Isolation, semi-synthesis and bio-evaluation of spatane derivatives from the brown algae Stoechospermum marginatum

Chinnababu, B.; Reddy, S. Purushotham; Rao, P. Shanthan; Reddy, V. L. Niranjan; Kumar, B. S. P. Anil; Rao, J. Venkateswara; Prakasham, R. S.; Babu, K. Suresh

doi:10.1016/j.bmcl.2015.04.061

Cited by 20 publications

(22 citation statements)

References 29 publications

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“…Recent report stated that S. marginatum having good antioxidant, antifungal, and antibacterial as well as cytotoxic activities. Also, a differential anti-microbial & anticancer property of S. marginatum was due to their phytonutrients and its analog against bacterial strains and cell lines (B16F10, U937, THP-1, COLO205 and HL60) [1]. Further studies are needed to identify bioactive molecules in the ethanol extract and evaluated its mechanisms in an animal model.…”

Section: Resultsmentioning

confidence: 99%

“…Among them, ethanol extract was found highest antibacterial activity and hexane lowest [2]. Antibacterial activity of S. marginatum was mainly due to the various phytonutrients like triterpenoids, phenolics, spatane derivatives and etc., which may affect growth and metabolism of bacteria [1]. Antibacterial activity of ethanol extract of S. marginatum was also recorded using both gram positive and negative bacteria's [16].…”

Section: Discussionmentioning

confidence: 99%

“…Cancer is one of the most serious threats against human health and also has done extensive research to the development of an overabundance of chemotherapeutic agents. However, none of these agents are capable of completely eliminating cancer [1]. On other hand, many drug resistance mechanisms in human pathogenic microorganisms have developed because of the arbitrary use of known antibacterial drugs against infectious diseases.…”

Section: Introductionmentioning

confidence: 99%

“…Also, more secondary metabolites found in S. marginatum and reported to be enhanced peroxidase, phenyl alanine lyase, catalase, and poly phenol oxidase activities [8]. Biological activity of spatane derivatives obtained from S. marginatum was well regarded [1]. Hence, in the present study aimed to screen antibacterial and antioxidant activities of ethanol extract and its fraction of S. marginatum and also anticancer properties in HepG2 cell line studied by analyzing cytotoxicity using MTT assay and apoptosis by flowcytometer.…”

Section: Introductionmentioning

confidence: 99%

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Determination of Various Bioactive Potential of Stoechospermum marginatum (C. Agardh) Kutzing In-Vitro

Arumugam

Murugan

Kamalakannan

et al. 2017

JAPLR

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The objective of present study was to screen the antibacterial, total antioxidant and cytotoxic properties of ethanol extract and its fractions of S. marginatum. The highest antibacterial activity was found in ethanol extract followed by dichloromethane, ethyl acetate, hexane and aqueous fractions. TAAs and RAAs were estimated along with known antioxidant such as quercetin, β-carotene and L-ascorbic acid. TAA was found to be highest in EE (22-75%) followed by DMF (18-65%), EAF (13-50%), HF (8-38%) and AF (5-31%). R square value between concentration and TAA of extract/fractions made by linear regression clearly shows TAA dependents on the concentration. Moreover, ethanol extract exhibited cytotoxicity as equal as quercetin at their maximum concentration (640µg/mL and 160µg/mL). Cytotoxicity revealed timedependent growth inhibition of cancer cells. However, treatment with ethanol extract shows concentration dependent apoptotic cells. The present result and also other recent report clearly exhibited that the S. marginatum having potential antibacterial and antioxidant as well as cytotoxic properties.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Determination of Various Bioactive Potential of Stoechospermum marginatum (C. Agardh) Kutzing In-Vitro

Arumugam

Murugan

Kamalakannan

et al. 2017

JAPLR

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“…In our recent paper, we described the ten structures of a new cytotoxic diterpinoids from extracts of the brown algae Stoechospermum marginatum [23]. In the present study, we have evaluated the apoptotic effect of a spatane diterpinoid, 5(R), 19-diacetoxy-15,18(R and S), dihydro spata-13,16(E)-diene (DDSD) on mouse melanoma cells (B16F10) through ROS generation, cell cycle arrest, release of cytochrome c from mitochondria, depolarization of mitochondrial membrane potential and activation of caspase cascade, and studied the tumor growth inhibition potentiality in C57BL/6 mice.…”

Section: Introductionmentioning

confidence: 99%

Spatane diterpinoid from the brown algae, Stoechospermum marginatum induces apoptosis via ROS induced mitochondrial mediated caspase dependent pathway in murine B16F10 melanoma cells

Velatooru

Baggu

Janapala

2016

Molecular Carcinogenesis

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Spatane diterpinoids isolated from the brown marine algae Stoechospermum marginatum were known to have cytotoxic effects in human cancerous cell lines and murine melanoma cells; the underling apoptotic mechanism of diterpinoids still remains unclear so far. Thus, in the present study, the apoptotic mechanism of a spatane diterpinoid, 5(R), 19-diacetoxy-15,18(R and S), dihydro spata-13, 16(E)-diene (DDSD) was investigated mainly in B16F10 melanoma cells because they were most susceptible to DDSD than THP1, U937, COLO205, and HL60 cells. The treatment of B6F10 cells with DDSD resulted in morphological alterations, nuclear condensation, and DNA fragmentation, which leads to cell growth inhibition in a concentration-dependent manner. Data indicate that DDSD induced the generation of ROS, consequentially caused alteration in Bax/Bcl-2 ratio that disrupted the inner mitochondrial transmembrane potential (ΔΨm) resulting in cytochrome c redistribution to the cytoplasm and activation of caspase-mediated apoptotic pathway. Flow cytometric analysis clearly indicated that the DDSD inducing phosphatidylserine externalization and mediated "S-phase" arrest in cell cycle. In addition, results also found that DDSD induced apoptosis through deregulating PI3K/AKT signaling pathway. The anti-tumor activity of DDSD was evaluated in C57BL/6 mice bearing B16F10 melanoma. It effectively inhibited tumor growth (volume and weight) in a dose dependent manner, yet without apparent toxic effects. Morphology and apoptotic status of tumor tissues in the treated mice were assessed by microscopy and TUNEL assay, respectively. Our study shows a therapeutic potential of DDSD for the treatment of malignant melanoma and a new source of anticancer drugs. © 2016 Wiley Periodicals, Inc.

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Antioxidant, anti-inflammatory and anticancer potential of natural bioactive compounds from seaweeds

Sakthivel

Devi

2019

Studies in Natural Products Chemistry

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Isolation, semi-synthesis and bio-evaluation of spatane derivatives from the brown algae Stoechospermum marginatum

Cited by 20 publications

References 29 publications

Determination of Various Bioactive Potential of Stoechospermum marginatum (C. Agardh) Kutzing In-Vitro

Determination of Various Bioactive Potential of Stoechospermum marginatum (C. Agardh) Kutzing In-Vitro

Spatane diterpinoid from the brown algae, Stoechospermum marginatum induces apoptosis via ROS induced mitochondrial mediated caspase dependent pathway in murine B16F10 melanoma cells

Antioxidant, anti-inflammatory and anticancer potential of natural bioactive compounds from seaweeds

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