Isolation, Purification and Characterization of Boeravinone B From Boerhaavia Diffusa Linn.

Abstract

Boerhavia diffusa is a medicinal plant used in treatment of autoimmune diseases, cancers and inflammation. In the present study, methanolic extraction of roots of Boerhaavia diffusa was carried out and presence of Boeravinone B was identified from Thin Layer Chromatography and reverse phase HPLC. The methanolic root extract was run in TLC containing the mobile phase as toluene: ethyl acetate: methanol in the ratio (7:1:2). Retention factor for Boeravinone B in B.diffusa extract was found to be 0.461. RP-HPLC was carried out from the mobile phase consisting of acetonitrile and water at the ratio of 50:50 at 35 °C with a flow rate of 1.0 mL/min in C18 reverse phase column. Retention time of Boeravinone B was found to be 13.8 min. Linearity range of the concentration of extracted Boeravinone B was 5 -120 μg/ml and the correlation coefficient (R 2 ) value was found to be 0.997±0.003. Recovery was found to be 90.6% -92.8%. The limit of detection (LOD) of Boeravinone B was found to be 2 μg/ml and limit of quantitation (LOQ) was found to be 5 μg/ml. Presence of functional groups such as phenyl groups at 1450.47 cm -1 , 1591.27 cm -1 , C=C (Carbon skeleton) at 1618.28 cm -1 and Isoflavonoid structure at 1649.14 cm -1 of absorption spectra of isolated Boeravinone B were characterized by FTIR spectroscopy through recording absorption spectra. Thus, a rapid, economical and viable method was developed for isolation, purification and characterization of Boeravinone B.