Isolation, Screening and Antibiotic Sensitivity of Pseudomonas species from Kelana Jaya Lake Soil in Selangor Malaysia

Abstract

Pathogenic microorganisms from hospitals, communities, and the environment remain great threats to human health. The increasing concern about antibiotic resistance has also necessitated the search for robust alternatives. Therefore, this study aims to isolate, screen and evaluate the antibiotic susceptibility of Pseudomonas aeruginosa isolated from a soil sample taken from northern, western and eastern parts of Kelana Jaya Lake against four antibiotics (gentamycin, tetracycline, ampicillin, and penicillin) on a Mueller-Hinton Agar media plate. Pseudomonas identification was done by using API 20 kit. Disc diffusion was employed as well as the oxidase test. From the positive oxidase result, the isolated bacteria were identified as Burkholderia cepacia (97.6% ID), Pseudomonas aeruginosa (99.5-99.9% ID), and Pseudomonas fluorescent (75.9% ID). Only Pseudomonas aeruginosa isolates were further evaluated for antibiotic susceptibility tests. The result showed that P. aeruginosa was susceptible to only three antibiotics (gentamycin, tetracycline, and penicillin) showing a clear zone of inhibition while it was resistant to only ampicillin with no zone of inhibition. Soil isolates are potential sources for the development of effective antibiotics against resistant bacteria.

تثبيط تكوين الاغشية الحيوية في بكتيريا Agrobacterium tumefaciens بالمادة الطافية الخالية من خلايا Pseudomonas aeruginosa والمحللة بجهاز GC-MS

“…In several articles, in-depth research identified specific chemicals in cell-free supernatants that have anti-QS activity 17 or alter the surface characteristics of bacterial cells 18 . On the other hand, the use of microorganisms in the field of biotechnology is one of the most prominent scientific developments used to protect economically and medically important plants from pathogens of all kinds 19 , and in this context, many bacterial species were used, including the genus Pseudomonas, mainly isolated from the soil , where it is located near the roots of plants and releases metabolites that promote plant growth and prevent the activity of pathogenic microbes 20,21 . As well as its positive effect in reducing the levels of toxic chemicals and heavy metals in the soil 22,23 .…”

Influence pH on virulence genes of Pseudomonas aeruginosa analyzed by RT-PCR method

“…P. aeruginosa is responsible for about 10% of all nosocomial bacterial infections, and as a result, 25-60% of patients pass away (Kumar, Paliya, & Singh, 2022). In terms of antibiotic resistance, the genus Pseudomonas is diverse and one of the most virulent pathogens (Ahmed, Aljondi, Alabed, Al-Mahdi, & Abdsalam, 2021). In roughly 65% of infections, bacteria develop as biofilms, which attach to and spread across the surface of plants (roots) or animals (epithelium).…”

Molecular study of the relationship of gene expression of some genes with the temperature variation of bacterial growth

“…In humans, P. aeruginosa is an opportunistic pathogen responsible for serious infections 3 . It is the major bacterial pathogen that colonizes cystic fibrosis patients and is one of the most common infectious agents in nosocomial infections, along with severe burn, cancer, transplantation and acquired immunodeficiency syndrome(AIDS) 4 .Also in the hospital, it is responsible for the formation of biofilms on the medical device surfaces 5 .Several virulence factors may cause its pathogenicity 6 , one example is the presence of lipopolysaccharide (LPS).The LPS is the primary element of the outer surface of P. aeruginosa that creates biofilm. A 2024, 21 (2): 0313-0327 https://doi.org /10.21123/bsj.2023.7709 P-ISSN: 2078-8665 -E-ISSN: 2411 Baghdad Science Journal biofilm is a collection of microorganisms that are enclosed in extracellular polymeric substances, which are made up of proteins, polysaccharides, and extracellular DNA 7 .…”