Isolation, Optimisation and Purification of Lipase Production by Pseudomonas Aeruginosa

Zouaoui, Benattouche; Abbouni, Bouziane

doi:10.4172/2155-952x.1000120

Cited by 19 publications

(16 citation statements)

References 18 publications

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“…3). This was in agreement with Zouaoui et al [53] who reported olive oil as the best lipase inducer in Pseudomonas aeruginosa and also correlates with the findings by Benjamin and Pandey [54] who showed that Candida rugosa lipase production was proportionally increased with the increase in concentration of olive oil and maximum production was achieved at 10% (v/v) olive oil concentration. Enzyme production with relatively high activity is possible only after optimizing growth parameters.…”

Section: Resultssupporting

confidence: 92%

“…6) and this was followed closely by lactose. Lactose was reported as best carbon source for lipase production by Rhizopus MR12 [56], glucose as best carbon source for Rhizopus JK-1 [53] and dextrose as best for Pseudomonas aeruginosa [51]. Among the various organic and inorganic nitrogen sources tested, maximum lipase production (29.43 U/ml) was observed with yeast extract (Fig.…”

Section: Resultsmentioning

confidence: 89%

“…This greater efficiency in the hydrolysis of oils is because there is increased reactant mobility, increased miscibility of the lipids and other hydrophobic substrates in water and also because higher temperatures hasten reaction rates [62]. Rhizopus MR12 [56] and Rhizopus strain JK-1 [53] both produced lipase optimally at 30 o C. Both medium pH and temperature affect microbial lipase fermentations. Our isolate Rhizopus oryzae ZAC3 can grow in the pH range of 4.0 -8.0 and temperature range of 25 -55 o C. Maximum enzyme production was obtained at 45 o C, although the culture was able to grow even at a higher temperature of 55 o C while still retaining some considerable activity.…”

Section: Resultsmentioning

confidence: 99%

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Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Ayinla¹,

Ademakinwa²,

Agboola³

2017

J App Biol Biotech

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This study investigated the screening, production and optimization of an extracellular lipase from a fungus isolated from the contaminated soil of a palm oil processing shed. This was with a view to obtaining a strain that can secrete lipase with biochemical properties exploitable for biotechnological applications such as bioremediation of oil contaminated sites. Soil samples were collected from palm oil contaminated sites in Gbogan, Osun State, Nigeria (Latitude N 7°29.1481´ and Longitude E 4°20.7587´). The isolated fungal strains were screened on tributyrin agar for exogenous lipolytic activity. Molecular identification was carried out by amplifications of ITS-1, 5.8S and ITS-2 regions. The effects of incubation time, inducers, pH, temperature, carbon and nitrogen sources were varied for optimal lipase production using one factor at a time approach. Rhizopus oryzae ZAC3 (NCBI accession No: KX035094) was identified as the highest lipaseproducing strain. Maximum lipase production was observed on the fourth day, pH 5.0 and a temperature of 45 o C. Olive oil, xylose and yeast extract were the best inducer, carbon and nitrogen sources respectively for lipase production. There was a 2.02 fold increase in lipase production under these optimized conditions. In conclusion, Rhizopus oryzae ZAC3 lipase has properties exploitable for industrial and biotechnological applications.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 99%

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Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Ayinla¹,

Ademakinwa²,

Agboola³

2017

J App Biol Biotech

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“…Diameters of lipases production by P. aeruginosa on tween 80 agar in this study were rather closed to those reported by Zouaoui et al (2012) who found that P. aeruginosa isolates when grown on tween 80 agar gave hydrolyzing zone diameters ranging between 5 to 12 mm.…”

Section: Detection Of Virulence Factorssupporting

confidence: 83%

Effect of Probiotics on Some Virulence Factors of Pseudomonas Aeruginosa Isolated From Clinical Samples

Jaleel¹

2017

WJPR

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This study aimed to detect the inhibitory effect of some probiotics against pyocyanin and lipases activity produced by Pseudomonas aeruginosa, and comparing this effect with that of the commonly used antibiotics. From a total of 209 bacterial isolates collected from different clinical sources (Urine, ear, sputum, congenital disorder fluids, pus, and wounds) of two hospitals in Baghdad, 16 of them were identified as P. aeruginosa. When the sixteen isolates were examined for pyocyanin and lipases production on glycerol supplemented nutrient agar and tween 80 agar, respectively, all isolates, except one, were positive for pigment and enzymes production. Four probiotic microorganisms namely (Lactobacillus acidophilus, Lb. fermentum, Lb. plantarum and Saccharomyces boulardii) were propagated in their media. After incubation, their fermented aliquots were centrifuged and filtered which were considered as unconcentrated filtrates. From these filtrates, one, two and three-fold concentrated filtrates were prepared. Antibiotic solutions of Amikacin, Ceftazidime, Imipenem and Tobramycin were also prepared at a concentration of 10μg/ml for comparison. Results revealed that the three-fold concentrated filtrate of Lb. acidophilus was able to inhibit pyocyanin activity strongly (almost entirely) and lipases moderately, followed by those filtrates of Lb. fermentum and Lb. plantarum which were less strongly in their inhibition. On the other hand, the three-fold filtrate of S. boulardii was weakly effective in this regard. Regarding the antibiotics used in this study, Ceftazidime was the most effective one, followed by Imipenem and Tobramycin with moderate effect, then Amikacin with the least effect, while neither Imipenem nor Tobramycin were unable to show any effect on lipases produced by P. aeruginosa. From the results, it can concluded that among all

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“…According to Qamsari et al (2011), Zn 2+ and Cu 2+ salts also decreased activity by 32 and 27%, respectively. Also, Zouaoui and Bouziane (2011) (Kumar and Kanwar, 2011).…”

Section: Effect Of Ph On Lipase Activitymentioning

confidence: 99%

Characterization and optimization of lipase activity produced by Pseudomonas monteilli 2403-KY120354 isolated from ground beef

Rasmey¹,

Aboseidah²,

Gaber³

et al. 2017

Afr. J. Biotechnol.

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A total of 56 Gram negative bacterial isolates were recovered from twenty ground beef samples and were screened for their potentiality to produce lipase. Forty four bacterial isolates were recorded as positive producers for lipase on tween as carbon source in solid medium. Also, the highly producer isolates were screened for lipase activity in submerged culture using olive oil as carbon and the most active isolate was 2043 which gave an activity of 20.0 ± 0.29 U/ml. The bacterial isolate 2403 was identified phenotypically according to Bergey's Manual and genotypically using 16S rRNA genes analysis as Pseudomonas monteilli. Effect of some different factors on lipase activity were studied and the maximum lipase activity was achieved at reaction medium of pH 6 and incubated at 40°C for 60 min. Also, addition of Ba 2+ in the reaction medium enhanced the lipase activity, while the other tested metals reduced the enzyme activity.

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Isolation, Optimisation and Purification of Lipase Production by Pseudomonas Aeruginosa

Cited by 19 publications

References 18 publications

Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Effect of Probiotics on Some Virulence Factors of Pseudomonas Aeruginosa Isolated From Clinical Samples

Characterization and optimization of lipase activity produced by Pseudomonas monteilli 2403-KY120354 isolated from ground beef

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