Isolation, phenotypic characterization and comparative genomic analysis of 2019SD1, a polyvalent enterobacteria phage

Kumar, Prince; Meghvansi, Mukesh K.; Kamboj, Dev Vrat

doi:10.1038/s41598-021-01419-8

Cited by 7 publications

(10 citation statements)

References 71 publications

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“…When the ten phages were assayed at 50°C, they showed, at most, a 1-log 10 PFU ml −1 reduction after 1 h, indicating a high resistance for all phages studied (Figure 1a). Similar results were obtained by other authors when they evaluated resistance of S. flexneri (Mallick et al, 2021;Zhang et al, 2012) and S. dysenteriae (Kumar et al, 2021) phages at 40 and 50°C. In contrast, another phage lytic against S. flexneri was almost completely inactivated at 50°C (Xu et al, 2021).…”

Section: Influence Of Temperature On Viability and Activity Of Phagessupporting

confidence: 90%

“…Furthermore, they found an approximately 16% increment in vB_SflS‐ISF001 activity after incubation in 1 g/dL of salt solution (Shahin & Bouzari, 2018). On the other hand, phage viability was significantly affected at 2 g dL −1 of Na + for phage 2019SD1 (Kumar et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

“…Although many authors have studied phage viability at various physicochemical conditions such as thermal treatments (Jamal et al, 2015; Xu et al, 2021), varying pH (Mallick et al, 2021; Zhang et al, 2012), and salt concentrations (Kumar et al, 2021; Shahin & Bouzari, 2018), the activity of phage/ Shigella systems has never been evaluated in physicochemical conditions similar to those found in food matrices. Moreover, even though many phage–host studies have been previously performed, those focusing on Shigella strains have been scarcely documented.…”

Section: Introductionmentioning

confidence: 99%

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Physicochemical characterization of ten newly isolated phages against the foodborne pathogen Shigella flexneri

Tomat

González

Aquili

et al. 2022

Food Processing Preservation

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Ten bacteriophages with lytic activity against Shigella flexneri were tested for their resistance to physicochemical conditions found in food matrices. Phage viability and activity were evaluated at different temperatures, pH values, and NaCl concentrations.In addition, challenge tests with each individual phage against ATCC 12022 were conducted to evaluate their use as biocontrol agents. Although phages showed high resistance up to 60°C, Shi30, Shi33, and Shi93 showed higher thermal resistance (70°C).The ten phages endured pH treatments, being more resistant to alkaline conditions. The NaCl concentrations evaluated showed no significant influence on phage counts. Furthermore, challenge tests against ATCC 12022 resulted in significant CFU ml −1 reductions. The results showed that the phages evaluated were resistant to a wide range of temperatures and pH and to all the concentrations of NaCl tested. Therefore, the phages evaluated in this study could potentially be used with biocontrol purposes in various food matrices prior further characterization.Novelty impact statement: Ten newly isolated phages against the foodborne pathogen Shigella flexneri remained viable after different physicochemical treatments. Furthermore, their activity was maintained after thermal and chemical treatments. Also, the ten phages were effective against pathogenic Shigella flexneri strains in challenge tests.

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Section: Influence Of Temperature On Viability and Activity Of Phagessupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Physicochemical characterization of ten newly isolated phages against the foodborne pathogen Shigella flexneri

Tomat

González

Aquili

et al. 2022

Food Processing Preservation

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“…The incubation and outbreak periods of phage PaVOA were 10 and 20 min, respectively. Compared to phage 2019SD1 isolated by Kumar (Kumar et al, 2021) and phage SMP isolated by Ma and Lu (Ma and Lu, 2008), the incubation and outbreak periods of phage PaVOA were shorter, indicating that the phage could infect bacteria more rapidly, thus reflecting the high efficiency of PaVOA. Moreover, during the growth phase of PaVOA, the titer can be increased by the addition of calcium and magnesium ions.…”

Section: Discussionmentioning

confidence: 93%

Topically applied bacteriophage to control multi-drug resistant Pseudomonas aeruginosa-infected wounds in a New Zealand rabbit model

Wang

Meng²,

Zhang³

et al. 2022

Front. Microbiol.

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Pseudomonas aeruginosa (P. aeruginosa) is a widespread, gram-negative, pathogenic bacterium that causes serious internal and external infections in humans and other animals. The increasing antibiotic resistance has complicated bacterial infection treatment, and current antibiotic therapies cannot cure all infections. Owing to this, bacteriophages (phages) have regained attention as potential therapeutics for bacterial infections. In this study, the phage “PaVOA” was isolated from hospital sewage and characterized. Next, a New Zealand rabbit skin infection model was used to determine the therapeutic efficacy of PaVOA as compared to a phage cocktail or the cephalosporin antibiotic ceftriaxone. Characterization results demonstrated that phage PaVOA belongs to the Myoviridae family, has a double-stranded DNA genome, is resistant to low temperatures (−20°C), is most optimal at 40°C, has good acid–base tolerance, and remains stable for 30 min under 20 W ultraviolet (UV) intensity. The optimal multiplicity of infection of PaVOA was 0.1, and a one-step growth curve showed a short latency period (10 min), thus demonstrating its ability to rapidly kill bacteria. Furthermore, the addition of calcium (Ca) and magnesium (Mg) ions significantly increased the PaVOA titer. An in vivo phage kinetic curve showed that PaVOA was rapidly inactivated within the blood of New Zealand rabbits (undetectable after 12 h), and no animals died due to phage treatment. Wound healing studies showed that the phage cocktail induced a high healing rate and an acceleration of the skin remodeling process, and was more efficacious than ceftriaxone. Therefore, phage cocktail therapy represents a novel therapeutic approach in the treatment of traumatic skin infections caused by multi-drug resistant P. aeruginosa.

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“…Following the manufacturer’s instructions, DNAse I, RNAse A, and mung bean nuclease (Takara Biomedical Technology Co., Ltd., Beijing, China) were used to confirm the type of nucleic acid in the phage (Kumar et al 2021 ).…”

Section: Methodsmentioning

confidence: 99%

Characteristics of a novel temperate bacteriophage against Staphylococcus arlettae (vB_SarS_BM31)

et al. 2022

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Background Staphylococcus arlettae is a rarely reported coagulase-negative staphylococcus (CoNS) isolated from infected humans and livestock. Observing phage-bacteria interaction could improve the understanding of bacterial pathogenetic mechanisms, providing foundational evidence for phage therapy or phage detection. Herein, we aimed to characterise and annotate a novel bacteriophage, vB_SarS_BM31 (BM31), specific to S. arlettae . This bacteriophage was isolated from a milk sample associated with bovine mastitis and collected in the Sichuan Province, China. Results The BM31 genome comprised a linear double-stranded DNA of 42,271 base pair in length with a G + C content of 34.59%. A total of 65 open reading frames (ORFs) were assembled from phage DNA, of which 29 were functionally annotated. These functional genes were divided into four modules: the structural, DNA packing and replication, lysis, and lysogeny modules. Holin (ORF25), lysin (ORF26), and integrase (ORF28) were located closely in the entire BM31 genome and were important for lyse or lysogeny cycle of BM31. The phage was identified as a temperate phage according to whole genome analysis and life cycle assay, with basic biological characteristics such as small burst size, short latency period, and narrow host range, consistent with the characteristics of the family Siphoviridae, subcluster B14 of the Staphylococcus bacteriophage. Conclusions The present isolation and characterisation of BM31 contributes to the Staphylococcus bacteriophage database and provides a theoretical foundation for its potential applications. To the best of our knowledge, BM31 is the only shared and completely reported phage against S. arlettae in the entire public database. Supplementary Information The online version contains supplementary material available at 10.1007/s10123-022-00292-3.

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Isolation, phenotypic characterization and comparative genomic analysis of 2019SD1, a polyvalent enterobacteria phage

Cited by 7 publications

References 71 publications

Physicochemical characterization of ten newly isolated phages against the foodborne pathogen Shigella flexneri

Physicochemical characterization of ten newly isolated phages against the foodborne pathogen Shigella flexneri

Topically applied bacteriophage to control multi-drug resistant Pseudomonas aeruginosa-infected wounds in a New Zealand rabbit model

Characteristics of a novel temperate bacteriophage against Staphylococcus arlettae (vB_SarS_BM31)

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