Isolation, structure determination and antibacterial activities of succinamide conjugate diacid from Acinetobacter sp. BJ-L

Abstract

Strain BJ-L was isolated from the a water sample taken from Xiao Yue River in Beijing and identified as Acinetobacter sp. BJ-L based on the study of its morphology, physiology, biochemistry and 16S rRNA gene sequence. A new antimicrobial substance was produced after the strain was incubated in potato extract medium at 15°C for 72 h. The antimicrobial substance was sequentially purified by reduced pressure condensation, EtOAc extract, and silica gel column chromatography. The structure of the antimicrobial substance was elucidated as succinamide conjugate diacid (SCD) by spectroscopic data interpretation. Structure analysis indicated that SCD is a novel compound and that it could inhibit the growth of some tested bacterial strains with the MIC of 2mg/ml, such as Staphylococcus aureus, Bacillus subtilis, Enterobacter aerogenes and Escherichia coli. Moreover, no obvious toxicity has been found on cultured HUVEC cells with different concentrations of SCD at 5, 10, 15, and 20mg/ml.

Diversity of the microbial community and antioxidant activity during fermentation of red raspberry Enzymes

“…Furthermore, the remaining major genera, Akkermansia , Streptococcus , Prevotella , Acinetobacter , etc., were present in our samples. Among them, Acinetobacter which can produce antibacterial substances has been reported (Wu et al., 2010). Prevotella has been found to have antitumor effects (Xin et al., 2019).…”

Isolation and Description of Antimicrobial and Anti-Tumor Produced by Actinomyces Sp.

“…griseus (HU7) strains and the three antibiotic-producing isolates yielded multiple ITS bands ( Figure 1, lanes 2, 3, 4 From the view of Zoology Department: NHP showed both high antimicrobial activity and high cancer cell cytotoxicity. Additionally, NHP is a small molecule, which can permeate the tissues and organs easily (Wu et al 2011) and can affect more than one pathway or interact with multiple targets (Maheshwari et al 2012). Therefore, NHP might be a special attractive candidate for chemoprevention and have potential applications both in clinical treatment and certain industrial processes as a broad antibacterial substance.…”

Novel Antimicrobial Indolepyrazines A and B from the Marine-Associated Acinetobacter sp. ZZ1275

“…The species Acinetobacter baumannii , in particular, is known to cause nosocomial infections, such as endocarditis, meningitis, pneumonia, septicaemia, and tract infection [1]. Some Acinetobacter bacteria were reported to produce secondary metabolites, mainly including siderophores acinetobactin [2], acinetoferrin [3], baumannoferrins A and B [4], polysaccharides [5,6,7], antibacterial succinamide conjugate diacid [8], antifungal volatile compounds [1], and antimicrobial diketopiperazines cyclo-(proline-leucine) and cyclo-(proline-tyrosine) [9]. However, it is our understanding that no natural product has yet been reported from marine-derived Acinetobacter species.…”