Isolation, purification, characterization and immunostimulatory activity of polysaccharides derived from American ginseng

Yu, Xin; Liu, Ying; Wu, Xian-Ling; Liu, Li-Zhai; Fu, Wei; Song, Dandan

doi:10.1016/j.carbpol.2016.08.092

Cited by 140 publications

(85 citation statements)

References 38 publications

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“…The extraction solution was deproteinized by the Sevag method (Pan, 2015) repeated three times. The activated carbon powder was used for decolorization and the operation was repeated several times until no color remains (Yu et al, 2017). The dialysis bags were used to remove small molecule impurities (including monosaccharides, oligosaccharides and some salt ions, etc).…”

Section: Preparation Of Polysaccharide From E Angustifoliamentioning

confidence: 99%

Hypoglycemic potential of Elaeagnus angustifolia fruit polysaccharide on streptozotocin-induced type 2 diabetic mice

Wang

Hong

Cui

et al. 2018

Bangladesh J Pharmacol

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In this study, the hyperglycemic potential of Elaeagnus angustifolia fruit polysaccharide in both normal healthy and streptozotocin-induced diabetic mice was investigated. Results showed no significant effect of E. angustifolia fruit polysaccharide on blood glucose level in normal control group, while E. angustifolia significantly suppressed the rise in blood glucose of diabetic mice. In addition, in the first two weeks of administration, the body weight was decreased both in negative control group and E. angustifolia groups, however, E. angustifolia (800 mg/kg) was recovered to the began weight in the fourth week. E. angustifolia (800 mg/kg) could markedly reduce the levels of total cholesterol, triglyceride and improve the level of high density lipoprotein-cholesterol. The results suggest that E. angustifolia could be considered as an ingredient of functional foods for diabetes.Video Clip of Methodology:10 min 19 sec <a href="https://www.youtube.com/v/a3ikXeeByP8">Full Screen</a> <a href="https://www.youtube.com/watch?v=a3ikXeeByP8">Alternate</a>

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Section: Preparation Of Polysaccharide From E Angustifoliamentioning

confidence: 99%

Hypoglycemic potential of Elaeagnus angustifolia fruit polysaccharide on streptozotocin-induced type 2 diabetic mice

Wang

Hong

Cui

et al. 2018

Bangladesh J Pharmacol

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“…As shown in Figure , the broad intense band at 3,398 cm −1 was attributed to the stretching vibrations of O―H. The weak band around 2,938 cm −1 displayed the C―H stretching and the bending vibrations of CH 2 or CH 3 (Yu et al., ). A relatively strong absorption peak occurred at 1,641 cm −1 owing to the carboxylate ion stretching band (COO − ; Coimbra, Barros, Rutledge, & Delgadillo, ).…”

Section: Resultsmentioning

confidence: 98%

A Mixture of Functional Complex Extracts from Lycium barbarum and Grape Seed Enhances Immunity Synergistically In Vitro and In Vivo

You

Chang

Zhao

et al. 2019

Journal of Food Science

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A mixture of multiple ingredients is often more effective than the individual ingredients. The functions of Lycium barbarum polysaccharide (LBP) glycoconjugate and grape seed procyanidins (GSP) are widely known. Here, we investigated the synergistic immune‐enhancing activity of LBP and GSP. Atomic force microscopy results suggested that the mixture of LBP and GSP exhibited circular structure unlike LBP alone, and the addition of polyphenols may change the spatial conformation of the sugar chain. The changes in the structure were related to the synergistic effect of the two functional agents on immune recovery. In vitro, the proliferation rate of splenocytes was higher in LBP + GSP group (64.16%), rather than the sum of LBP group (13.01%) and GSP group (43.61%) individually used. This synergistical proliferation of splenocytes may be correlated to the increasing intracellular free calcium levels. Furthermore, the mixture significantly enhanced the immunity in vivo, as evident from the recovery of peripheral white blood cell counts in LBP + GSP group (18.535 × 109/L) to normal group levels (18.115 × 109/L) and higher B cell proliferation than normal group (P < 0.05). These results highlight the immune‐enhancing activity of the combination of LBP and GSP associated with the structural changes, which may facilitate the development of functional foods with fewer resources but enhanced activities. Practical Application The synergistic effects of LBP and GSP on immunomodulatory were better than the sum of the effects of the individual agents both in vitro and in vivo. Our results may provide a research‐based support for the development of related functional products and an insight into the production of food resources with a fewer but more effective functional agents for better results.

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“…According to the characteristic absorption peaks of each functional group in the molecule, its structure can be analyzed and identified. As shown in Figure , the characteristic absorption peak of GYT‐1, GYT‐2, and GYT‐3 in infrared spectra of about 3100–3500 cm −1 , 1870–1650 cm −1 , and 1400–1200 cm −1 was corresponding to the stretching vibration of O‐H, C=O and the angular vibration of C‐H, respectively (Jahanbin, Abbasian, & Ahang, ; Xu et al, ; Yu et al, ). The signal of 1200–1000 cm −1 was assigned to the stretching vibration of C‐O‐C, indicating the presence of pyranose ring (Rozi et al, ).…”

Section: Resultsmentioning

confidence: 99%

Extraction, purification, characterization, anticoagulant activity, and anticoagulant mechanism of polysaccharides from the heads of Hypomesus olidus

Zhang

et al. 2019

Food Science & Nutrition

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The aim of this work was to extract, isolate, and purify polysaccharides from the heads of Hypomesus olidus and to evaluate their anticoagulant activities and anticoagulant mechanisms. Response surface methodology was used to optimize the extraction conditions for ultrasonic‐assisted extraction of polysaccharides from the heads of Hypomesus olidus. The optimal extraction conditions consisted of ultrasonic power of 275 W, ultrasonic time of 50 min, and solid–liquid ratio of 5 ml/g, giving the yield of crude polysaccharides (GYT) of 7.73 ± 0.042%. Three polysaccharide fractions, GYT‐1, GYT‐2, and GYT‐3 were purified from GYT by using DEAE‐cellulose‐52 column and Sephadex G‐100 column for anticoagulant activities. The results showed that two doses (2 and 4 mg/ml) of GYT‐1 and GYT‐3 could significantly prolong (p < .01) in partial thromboplastin time (APTT) (2.19 and 2.37 times, 2.22 and 2.44 times, respectively) and thrombin time (TT) (2.39 and 2.46 times, 2.44 and 2.80 times, respectively) compared with normal control. In particular, GYT‐3 had stronger anticoagulant activity than GYT‐1, and it was composed of arabinose, fructose, glucose, and lactose with molar ratios of 0.595:1: 2.026:0.273. However, GYT‐2 had no anticoagulant activity (p > .05). In addition, anticoagulation mechanism of polysaccharides from the heads of Hypomesus olidus (GYT‐3) was evaluated. The results showed that the anticoagulant activity of GYT‐3 was based on their binding with antithrombin AT‐III. And the inhibitory effects of GYT‐3 on factor IIa and Xa were related to the concentration of AT‐III in plasma. This study may provide a new and promising anticoagulant drug.

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Isolation, purification, characterization and immunostimulatory activity of polysaccharides derived from American ginseng

Cited by 140 publications

References 38 publications

Hypoglycemic potential of Elaeagnus angustifolia fruit polysaccharide on streptozotocin-induced type 2 diabetic mice

Hypoglycemic potential of Elaeagnus angustifolia fruit polysaccharide on streptozotocin-induced type 2 diabetic mice

A Mixture of Functional Complex Extracts from Lycium barbarum and Grape Seed Enhances Immunity Synergistically In Vitro and In Vivo

Extraction, purification, characterization, anticoagulant activity, and anticoagulant mechanism of polysaccharides from the heads of Hypomesus olidus

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