Isolation, purification, and characterization of a novel exopolysaccharide isolated from marine bacteria Brevibacillus borstelensis M42

Srivastava, Nandita; Kumari, Sumeeta; Kurmi, Shubham; Pinnaka, Anil Kumar; Choudhury, Anirban Roy

doi:10.1007/s00203-022-02993-9

Cited by 11 publications

(3 citation statements)

References 65 publications

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“…Its surface exhibited a dense, smooth, and porous morphology, displaying superior thermal stability, water-holding capacity, and oil-holding capacity compared to many other EPS. The exceptional water-holding capacity of EPS Br42, reaching 510% [ 23 ], can be attributed to its porous microstructure, glycosidic bonds, and high polymerization degree. Therefore, it can be inferred that the heightened polymerization degree of DT1-0 may contribute to its superior water-holding capacity.…”

Section: Discussionmentioning

confidence: 99%

Evaluate the Structural and Physicochemical Properties of Exopolysaccharides Produced by Bacillus halotolerans Isolated from Locally Sourced Vegetables

Dai,

Xu,

Zhou

et al. 2024

Polymers

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In this study, a Bacillus halotolerans (B. halotolerans) strain DT1 capable of producing exopolysaccharides (EPS) was isolated from dried cabbages of Tianjin, a local fermented vegetable product. Three distinct polysaccharide fractions were isolated from the fermentation broth of DT1, namely, DT1-0, DT1-2, and DT1-5. The structural composition and properties of these fractions were investigated. The predominant EPS, DT1-0, was identified as a novel heteropolysaccharide composed of fructose and glucose with branched structures. The repeating unit was determined to be [4)-α-D-Glcp-(1→6)-α-D-Glcp-(1→6)-β-D-Fruf-(2→6)-β-D-Fruf-(2→6-)-β-D-Fruf-(2→], with fructose and glucose connected by β-(2→1) and α-(1→4) glycosidic linkages between the third fructose and the first glucose, respectively. The molecular weight (Mw) was estimated to be 4.253 × 103 Da. DT1-0 presented a smooth and porous surface structure as observed through SEM and exhibited a water-holding capacity of 504 ± 5.3%, maximum thermal stability at 245 °C, and an oil-holding capacity of 387 ± 1.9% for coconut oil. DT1-2 was identified as a fructooligosaccharide. DT1-5 was characterized as a polysaccharide composed of glucose and fructose. In conclusion, these findings provide substantial support for the further application of B. subtilis strain DT1 and its EPS fractions, DT1-0, DT1-2, and DT1-5, as potential alternatives for functional food additives or ingredients.

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Section: Discussionmentioning

confidence: 99%

Evaluate the Structural and Physicochemical Properties of Exopolysaccharides Produced by Bacillus halotolerans Isolated from Locally Sourced Vegetables

Dai,

Xu,

Zhou

et al. 2024

Polymers

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“…Therefore, there is an increased likelihood of identifying novel microorganisms, which, in turn, may lead to the isolation of novel polysaccharides with unique properties, especially since some taxa can produce only specific polymers [4]. For instance, in India, Srivastava et al [23] isolated a novel heteropolysaccharide of around 286 kDa, made up of glucose and galacturonic acid, from the marine bacterium Brevibacillus borstelensis, while in a different study, Rhodotorula mucilaginosa, a marine-derived red yeast, was found to yield a new 1200 KDa exopolysaccharide that consisted of fucose, galactose, mannose, and glucose [24]. Similarly, there are also reports on the isolation of novel exopolysaccharide-producing species or other new marine-derived polymers [25][26][27][28][29].…”

Section: The Case Of Marine Polysaccharidesmentioning

confidence: 99%

Marine Microbial Polysaccharides: An Untapped Resource for Biotechnological Applications

et al. 2023

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As the largest habitat on Earth, the marine environment harbors various microorganisms of biotechnological potential. Indeed, microbial compounds, especially polysaccharides from marine species, have been attracting much attention for their applications within the medical, pharmaceutical, food, and other industries, with such interest largely stemming from the extensive structural and functional diversity displayed by these natural polymers. At the same time, the extreme conditions within the aquatic ecosystem (e.g., temperature, pH, salinity) may not only induce microorganisms to develop a unique metabolism but may also increase the likelihood of isolating novel polysaccharides with previously unreported characteristics. However, despite their potential, only a few microbial polysaccharides have actually reached the market, with even fewer being of marine origin. Through a synthesis of relevant literature, this review seeks to provide an overview of marine microbial polysaccharides, including their unique characteristics. In particular, their suitability for specific biotechnological applications and recent progress made will be highlighted before discussing the challenges that currently limit their study as well as their potential for wider applications. It is expected that this review will help to guide future research in the field of microbial polysaccharides, especially those of marine origin.

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“…A polysaccharide produced by a microbe and secreted outside its cells is known as an exopolysaccharide (EPS). 24 Curdlan is one such EPS isolated from Alcaligenes faecalis var. myxogenes 10C 3 in 1962 by Harada and colleagues for the first time.…”

Section: Microbial Polysaccharidesmentioning

confidence: 99%

Microbial Polysaccharide-Based Nanoformulations for Nutraceutical Delivery

Srivastava

Choudhury

2022

ACS Omega

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In recent times, nutrition and diet have become prominent health paradigms due to sedentary lifestyle disorders. Preventive health care strategies are becoming increasingly popular instead of treating and managing diseases. A nutraceutical is an innovative concept that offers additional health benefits beyond its fundamental nutritional value. These nutraceuticals have the potential to reduce the exorbitant use of synthetic drugs because the modern medicine approach of treating diseases with high-tech, expensive supplements, and long-term consequences aggravates consumers. However, most nutraceuticals are plant-derived, making them susceptible to degradation and prone to chemical instability, poor solubility, unpleasant taste, and bioactivity loss before absorption to the targeted site. To counteract this problem, the bioavailability of these labile compounds can be maximized by encapsulating them in protective nanocarriers. It is crucial that nanoencapsulation technologies convert bioactive compounds into forms that can be easily combined with functional foods and beverages without adversely affecting their organoleptic properties. In recent years, nanoformulations using food-grade materials, such as polysaccharides, proteins, lipids, etc., have received considerable attention. Among them, microbial polysaccharides are biocompatible, nontoxic, and nonimmunogenic, and most of them are US-FDA approved and can undergo tailored modifications. The nanoformulation of microbial polysaccharide is a relatively new frontier which has several advantages over existing systems. The present article, for the first time, comprehensively reviews microbial polysaccharides-based nanodelivery systems for nutraceuticals and discusses various techno-commercial aspects of these nanotechnological preparations. Moreover, this has also attempted to draw a future research perspective in this area.

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Isolation, purification, and characterization of a novel exopolysaccharide isolated from marine bacteria Brevibacillus borstelensis M42

Cited by 11 publications

References 65 publications

Evaluate the Structural and Physicochemical Properties of Exopolysaccharides Produced by Bacillus halotolerans Isolated from Locally Sourced Vegetables

Evaluate the Structural and Physicochemical Properties of Exopolysaccharides Produced by Bacillus halotolerans Isolated from Locally Sourced Vegetables

Marine Microbial Polysaccharides: An Untapped Resource for Biotechnological Applications

Microbial Polysaccharide-Based Nanoformulations for Nutraceutical Delivery

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