Isolation, purification and characterization of peroxidase from Raphanus sativus and its applications in biotransformation of cresols

Saikia, Shilpa; Gogoi, Rahul Deep; Yadav, Meera; Yadav, Hardeo Singh

doi:10.1016/j.bcab.2022.102540

Cited by 8 publications

(6 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The optimal temperature of activity of peroxidase A6 is higher than those obtained for the major part of other peroxidases. Indeed, several peroxidases have their optimal temperature of activity ranging between 30 and 50 °C [4,5,6]. This property of peroxidase A6 is a great advantage on the practical level, for the rate of enzyme catalysis generally increases with an increase in temperature until a critical point known as the optimum temperature beyond which denaturation of the enzyme is initiated and the reaction rate begins to decrease.…”

Section: Optimal Temperaturementioning

confidence: 99%

“…However, the majority of enzymes become unstable at high temperatures. While most peroxidases that have been investigated thus far exhibit optimal activity in the range of 30 to 50 °C [4,5,6], very few of them can withstand exposure to those temperatures for extended periods. Additionally, horseradish peroxidase, whose heat stability is weak, is currently the main peroxidase that is commercially accessible.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Purification and partial characterization of a thermostable peroxidase isoenzyme from Bambara groundnut (Vigna subterranea L. Verdc) seedlings

Yves Mann Elate Lea Mbassi,

Marie-Solange Evehe Bebandoue,

Wilfred Fon Mbacham

2024

GSC Biol. Pharm. Sci.

View full text Add to dashboard Cite

Thermostable isoperoxidases, especially peroxidase A6, have already been found in seedlings of Vigna sp, indicating their potential for biotechnological applications. In this study, peroxidase A6 was purified from the roots of Bambara groundnut (Vigna subterranea L. Verdc) seedlings by a combination of gel filtration on Sephadex G-100, heat treatment, and ion exchange chromatography on CM cellulose and DEAE cellulose. The 41-kDa enzyme shows high catalytic efficiency in the oxidation of O-dianisidine, ABTS, TMB, DAB and OPD at acidic pH optimum and the reduction of H2O2. It has an optimum temperature of activity of 60 °C and a calculated activation energy of 221.5 KJ/mol for its thermal inactivation at pH 8. Mg2+ inhibits the enzyme. Ca2+ strongly increases its thermal stability, while Mn2+ and Zn2+ decrease it. The enzyme is inhibited by sodium azide at concentrations above 1 µM, with an IC50 value of about 10 µM. This inhibition, in addition to the RZ value of 2.4 (A403nm/A280nm), confirms the presence of a haem group in the active site, which is common for class III plant peroxidases. Due to its unique catalytic and thermal properties, peroxidase A6 has the potential to be a valuable tool for various biotechnological applications, especially for enzyme immunoassays and clinical diagnosis.

show abstract

Section: Optimal Temperaturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Purification and partial characterization of a thermostable peroxidase isoenzyme from Bambara groundnut (Vigna subterranea L. Verdc) seedlings

Yves Mann Elate Lea Mbassi,

Marie-Solange Evehe Bebandoue,

Wilfred Fon Mbacham

2024

GSC Biol. Pharm. Sci.

View full text Add to dashboard Cite

show abstract

“…are heme-containing enzymes produced from organisms such as plants, animals, and bacteria, and they are in the oxidoreductase enzyme class. 13 It has been reported that 87% of heme-containing peroxidases are not of animal origin, and these peroxidases are divided into three classes: class I, II, and III. Peroxidases in class I are effective in clearing and eliminating intracellular hydrogen peroxide.…”

Section: Introductionmentioning

confidence: 99%

Horseradish Peroxidase Immobilized onto Mesoporous Magnetic Hybrid Nanoflowers for Enzymatic Decolorization of Textile Dyes: A Highly Robust Bioreactor and Boosted Enzyme Stability

Bakar,

Akbulut,

Ulusal

et al. 2024

ACS Omega

View full text Add to dashboard Cite

Recently, hybrid nanoflowers (hNFs), which are accepted as popular carrier supports in the development of enzyme immobilization strategies, have attracted much attention. In this study, the horseradish peroxidase (HRP) was immobilized to mesoporous magnetic Fe3O4–NH2 by forming Schiff base compounds and the HRP@Fe3O4–NH2/hNFs were then synthesized. Under optimal conditions, 95.0% of the available HRP was immobilized on the Fe3O4–NH2/hNFs. Structural morphology and characterization of synthesized HRP@Fe3O4–NH2/hNFs were investigated. The results demonstrated that the average size of HRP@Fe3O4–NH2/hNFs was determined to be around 220 nm. The ζ-potential and magnetic saturation values of HRP@Fe3O4–NH2/hNFs were −33.58 mV and ∼30 emu/g, respectively. Additionally, the optimum pH, optimum temperature, thermal stability, kinetic parameters, reusability, and storage stability were examined. It was observed that the optimum pH value shifted from 5.0 to pH 8.0 after immobilization, while the optimum temperature shifted from 30 to 80 °C. K m values were calculated to be 15.5502 and 7.6707 mM for free HRP and the HRP@Fe3O4–NH2/hNFs, respectively, and V max values were calculated to be 0.0701 and 0.0038 mM min–1. The low K m value observed after immobilization indicated that the affinity of HRP for its substrate increased. The HRP@Fe3O4–NH2/hNFs showed higher thermal stability than free HRP, and its residual activity after six usage cycles was approximately 45%. While free HRP lost all of its activity within 120 min at 65 °C, the HRP@Fe3O4–NH2/hNFs retained almost all of its activity during the 6 h incubation period at 80 °C. Most importantly, the HRP@Fe3O4–NH2/hNFs demonstrated good potential efficiency for the biodegradation of methyl orange, phenol red, and methylene blue dyes. The HRP@Fe3O4–NH2/hNFs were used for a total of 8 cycles to degrade methyl orange, phenol red, and methylene blue, and degradation of around 81, 96, and 56% was obtained in 8 h, respectively. Overall, we believe that the HRP@Fe3O4–NH2/hNFs reported in this work can be potentially used in various industrial and environmental applications, particularly for the biodegradation of recalcitrant compounds, such as textile dyes.

show abstract

“…Diversification of agriculture is part of economic growth. This process involves a gradual transition from subsistence crops to a diversified, demand-based production system through fast technological change, improved rural infrastructure for agricultural production, and changing consumption patterns [17,18]. The benefits of agricultural diversification include increased income for farmers, improved food security, and reduced vulnerability to climate change.…”

Section: Introductionmentioning

confidence: 99%

Agricultural diversification and intra-household dietary diversity: Panel data analysis of farm households in Bangladesh

Alam,

Begum,

Mastura

et al. 2023

PLoS ONE

View full text Add to dashboard Cite

This paper investigates the associations between agricultural diversification and dietary diversity among men, women and children of farm households in Bangladesh. Using three waves of nationally representative Bangladesh Integrated Household Survey (BIHS- 2011–12, 2015 and 2018) data and a panel data model, the study found that agricultural production diversification can emphasize the dietary diversification across the life cycle of farm household members. The balanced panel data reveals that agricultural production diversification has a statistically significant and positive impact on dietary diversity of individual members (men, women and children) of farm households. Moreover, other important factors that impact on agricultural diversification and improve dietary diversity like women’s education and employment, commercialization of farm households, access to non-farm income sources, and access to information facilities also have a strong association in improving dietary status, food and nutritional security as well. Although there is currently limited diversity in the diets of adult men, women and children of rural farm households, the situation can be improved over time with proper and consistent support. The findings suggest policy interventions should target not only agricultural diversification but also improve women’s education and on and off farm employment opportunities and facilitate better access to information for rural households. These recommendations can support improved dietary diversity for all household members under different settings.

show abstract

Isolation, purification and characterization of peroxidase from Raphanus sativus and its applications in biotransformation of cresols

Cited by 8 publications

References 30 publications

Purification and partial characterization of a thermostable peroxidase isoenzyme from Bambara groundnut (Vigna subterranea L. Verdc) seedlings

Purification and partial characterization of a thermostable peroxidase isoenzyme from Bambara groundnut (Vigna subterranea L. Verdc) seedlings

Horseradish Peroxidase Immobilized onto Mesoporous Magnetic Hybrid Nanoflowers for Enzymatic Decolorization of Textile Dyes: A Highly Robust Bioreactor and Boosted Enzyme Stability

Agricultural diversification and intra-household dietary diversity: Panel data analysis of farm households in Bangladesh

Contact Info

Product

Resources

About