Isolation, Purification and Characterization of Antioxidative Bioactive Elastin Peptides from Poultry Skin

Abstract

Muscle-based by-products are often undervalued although commonly reported having a high amount of natural bioactive peptides. In this study, elastin was isolated from the protein of broiler hen skin while its hydrolysate was prepared using Elastase. Assessment of antioxidative properties of elastin-based hydrolysate (EBH) was based on three different assays; 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical, 2,2'-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical and metal chelating ability. The EBH was purified further using ultrafiltration, gel filtration and Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC). The IC50 of ABTS radical activities for EBH were decreased as EBH further purified using ultrafiltration (EBH III; 0.66 mg/mL)>gel filtration (EB-II; 0.42 mg/mL)>RP-HPLC (EB-II4; 0.12 mg/mL). The sequential identification of the peptide was done by matrix-assisted laser desorption/ ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/ TOF-MS) of the potent fractions obtained from RP-HPLC (EB-II4). The presence of hydrophobic amino acids (Val and Pro) in the peptide sequences could potentially contribute to the high antioxidant activity of EBH. The sequences GAHTGPRKPFKPR, GMPGFDVR and ADASVLPK were identified as antioxidant peptides. In conclusion, the antioxidative potential from poultry skin specifically from elastin is evident and can be explored to be used in many applications such as health and pharmaceutical purposes.

Characterization and Toxicity Evaluation of Broiler Skin Elastin for Potential Functional Biomaterial in Tissue Engineering

“…The ratio of isoleucine/leucine was 0.56, which is in range of 0.35–0.60 as mentioned before. These results are in line and have similar patterns with commercial elastin as reported by Yusop et al [ 9 ] and other elastin peptides from previous studies [ 19 , 20 ]. The detection of high levels of methionine and histidine is related to fibrils and microfibril residues of collagen [ 39 ].…”

“…To confirm the quality of the extracted elastin, chemical-grade commercial elastin (Sigma-Aldrich, St. Louis, MO, USA), harvested from bovine neck ligament, was used as a benchmark. The extraction of elastin from broiler skin was performed using the method reported by Nadalian et al [ 20 ] and Kamaruzaman et al [ 32 ] with some modification. First, visible fat on broiler skin was removed manually after being thawed.…”

“…Elastin from broiler chicken skin by-product was first isolated by Nadalian et al [ 19 ]. Several consecutive studies reported the presence of its peptides with antioxidative qualities and as a potential ingredient for cosmetics formulation [ 9 , 20 ]. Understanding elastin’s potential features and applications from various species requires knowledge of its characteristics and bioactive capabilities.…”

Tannic acid: a versatile polyphenol for design of biomedical hydrogels

“…162,163 Thus, oxidizing TA could be a promising approach for the hydrogel formation with amino group-containing biopolymers to attain high mechanical stability due to the strong covalent bonds in addition to TA's multiple physical interactions. A wide range of polymers such as gum Arabic, 159 chitosan, 164 alginate, 165 and polyphosphate 155 have been incorporated into the formulation of the gelatin–TA-based hydrogels.…”

In-situ oligomerization of lactic acid within broiler skin extracted elastin/collagen matrix for the efficacy of ointment base

“…Collagen and elastin are potentially found in different parts of animals and plants forming a complex matrix with other organic molecules [ 21 ]. Due to this, the extraction and isolation become more intricate which further affect the cost [ 33 ]. Therefore, finding a cheaper raw material with high throughput and an easier alternative extraction method is a primary concern.…”