed Conversion Efficiency in Cattle

et al. 2019

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The rumen microbiome is essential for the biological processes involved in the conversion of feed into nutrients that can be utilized by the host animal. In the present research, the influence of the rumen microbiome on feed conversion efficiency, growth rate, and appetite of beef cattle was investigated using metagenomic data. Our aim was to explore the associations between microbial genes and functional pathways, to shed light on the influence of bacterial enzyme expression on host phenotypes. Two groups of cattle were selected on the basis of their high and low feed conversion ratio. Microbial DNA was extracted from rumen samples, and the relative abundances of microbial genes were determined via shotgun metagenomic sequencing. Using partial least squares analyses, we identified sets of 20, 14, 17, and 18 microbial genes whose relative abundances explained 63, 65, 66, and 73% of the variation of feed conversion efficiency, average daily weight gain, residual feed intake, and daily feed intake, respectively. The microbial genes associated with each of these traits were mostly different, but highly correlated traits such as feed conversion ratio and growth rate showed some overlapping genes. Consistent with this result, distinct clusters of a coabundance network were enriched with microbial genes identified to be related with feed conversion ratio and growth rate or daily feed intake and residual feed intake. Microbial genes encoding for proteins related to cell wall biosynthesis, hemicellulose, and cellulose degradation and host–microbiome crosstalk (e.g., aguA, ptb , K01188, and murD ) were associated with feed conversion ratio and/or average daily gain. Genes related to vitamin B12 biosynthesis, environmental information processing, and bacterial mobility (e.g., cobD , tolC , and fliN ) were associated with residual feed intake and/or daily feed intake. This research highlights the association of the microbiome with feed conversion processes, influencing growth rate and appetite, and it emphasizes the opportunity to use relative abundances of microbial genes in the prediction of these performance traits, with potential implementation in animal breeding programs and dietary interventions.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Identification of Rumen Microbial Genes Involved in Pathways Linked to Appetite, Growth, and Feed Conversion Efficiency in Cattle

et al. 2019

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“…Prevotella and Bacteroides were the predominant genera harboring the enzyme families GH31 ( Prevotella : ∼70%, Bacteroides : ∼11%), GH36 ( Prevotella : ∼48%, Bacteroides : ∼8%), and GH27 ( Prevotella : ∼50%, Bacteroides : ∼14%). Alpha-glucuronidases play an essential role in the complete hydrolysis of hemicellulose and were studied in Bacteroides vulgaris and Dysgonomonas mossii ( Lee et al, 2012 ). The enzyme family GH67 was linked to the genera Chryseobacterium (∼29%), Dysgonomonas (∼10%) and Paludibacter (∼8%).…”

Section: Discussionmentioning

confidence: 99%

The Planktonic Core Microbiome and Core Functions in the Cattle Rumen by Next Generation Sequencing

Wirth

Kádár²,

Kakuk

et al. 2018

Front. Microbiol.

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The cow rumen harbors a great variety of diverse microbes, which form a complex, organized community. Understanding the behavior of this multifarious network is crucial in improving ruminant nutrient use efficiency. The aim of this study was to expand our knowledge by examining 10 Holstein dairy cow rumen fluid fraction whole metagenome and transcriptome datasets. DNA and mRNA sequence data, generated by Ion Torrent, was subjected to quality control and filtering before analysis for core elements. The taxonomic core microbiome consisted of 48 genera belonging to Bacteria (47) and Archaea (1). The genus Prevotella predominated the planktonic core community. Core functional groups were identified using co-occurrence analysis and resulted in 587 genes, from which 62 could be assigned to metabolic functions. Although this was a minimal functional core, it revealed key enzymes participating in various metabolic processes. A diverse and rich collection of enzymes involved in carbohydrate metabolism and other functions were identified. Transcripts coding for enzymes active in methanogenesis made up 1% of the core functions. The genera associated with the core enzyme functions were also identified. Linking genera to functions showed that the main metabolic pathways are primarily provided by Bacteria and several genera may serve as a “back-up” team for the central functions. The key actors in most essential metabolic routes belong to the genus Prevotella. Confirming earlier studies, the genus Methanobrevibacter carries out the overwhelming majority of rumen methanogenesis and therefore methane emission mitigation seems conceivable via targeting the hydrogenotrophic methanogenesis.

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“…These xylanases cleave the substrate to produce short xylooligosaccharides that are substituted at the non-reducing end with a (4Me)-glucuronic acid moiety [44]. A GH67 α-glucuronidase (Agu) enzyme was used because this enzyme has been demonstrated to remove (4Me)-glucuronic acid from these xylooligosaccharide fragments [45]. Our previous work with these two specific enzymes had demonstrated that they act synergistically to release the (4Me)-glucuronic acid moiety from the substrate.…”

Section: Rosettazyme Assemblymentioning

confidence: 99%

Production of D-Xylonic Acid from Hemicellulose Using Artificial Enzyme Complexes

Lee¹,

Kibblewhite²,

Paavola³

et al. 2017

Journal of Microbiology and Biotechnology

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Lignocellulosic biomass represents a potentially large resource to supply the world's fuel and chemical feedstocks. Enzymatic bioconversion of this substrate offers a reliable strategy for accessing this material under mild reaction conditions. Owing to the complex nature of lignocellulose, many different enzymatic activities are required to function in concert to perform efficient transformation. In nature, large multienzyme complexes are known to effectively hydrolyze lignocellulose into constituent monomeric sugars. We created artificial complexes of enzymes, called rosettazymes, in order to hydrolyze glucuronoxylan, a common lignocellulose component, into its cognate sugar -xylose and then further convert the-xylose into -xylonic acid, a Department of Energy top-30 platform chemical. Four different types of enzymes (endoxylanase, α-glucuronidase, β-xylosidase, and xylose dehydrogenase) were incorporated into the artificial complexes. We demonstrated that tethering our enzymes in a complex resulted in significantly more activity (up to 71%) than the same amount of enzymes free in solution. We also determined that varying the enzyme composition affected the level of complex-related activity enhancement as well as overall yield.

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Isolation of α-Glucuronidase Enzyme from a Rumen Metagenomic Library

Cited by 11 publications

References 37 publications

Identification of Rumen Microbial Genes Involved in Pathways Linked to Appetite, Growth, and Feed Conversion Efficiency in Cattle

Identification of Rumen Microbial Genes Involved in Pathways Linked to Appetite, Growth, and Feed Conversion Efficiency in Cattle

The Planktonic Core Microbiome and Core Functions in the Cattle Rumen by Next Generation Sequencing

Production of D-Xylonic Acid from Hemicellulose Using Artificial Enzyme Complexes

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