Isolation, screening and growth optimization of antagonistic Bacillus subtilis MS21 from Thengapattanam estuary against plant fungal pathogens

Anjhana, V.R.; Sasikala, S.

doi:10.22192/ijarbs.2017.04.12.003

Cited by 5 publications

(3 citation statements)

References 31 publications

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“…The highly efficient phosphate solubilizing bacterial isolate P III showing highest solubilization of phosphate in TCP medium (P III-34.17%) was identified by morphological examination, cultural studies and biochemical characterization as Bacillus subtilis according to the method given by Buchanon and Gibbons (1974) [3] and used for formulation of microbial consortium. The results of the present findings are in agreement with the findings of Anjhana and Sasikala, (2017) [2] and Vijayalakshmi and Murali (2015) [21] . The highly efficient potash mobilizing bacterial isolate K II was tested for different biochemical characters viz., sugar utilization, gram staining, gelatin liquefaction, catalase test, oxidase test, casein hydrolysis, methyl red test, Voges-Proskaeur test, urea hydrolysis, nitrate reduction, starch hydrolysis test and H 2 S production test (Table 4).…”

Section: Biochemical Characterization and Identification Of Nitrogen Fixing Phosphate Solubilizing And Potash Mobilizing Bacterial Isolatsupporting

confidence: 93%

Formulation of a compatible microbial consortia of nitrogen fixing, phosphate solubilising and potash mobilizing bacteria for optimizing nutrient supplementation to tuberose

Kore¹,

Tirmali²,

Jadhav³

et al. 2020

Int. J. Chem. Stud.

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The present investigation was conducted with the aim to isolate and characterize Azotobacter, phosphate solubilising bacteria (PSB) and potash mobilizing bacteria (KMB) from rhizosphere soil of tuberose and formulate consortium of Azotobacter, PSB and KMB. Three isolates each of Azotobacter, PSB and KMB were obtained from soil samples collected from three different locations of Pune region. Based on morphological and biochemical characterization, the highly efficient nitrogen fixing isolate (A I), PSB isolate (P III) and KMB isolate (K II) were identified as Azotobacter chroococcum, Bacillus subtilis and Frateuria aurantia respectively and used for the formulation of microbial consortium. Among the different culture media formulated, M III media having Glucose (10 g/L), Ammonium sulphate (0.4 g/L) and Yeast extract (1 g/L) recorded maximum growth and c.f.u. count of Azotobacter, PSB and KMB. These three beneficial organisms were found compatible with each other when grown on M III culture medium.

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Section: Biochemical Characterization and Identification Of Nitrogen Fixing Phosphate Solubilizing And Potash Mobilizing Bacterial Isolatsupporting

confidence: 93%

Formulation of a compatible microbial consortia of nitrogen fixing, phosphate solubilising and potash mobilizing bacteria for optimizing nutrient supplementation to tuberose

Kore¹,

Tirmali²,

Jadhav³

et al. 2020

Int. J. Chem. Stud.

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“…For example, the nitrogen-fixing B. subtilis AS-4 strain had the optimal growth at pH 7.0 (Satapute et al, 2012). B. subtilis MS21 antagonizing Gloeosporium gloeosporioide reached the highest cell density at pH 8.0 (Anjhana and Sasikala, 2017). Aspergillus flavuscontrolling B. subtilis UTB96 strain grew optimally in the pH 7.0 medium (Ghasemi and Ahmadzadeh, 2013).…”

Section: Discussion Discussion Discussionmentioning

confidence: 99%

Application of fruit juice for proliferation of Bacillus to control fungal phytopathogens

Tran

Nguyen³

et al. 2023

Not Bot Horti Agrobo

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Plant-based media have recently been of interest as potential natural media for microbial culture. This study was conducted to apply inexpensive and available fruits to culture Bacillus antagonizing three fungal phytopathogens including Phytophthora capsici GTC 2.6.1, Rhizoctonia solani GTC 2.7.1, and Sclerotium rolfsii GTC 2.9.1. The results showed that the juice media of dragon fruit, Cavendish banana, watermelon, pineapple, and MT1 seedless guava could all be used to culture two screened antagonistic Bacillus strains including B08 and B18. Surveys on the media from watermelon juice indicated that juice concentration (based on mass of fruit pulp), initial pH, concentration of traditional medium added to the juice medium had different effects on the growth of two strains of Bacillus sp. B08 and B18. These preliminary results demonstrated the potential application of the juice in biomass production of antagonistic Bacillus strains. This approach is certainly safe for the environment and has the potential to improve fruit consumption and reduce the cost of microbial fertilizer production, so it needs more research attention.

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“…amyloliquefaciens. Anjhana and Sasikala (2017) found 35°C to be ideal for the growth of B. subtilis.…”

Section: Effect Of Different Temperaturesmentioning

confidence: 99%

Antagonistic Potential of Salt Tolerant Bacteria and Optimization of Their Culture Conditions for Enhancement of the Activity

Verma¹,

Chandra²,

Prajapat³

et al. 2021

PLANT ARCHIVES

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The antagonistic potential of bacteria is being applied to biocontrol the infectious diseases caused by pathogenic fungi in plants that are one of the major threats to the growth and productivity of crop plants. In the present study, bacterial strains were isolated from soil samples collected from the rhizosphere of Sorghum (Sorghum bicolor) and Wheat (Triticum aestivum). Microscopic analysis revealed that all three bacterial isolates were Gram-positive, rod-shaped and spore-forming. The isolates Bacillus subtilis BP171 and Bacillus amyloliquefaciens BP124 demonstrated salt tolerance up to 12% while Bacillus subtilis BP67 tolerated up to 10% of NaCl. All the three strains were screened against seven test pathogenic fungi like Bipolaris sorokiniana, Fusarium oxysporum, Aspergillus sp., Penicillium sp., Rhizoctonia solani, Aspergillus niger, and Fusarium sp. for their antagonistic activity. BP124 was found to be the most potent in comparison to BP67 and BP171. Bacillus amyloliquefaciens BP124 demonstrated significantly highest (p<.0001) inhibition percentage against Fusarium sp., (61%) and Fusarium oxysporum (60%). The optimization of various parameters like pH, temperature, inoculum size, agitation, carbon sources, and nitrogen sources was carried out to enhance the antagonistic potential of bacterial isolates. The results revealed that the bacterial isolates were able to demonstrate significantly highest (p<.0001) antagonistic potential when inoculum size required for the growth was 1ml, agitation rate at 150 rpm, while the medium of pH at 7.0 and 30o C incubation temperature. Starch as carbon source and peptone as nitrogen source supported significantly highest (p<.0001) antagonistic activity against all the fungal pathogens for all the bacterial isolates. Therefore, the study showed that appropriate and optimum fermentation conditions can be of great importance in enhancing the antagonistic potential of bacterial isolates.

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Isolation, screening and growth optimization of antagonistic Bacillus subtilis MS21 from Thengapattanam estuary against plant fungal pathogens

Cited by 5 publications

References 31 publications

Formulation of a compatible microbial consortia of nitrogen fixing, phosphate solubilising and potash mobilizing bacteria for optimizing nutrient supplementation to tuberose

Formulation of a compatible microbial consortia of nitrogen fixing, phosphate solubilising and potash mobilizing bacteria for optimizing nutrient supplementation to tuberose

Application of fruit juice for proliferation of Bacillus to control fungal phytopathogens

Antagonistic Potential of Salt Tolerant Bacteria and Optimization of Their Culture Conditions for Enhancement of the Activity

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