Isolation, Optimization of Fermentation Conditions, and Characterization of an Exopolysaccharide from Pseudoalteromonas agarivorans Hao 2018

Hao, Lujiang; Liu, Wenlin; Liu, Kai; Shan, Kai; Xi, Chenxiang; Liu, Jinfeng; Fan, Qiuping; Zhang, Xiaofei; Lü, Xiaoping; Xu, Yanrui; Cao, Ruiwen; Ma, Yong; Zheng, Lan; Cui, Bo

doi:10.3390/md17120703

Cited by 19 publications

(17 citation statements)

References 40 publications

(38 reference statements)

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“…This increase in PMM expression is accompanied by a decrease in PGM expression levels, thereby increasing the conversion of glucose-6-P to mannose-1-P, ultimately leading to increased GDP-l-fucose production in the strain (Ju et al, 2022). EPS from P. agarivorans have previously been reported to exhibit good antioxidant activity (Hao et al, 2018). PGM has recently been shown to play a major role in xyloglucan catabolism in the anaerobic Gram-positive bacterium Ruminiclostridium cellulolyticum, where it converts…”

Section: Discussionmentioning

confidence: 99%

Isolation, identification, and biochemical characterization of a novel bifunctional phosphomannomutase/phosphoglucomutase from the metagenome of the brown alga Laminaria digitata

et al. 2022

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Macroalgae host diverse epiphytic bacterial communities with potential symbiotic roles including important roles influencing morphogenesis and growth of the host, nutrient exchange, and protection of the host from pathogens. Macroalgal cell wall structures, exudates, and intra-cellular environments possess numerous complex and valuable carbohydrates such as cellulose, hemi-cellulose, mannans, alginates, fucoidans, and laminarin. Bacterial colonizers of macroalgae are important carbon cyclers, acquiring nutrition from living macroalgae and also from decaying macroalgae. Seaweed epiphytic communities are a rich source of diverse carbohydrate-active enzymes which may have useful applications in industrial bioprocessing. With this in mind, we constructed a large insert fosmid clone library from the metagenome of Laminaria digitata (Ochrophyta) in which decay was induced. Subsequent sequencing of a fosmid clone insert revealed the presence of a gene encoding a bifunctional phosphomannomutase/phosphoglucomutase (PMM/PGM) enzyme 10L6AlgC, closely related to a protein from the halophilic marine bacterium, Cobetia sp. 10L6AlgC was subsequently heterologously expressed in Escherichia coli and biochemically characterized. The enzyme was found to possess both PMM and PGM activity, which had temperature and pH optima of 45°C and 8.0, respectively; for both activities. The PMM activity had a Km of 2.229 mM and Vmax of 29.35 mM min−1 mg−1, while the PGM activity had a Km of 0.5314 mM and a Vmax of 644.7 mM min−1 mg−1. Overall characterization of the enzyme including the above parameters as well as the influence of various divalent cations on these activities revealed that 10L6AlgC has a unique biochemical profile when compared to previously characterized PMM/PGM bifunctional enzymes. Thus 10L6AlgC may find utility in enzyme-based production of biochemicals with different potential industrial applications, in which other bacterial PMM/PGMs have previously been used such as in the production of low-calorie sweeteners in the food industry.

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Section: Discussionmentioning

confidence: 99%

Isolation, identification, and biochemical characterization of a novel bifunctional phosphomannomutase/phosphoglucomutase from the metagenome of the brown alga Laminaria digitata

et al. 2022

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“…The EPS in deionized water was further purified by using DEAE-52 anion-exchange chromatography and gel filtration chromatography [50]. Then, the purified EPS was dialyzed with deionized water and selective semipermeable membranes (8000~14,400 da) [17]. The polysaccharides were freeze-dried and stored at 4 • C, and labeled as P7, P8, and P9, respectively.…”

Section: Extraction and Purification Of Epss Under Different Fermentation Conditionsmentioning

confidence: 99%

“…In addition, EPSs from marine bacteria have marked anti-tumor, antiviral, and immunomodulatory functions [15]. Previous studies have shown that the exopolysaccharide produced by P. agarivorans Hao 2018 has good antioxidant activity, hygroscopicity, and moisture retention, and also has good absorption of Cu 2+ and Pb 2+ [16,17].…”

Section: Introductionmentioning

confidence: 99%

“…In this study, the EPS-producing marine bacterium P. agarivorans was derived from the microbial membrane on the surface of abalone seedlings. Based on the study by Hao et al, the strain P. agarivorans Hao 2018 was cultured by changing the initial pH of the fermentation, and then EPSs were obtained by extraction, isolation, and purification to study the effect of environmental disturbances on EPS [17,25]. The monosaccharide composition of EPS was analyzed via high-performance liquid chromatography (HPLC) and Fourier transform infrared (FT-IR) spectroscopy.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Different Initial Fermentation pH on Exopolysaccharides Produced by Pseudoalteromonas agarivorans Hao 2018 and Identification of Key Genes Involved in Exopolysaccharide Synthesis via Transcriptome Analysis

Shan

Liu

et al. 2022

Marine Drugs

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Exopolysaccharides (EPSs) are carbohydrate polymers produced and secreted by microorganisms. In a changing marine environment, EPS secretion can reduce damage from external environmental disturbances to microorganisms. Meanwhile, EPSs have promising application prospects in the fields of food, cosmetics, and pharmaceuticals. Changes in external environmental pH have been shown to affect the synthesis of EPSs in microorganisms. In this study, we analyzed the effects of different initial fermentation pHs on the production, monosaccharide composition, and antioxidant activity of the EPSs of Pseudoalteromonas agarivorans Hao 2018. In addition, the transcriptome sequence of P. agarivorans Hao 2018 under different initial fermentation pH levels was determined. GO and KEGG analyses showed that the differentially expressed genes were concentrated in the two-component regulatory system and bacterial chemotaxis pathways. We further identified the expression of key genes involved in EPS synthesis during pH changes. In particular, the expression of genes encoding the glucose/galactose MFS transporter, phosphomannomutase, and mannose-1-phosphate guanylyltransferase was upregulated when the environmental pH increased, thus promoting EPS synthesis. This study not only contributes to elucidating the environmental adaptation mechanisms of P. agarivorans, but also provides important theoretical guidance for the directed development of new products using biologically active polysaccharides.

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“…The carbohydrate content of EPS from P. agarivorans stain Hao 2018 (yield 4.5 g/L) isolated from Yellow Sea of China contained 90% glucose and 6% mannose. The main biological activity of this strain was moisture retention and absorption of free radicals (i.e., antioxidant) with potential applications in food and cosmetics industries (Hao et al, 2019 ). Pseudoalteromonas sp.…”

Section: Marine Biosurfactant-producing Bacteriamentioning

confidence: 99%

Biosurfactants and Their Applications in the Oil and Gas Industry: Current State of Knowledge and Future Perspectives

Nikolova

Gutiérrez

2021

Front. Bioeng. Biotechnol.

112

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Surfactants are a group of amphiphilic chemical compounds (i.e., having both hydrophobic and hydrophilic domains) that form an indispensable component in almost every sector of modern industry. Their significance is evidenced from the enormous volumes that are used and wide diversity of applications they are used in, ranging from food and beverage, agriculture, public health, healthcare/medicine, textiles, and bioremediation. A major drive in recent decades has been toward the discovery of surfactants from biological/natural sources—namely bio-surfactants—as most surfactants that are used today for industrial applications are synthetically-manufactured via organo-chemical synthesis using petrochemicals as precursors. This is problematic, not only because they are derived from non-renewable resources, but also because of their environmental incompatibility and potential toxicological effects to humans and other organisms. This is timely as one of today's key challenges is to reduce our reliance on fossil fuels (oil, coal, gas) and to move toward using renewable and sustainable sources. Considering the enormous genetic diversity that microorganisms possess, they offer considerable promise in producing novel types of biosurfactants for replacing those that are produced from organo-chemical synthesis, and the marine environment offers enormous potential in this respect. In this review, we begin with an overview of the different types of microbial-produced biosurfactants and their applications. The remainder of this review discusses the current state of knowledge and trends in the usage of biosurfactants by the Oil and Gas industry for enhancing oil recovery from exhausted oil fields and as dispersants for combatting oil spills.

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Isolation, Optimization of Fermentation Conditions, and Characterization of an Exopolysaccharide from Pseudoalteromonas agarivorans Hao 2018

Cited by 19 publications

References 40 publications

Isolation, identification, and biochemical characterization of a novel bifunctional phosphomannomutase/phosphoglucomutase from the metagenome of the brown alga Laminaria digitata

Isolation, identification, and biochemical characterization of a novel bifunctional phosphomannomutase/phosphoglucomutase from the metagenome of the brown alga Laminaria digitata

Effect of Different Initial Fermentation pH on Exopolysaccharides Produced by Pseudoalteromonas agarivorans Hao 2018 and Identification of Key Genes Involved in Exopolysaccharide Synthesis via Transcriptome Analysis

Biosurfactants and Their Applications in the Oil and Gas Industry: Current State of Knowledge and Future Perspectives

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