Isolation, purification, and structural elucidation of polysaccharides from Alhagi-honey

Abstract

The polysaccharide extract (PE) of Uyghur medicinal preparation Alhagi-honey was prepared by water extraction and alcohol precipitation method. The purified polysaccharide AP1-1 was obtained from PE by macroporous adsorption resin chromatography, DEAE cellulose chromatography, and Sephadex gel chromatography; the homogeneity and the molecular weight of AP1-1 were determined by gel filtration; and the acid hydrolysis, periodate oxidation, Smith degradation, and NMR analysis were used to analyze the chemical structure of AP1-1. The result showed that AP1-1 was a homogeneous polysaccharide, whose relative molecular weight was 9.97 × 10(4). Through high-performance capillary electrophoresis analysis, we found that its molecular structure was composed of mannose, glucose, galactose, and galacturonic acid with a molar ratio of about 1.1:1.9:3.9:2.1. The main chain of AP1-1 was mainly made up of → 4)β-d-GalpA-(1 → 4)β-d-GalpA-(1 → 4)-β-d-Galp-(1 → 4)-β-d-Galp-(1 → 6)α-d-Glcp-(1 → 4)α-d-Glcp(1 → , while the side chain is composed of → 6)-α-d-Glcp and 2-CH3-α-d-Man.

Isolation, purification, structural analysis and coagulatory activity of water-soluble polysaccharides from Ligustrum lucidum Ait flowers

“…Previous studies have shown that the strong biological activity of polysaccharides was strongly related to monosaccharide compositions [ 28 ], and the monosaccharide composition of polysaccharides played an important role in further analyzing its physicochemical properties, structure and structure-biological activity. At present, there were many ways to determine the monosaccharide composition, including high performance liquid chromatography [ 29 ], reversed-phase high performance liquid chromatography (HPLC) after pre-column derivatization [ 30 ], high-performance thin-layer chromatography [ 31 ], gas chromatography (GC) [ 32 ], high-performance anion-exchange chromatography [ 33 ], high performance capillary electrophoresis [ 34 ]. In our study, the monosaccharide compositions were measured by GC with good sensitivity, and monosaccharide composition was estimated by comparing retention time (RT).…”

<p>Cationic polymer modified PLGA nanoparticles encapsulating Alhagi honey polysaccharides as a vaccine delivery system for ovalbumin to improve immune responses</p>

“…AHP was extracted and purified using a previously described preparative column chromatography method,27 AHP [MW 47,479 Da, main chain-2,3)‒α‒L-Rhap‒(1,2,6)α‒D‒Manp‒(1,3)‒β‒D‒Galp-(1,3,6) α‒D-Galp‒ (1-, while the side chain is composed of α-D‒Glcp‒(1‒] carbohydrate and protein contents were 99.0% and 0.7%, respectively. OVA and AHP-loaded PLGA nanoparticles (AHPP/OVA) were prepared using a water/oil/water (w/o/w) double emulsion technique 28.…”

Preliminary Characterization of a Homogeneous Polysaccharide with Anticomplement Activity from Sijunzi Decoction

“…Moreover, the ratio of the amount of monosaccharide to amount of periodate was nearly 1 : 1 for polysaccharide S-3-1. This proved that the 1,3-linked forms that do not consume periodate were either not present or few in number [28]. The periodateoxidized products of S-3-1 were further reduced, hydrolyzed, and analyzed by GC-MS.…”