Isolation, structural analysis, and expression characteristics of the maize TIFY gene family

Abstract

TIFY, previously known as ZIM, comprises a plant-specific family annotated as transcription factors that might play important roles in stress response. Despite TIFY proteins have been reported in Arabidopsis and rice, a comprehensive and systematic survey of ZmTIFY genes has not yet been conducted. To investigate the functions of ZmTIFY genes in this family, we isolated and characterized 30 ZmTIFY (1 TIFY, 3 ZML, and 26 JAZ) genes in an analysis of the maize (Zea mays L.) genome in this study. The 30 ZmTIFY genes were distributed over eight chromosomes. Multiple alignment and motif display results indicated that all ZmTIFY proteins share two conserved TIFY and Jas domains. Phylogenetic analysis revealed that the ZmTIFY family could be divided into two groups. Putative cis-elements, involved in abiotic stress response, phytohormones, pollen grain, and seed development, were detected in the promoters of maize TIFY genes. Microarray data showed that the ZmTIFY genes had tissue-specific expression patterns in various maize developmental stages and in response to biotic and abiotic stresses. The results indicated that ZmTIFY4, 5, 8, 26, and 28 were induced, while ZmTIFY16, 13, 24, 27, 18, and 30 were suppressed, by drought stress in the maize inbred lines Han21 and Ye478. ZmTIFY1, 19, and 28 were upregulated after infection by three pathogens, whereas ZmTIFY4, 13, 21, 23, 24, and 26 were suppressed. These results indicate that the ZmTIFY family may have vital roles in response to abiotic and biotic stresses. The data presented in this work provide vital clues for further investigating the functions of the genes in the ZmTIFY family.

RNA‐Seq identification of candidate defense genes by analyzing Mythimna separata feeding‐damage induced systemic resistance in balsas teosinte

“…22 Zhang et al isolated and characterized 30 ZmTIFYs and concluded that they had tissue-specific expression patterns in various developmental stages and in response to biotic and abiotic stresses. 23 Here, we identified two TIFY genes whose expressions were up-regulated by 293.7-and 144.8-fold in teosinte plants that were involved in the armyworm attacking response.…”

A Key Role for Apoplastic H2O2 in Norway Spruce Phenolic Metabolism

“…10C; Table II). The Tify transcription factor family has been connected to a variety of abiotic and biotic stresses and also to development (Zhang et al, 2015). In maize (Zea mays), ZML2, a Tify family member, and MYB11 together repress COMT expression (Vélez-Bermúdez et al, 2015).…”

Comprehensive Analysis of the TIFY Gene Family and Its Expression Profiles under Phytohormone Treatment and Abiotic Stresses in Roots of Populus trichocarpa

“…The TIFY gene family is specific to land plants and was first found in Arabidopsis and annotated as a transcription factor [1,2]. This gene family contains a core motif, TIF[F/Y]XG, previously known as ZIM (a zinc-finger protein expressed in inflorescence meristem) [1], and includes 20 members in Rice, 18 members in Arabidopsis, and 27 members in Maize [3][4][5]. Depending on the conservative domain, the proteins are divided into two groups: Group I containing the C2C2-GATA (CX2CX20CX2C) conservative domain, which is absent in Group II.…”

“…In Arabidopsis, AtPPD2 interacts with AtLHP1 to directly or indirectly regulate several target genes to affect lateral organ development, while Arabidopsis ppd2 shows greater leaf breadth than wild-type (WT) [17]. In Maize, ZmTIFY4, 5, 8, 26, and 28 respond to drought; additionally, ZmTIFY1 19 and 28 show upregulation when treated by three kinds of pathogens [5]. In summary, different TIFY subfamily members have different functions during plant growth and development, as well as different responses to phytohormone treatment and abiotic stress.Populus trichocarpa serves as a model forest species and is widely used in forest tree genomics studies [18].…”